FastQCFastQC Report
Thu 26 May 2016
SRR1926943_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926943_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences640995
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15150.2363512975920249No Hit
CTGTAGGACGTGGAATATGGCAAGA12520.195321336359878No Hit
GTCCTAAAGTGTGTATTTCTCATTT12340.19251320213106185No Hit
GTCCTACAGTGTGCATTTCTCATTT10600.16536790458583922No Hit
CTTTAGGACGTGAAATATGGCGAGG9360.14602297989843915No Hit
GTATCAACGCAGAGTACTTTTTTTT8770.1368185399262085No Hit
CTGTAGGACCTGGAATATGGCGAGA7060.11014126475245517No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC508.391252E-515.2647471
TGCCGGT456.8381085E-414.75386210
GGTGGCC400.005238431514.2647898
GGTTAGA551.8879754E-413.8770431
GTATAAC551.9031216E-413.8639823
CATCTAA706.93517E-613.6292381
GTCTTAC500.001450632313.3566531
ACTATAC500.00146096613.3440833
TAAGGTG500.001477456913.3242295
CCGGTTT500.001519832213.27433412
TAGGACC2800.013.2645584
TCTAGAC655.276271E-513.1974443
TAAAGGT655.3191514E-513.18606954
GTGTAGG1102.564775E-913.00972751
AATCCCG701.1071713E-412.19261619
TCCAACG701.1071713E-412.19261618
GTATTAC550.002968982812.1424121
CGTGTGC550.003041328912.1034578
TACACTG2300.011.5862865
CTAGACT752.0236503E-411.4279274
Produced by FastQC (version 0.11.5)