##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926943_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 640995 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.842146974625386 31.0 30.0 33.0 26.0 34.0 2 30.13922261484099 31.0 30.0 34.0 26.0 34.0 3 30.183508451703993 31.0 30.0 34.0 26.0 34.0 4 33.764697072520065 35.0 35.0 37.0 28.0 37.0 5 33.79874257989532 37.0 35.0 37.0 30.0 37.0 6 33.751625207684924 37.0 35.0 37.0 29.0 37.0 7 33.749611151413035 37.0 35.0 37.0 29.0 37.0 8 33.790252654076866 37.0 35.0 37.0 29.0 37.0 9 35.31134096209799 39.0 35.0 39.0 30.0 39.0 10 35.19932448771051 39.0 35.0 39.0 29.0 39.0 11 35.239828703812044 39.0 35.0 39.0 30.0 39.0 12 35.148232045491774 39.0 35.0 39.0 29.0 39.0 13 35.151586205820635 39.0 35.0 39.0 29.0 39.0 14 36.24945592399317 40.0 36.0 41.0 29.0 41.0 15 36.244161030897274 40.0 36.0 41.0 29.0 41.0 16 36.22886605979766 40.0 36.0 41.0 29.0 41.0 17 36.2050967636253 40.0 36.0 41.0 29.0 41.0 18 36.17841168807869 40.0 36.0 41.0 29.0 41.0 19 36.24150266382733 40.0 37.0 41.0 29.0 41.0 20 36.20746651689951 40.0 37.0 41.0 29.0 41.0 21 36.15285766659646 40.0 37.0 41.0 29.0 41.0 22 36.06210500861941 40.0 37.0 41.0 28.0 41.0 23 35.98931036903564 40.0 36.0 41.0 28.0 41.0 24 35.92619443209385 40.0 36.0 41.0 27.0 41.0 25 35.80427304425152 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.001264237422592629 1101 2 -0.07941228623452545 1101 3 0.1549873687685519 1101 4 0.03525568484581498 1101 5 0.13348833428055684 1101 6 0.10041593074463862 1101 7 0.2293025149835941 1101 8 0.08275490979519873 1101 9 0.24209632813219883 1101 10 0.08188908706785725 1101 11 0.20223408232105555 1101 12 0.30241737751379105 1101 13 0.3655612746812551 1101 14 0.08656321218723662 1101 15 0.3359718305609789 1101 16 0.20643185348939852 1101 17 0.17417079516201994 1101 18 -0.05037687912034983 1101 19 -6.657034823192021E-4 1101 20 0.14341001356939387 1101 21 0.15038015924164227 1101 22 0.014507364005304169 1101 23 -0.01606151526054589 1101 24 0.08524476475094644 1101 25 0.3158523151827666 1102 1 -0.4333388268361489 1102 2 -0.4106816190878071 1102 3 0.07670646419408911 1102 4 -0.0066602461936611235 1102 5 -0.03916982465004537 1102 6 -0.13050587726270635 1102 7 -0.03570438941084575 1102 8 0.02505107648418914 1102 9 -0.023568085367486447 1102 10 -0.06542917587870534 1102 11 -0.008719865697308649 1102 12 -0.1801756452059493 1102 13 -0.04030405779442958 1102 14 -0.03299916555887705 1102 15 0.012459787935945599 1102 16 0.14930759414760786 1102 17 0.3089733338716343 1102 18 -0.1588263650777506 1102 19 0.04178150369561706 1102 20 -0.05462755775223371 1102 21 0.084935500871957 1102 22 0.030334782683837602 1102 23 0.06521714785549904 1102 24 0.027989881730491106 1102 25 -0.024984378874627566 1103 1 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1107 9 -0.34734486222196637 1107 10 -0.1863710833998269 1107 11 -0.17652533320004693 1107 12 -0.2770779111294033 1107 13 -0.2981768883211444 1107 14 -0.2995677212293515 1107 15 -0.37446326800829866 1107 16 -0.2732826200672278 1107 17 -0.4065946364894728 1107 18 -0.4750287352659939 1107 19 -0.5831275006281231 1107 20 -0.5187180939921134 1107 21 -0.722014156229065 1107 22 -0.629492435670123 1107 23 -0.30712352317336666 1107 24 -0.4326485879897177 1107 25 -0.4061749694496726 1108 1 0.6575465673859497 1108 2 0.4899435530237142 1108 3 0.7405713479780829 1108 4 0.7890425889429409 1108 5 0.8812416033448045 1108 6 0.7841257567824798 1108 7 0.6753868449361988 1108 8 0.5561310013962668 1108 9 0.47668311862486945 1108 10 0.45174098487082404 1108 11 0.5344630465452624 1108 12 0.38804673444211346 1108 13 0.3386261285169496 1108 14 0.27376925799417506 1108 15 0.39076248979780104 1108 16 0.696351059795731 1108 17 0.4029919460904452 1108 18 0.5092595866280192 1108 19 0.48804592164860594 1108 20 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1110 8 0.5046610240300069 1110 9 0.4694158034079834 1110 10 0.4385538421323645 1110 11 0.4020455320420382 1110 12 0.3127728297064536 1110 13 0.3858976179062452 1110 14 0.5349954515339377 1110 15 0.5450467914648343 1110 16 0.45829354942615197 1110 17 0.4772891755341675 1110 18 0.43668065728297734 1110 19 0.23764724765754863 1110 20 0.6025870794223209 1110 21 0.6034298700677283 1110 22 0.7609686075060367 1110 23 0.7160489570039346 1110 24 0.737279562964055 1110 25 0.5108336188672169 1111 1 0.3380762104016739 1111 2 0.2559017120524878 1111 3 0.31341006165718 1111 4 0.3247168953100612 1111 5 0.3631039193958543 1111 6 0.3612570638673205 1111 7 0.29097526005361374 1111 8 0.25407026119957266 1111 9 0.22972805400460317 1111 10 0.4468606212212194 1111 11 0.3424472702799548 1111 12 0.6104109491781173 1111 13 0.3957205744327368 1111 14 0.26952195341793583 1111 15 0.3543108892807183 1111 16 0.22582276559948866 1111 17 0.3516718259372169 1111 18 0.4214652215429453 1111 19 0.33699979891390797 1111 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-0.6609527018658525 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15822.0 3 8.0 4 236.0 5 1570.0 6 2278.0 7 1673.0 8 1243.0 9 1105.0 10 1027.0 11 875.0 12 855.0 13 748.0 14 776.0 15 784.0 16 851.0 17 887.0 18 955.0 19 1107.0 20 1497.0 21 1902.0 22 2491.0 23 3041.0 24 3582.0 25 4252.0 26 5117.0 27 5528.0 28 6544.0 29 7960.0 30 10243.0 31 13045.0 32 17150.0 33 22276.0 34 30532.0 35 42607.0 36 62923.0 37 108959.0 38 258546.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.261166989442124 19.90675954270007 10.33532204660461 28.4967514212532 2 13.964449358607958 21.1881135546581 40.50421191705475 24.343225169679194 3 18.439300225310973 25.01981613126705 29.38919505938598 27.151688584036 4 11.71756409956396 17.857549590870445 38.56941161787533 31.85547469169026 5 12.453586116086194 38.97174915596937 35.931522749823706 12.643141978120727 6 31.12824325910852 38.72844675771925 17.854498493327593 12.288811489844637 7 27.60094997050847 32.0153168700906 22.207901233026767 18.175831926374165 8 24.372042369879875 39.07324859149326 20.02178095165284 16.532928086974024 9 26.001163935474914 15.795665354508845 20.199585289478243 38.003585420538 10 14.064350249079116 30.3310185799136 34.7855797778648 20.819051393142484 11 33.829620927391616 23.12129168948404 24.832009535711332 18.217077847413016 12 23.117761220108832 25.393920672956916 32.16561205132045 19.322706055613796 13 28.85501042147698 22.784812101394106 25.752592888434034 22.60758458869488 14 19.90374339893447 23.39909047652478 27.894757369402257 28.802408755138497 15 22.073353188316034 32.64932969932026 23.214728673704837 22.062588438658867 16 21.02401612515718 29.27661446583857 28.798600905479994 20.900768503524258 17 20.694265468943545 29.83491265128847 28.489190226429578 20.981631653338408 18 20.476762507995446 28.466278725097116 30.688154261376933 20.36880450553051 19 22.712069067729182 27.6718658833 27.914613865340392 21.701451183630425 20 22.799009023626173 28.438931392983285 28.514596308176177 20.247463275214358 21 21.858539081808424 27.5613500761415 27.691009062086525 22.889101779963553 22 22.142640960757824 29.414159882931823 27.60712268757586 20.836076468734497 23 21.025157801789096 29.04800980976421 28.417270676480594 21.509561711966104 24 22.301300792207105 27.59604614083751 29.247543658755 20.85510940820039 25 21.62066022737733 28.4366755543629 28.69410151319659 21.24856270506318 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 232.0 1 232.0 2 227.5 3 223.0 4 223.0 5 223.0 6 422.0 7 621.0 8 621.0 9 621.0 10 968.0 11 1315.0 12 1315.0 13 1315.0 14 2281.0 15 3247.0 16 3247.0 17 3247.0 18 5520.0 19 7793.0 20 7793.0 21 7793.0 22 12506.0 23 17219.0 24 17219.0 25 17219.0 26 24799.5 27 32380.0 28 32380.0 29 32380.0 30 42824.5 31 53269.0 32 53269.0 33 53269.0 34 60639.5 35 68010.0 36 68010.0 37 68010.0 38 72737.0 39 77464.0 40 77464.0 41 77464.0 42 80351.5 43 83239.0 44 83239.0 45 83239.0 46 84546.5 47 85854.0 48 85854.0 49 85854.0 50 80953.0 51 76052.0 52 76052.0 53 76052.0 54 65400.5 55 54749.0 56 54749.0 57 54749.0 58 45928.0 59 37107.0 60 37107.0 61 37107.0 62 29652.5 63 22198.0 64 22198.0 65 22198.0 66 16990.0 67 11782.0 68 11782.0 69 11782.0 70 8477.0 71 5172.0 72 5172.0 73 5172.0 74 3572.0 75 1972.0 76 1972.0 77 1972.0 78 1371.5 79 771.0 80 771.0 81 771.0 82 511.5 83 252.0 84 252.0 85 252.0 86 157.0 87 62.0 88 62.0 89 62.0 90 35.0 91 8.0 92 8.0 93 8.0 94 5.5 95 3.0 96 3.0 97 3.0 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11154533186686323 2 0.10436898883766643 3 0.016068768087114563 4 0.0 5 0.003588171514598398 6 0.33853618202950103 7 0.020748991801808128 8 0.010608507086638741 9 0.010296492172325837 10 0.0053042535433193705 11 0.005616268457632275 12 4.6802237146935626E-4 13 0.0017160820287209727 14 0.0 15 0.002184104400190329 16 0.0014040671144080687 17 1.560074571564521E-4 18 7.800372857822604E-4 19 1.560074571564521E-4 20 0.0017160820287209727 21 0.012948618943985522 22 1.560074571564521E-4 23 1.560074571564521E-4 24 1.560074571564521E-4 25 0.0053042535433193705 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 640995.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.7663516239458 #Duplication Level Percentage of deduplicated Percentage of total 1 92.2952265742824 77.31234402433086 2 6.011681554381483 10.071532618710167 3 0.8444970218590251 2.1222130343525447 4 0.30485739579445326 1.0214716724511435 5 0.14661104228735755 0.614053606009799 6 0.07942817956631315 0.39920452910410176 7 0.06117523841219514 0.35870985720602583 8 0.03693561885628121 0.24751696292506356 9 0.03086812570563958 0.2327139244647689 >10 0.1571834926290226 2.5103429787774254 >50 0.01642109319340569 0.9792840378844982 >100 0.012688852916719508 2.1479936602703007 >500 0.0013062054469631585 0.7369953505136697 >1k 0.0011196046688255645 1.245623742999668 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1525 0.23791137216358943 No Hit CTGTAGGACGTGGAATATGGCAAGA 1520 0.23713133487780713 No Hit GTATCAACGCAGAGTACTTTTTTTT 1467 0.2288629396485152 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1173 0.18299674724451828 No Hit CTTTAGGACGTGAAATATGGCGAGG 1171 0.18268473233020538 No Hit GTCCTACAGTGTGCATTTCTCATTT 1113 0.17363629981513115 No Hit GGTATCAACGCAGAGTACTTTTTTT 922 0.1438388754982488 No Hit CTGTAGGACCTGGAATATGGCGAGA 828 0.12917417452554233 No Hit TATCAACGCAGAGTACTTTTTTTTT 796 0.12418193589653587 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGATA 45 6.6236994E-4 14.815972 4 TAGGACC 275 0.0 13.853896 4 TCCAACG 70 7.352568E-6 13.552372 18 GCAATAC 55 0.0030028285 12.124058 3 AGGACGT 840 0.0 11.905692 5 GTAGGAC 875 0.0 11.866732 3 CTGTAGG 870 0.0 11.729724 1 TAGGACT 130 2.4974725E-9 11.7225275 4 CGGTTTC 65 8.1071357E-4 11.67589 13 GCCGGTT 65 8.1071357E-4 11.67589 11 GTGTAAG 90 7.1239156E-6 11.656642 1 TGTAGGA 885 0.0 11.637768 2 GGACGTG 845 0.0 11.609811 6 AGGACCT 470 0.0 11.551054 5 GACGTGA 455 0.0 11.4682865 7 GCAGGAC 75 2.0181859E-4 11.431255 3 CCGGTTT 75 2.0973849E-4 11.383992 12 GGCGAGG 335 0.0 11.327356 19 TAGGACG 905 0.0 11.261074 4 TTAGGAC 480 0.0 11.11372 3 >>END_MODULE