FastQCFastQC Report
Thu 26 May 2016
SRR1926943_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926943_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences640995
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15250.23791137216358943No Hit
CTGTAGGACGTGGAATATGGCAAGA15200.23713133487780713No Hit
GTATCAACGCAGAGTACTTTTTTTT14670.2288629396485152No Hit
GTCCTAAAGTGTGTATTTCTCATTT11730.18299674724451828No Hit
CTTTAGGACGTGAAATATGGCGAGG11710.18268473233020538No Hit
GTCCTACAGTGTGCATTTCTCATTT11130.17363629981513115No Hit
GGTATCAACGCAGAGTACTTTTTTT9220.1438388754982488No Hit
CTGTAGGACCTGGAATATGGCGAGA8280.12917417452554233No Hit
TATCAACGCAGAGTACTTTTTTTTT7960.12418193589653587No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATA456.6236994E-414.8159724
TAGGACC2750.013.8538964
TCCAACG707.352568E-613.55237218
GCAATAC550.003002828512.1240583
AGGACGT8400.011.9056925
GTAGGAC8750.011.8667323
CTGTAGG8700.011.7297241
TAGGACT1302.4974725E-911.72252754
CGGTTTC658.1071357E-411.6758913
GCCGGTT658.1071357E-411.6758911
GTGTAAG907.1239156E-611.6566421
TGTAGGA8850.011.6377682
GGACGTG8450.011.6098116
AGGACCT4700.011.5510545
GACGTGA4550.011.46828657
GCAGGAC752.0181859E-411.4312553
CCGGTTT752.0973849E-411.38399212
GGCGAGG3350.011.32735619
TAGGACG9050.011.2610744
TTAGGAC4800.011.113723
Produced by FastQC (version 0.11.5)