FastQCFastQC Report
Thu 26 May 2016
SRR1926942_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926942_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450116
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT9350.20772423108709756No Hit
GTCCTACAGTGGACATTTCTAAATT8480.18839588017311093No Hit
GTCCTACAGTGTGCATTTCTCATTT7040.15640412693616756No Hit
CTGTAGGACGTGGAATATGGCAAGA6460.1435185596601765No Hit
GGTATCAACGCAGAGTACTTTTTTT5610.12463453865225853No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG5280.11730309520212567No Hit
GTCCTAAAGTGTGTATTTCTCATTT5180.1150814456717824No Hit
CACACACACACACACACACACACAC4940.1097494867989585No Hit
CTGTAGGACCTGGAATATGGCGAGA4930.10952732184592417No Hit
TATCAACGCAGAGTACTTTTTTTTT4870.10819433212771819No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATACT508.530352E-515.2334714
CTTACTG400.00516811314.29254153
GTAGGAC4250.013.9001963
CAGGACC707.072471E-613.6013144
TGTAGGA4700.013.3802522
TAGGCTG500.001473279913.3278015
TAAGCTG500.001473279913.3278015
GACGTGG1500.013.32787
TAGGACC2300.013.2464964
GTCCTAC4850.012.9794711
CTGTAGG4650.012.717261
ACGTGGA1600.012.4850638
CCTACAG5050.012.2642273
GGACGTG2900.012.1460256
ATAAGAG550.00299496412.1270053
TCCTACA5450.011.7137662
AGGACCT2850.011.6910535
TAGGACG3100.011.6708044
GTCCTAA2000.011.4455351
GTAGAAC1751.8189894E-1211.4340333
Produced by FastQC (version 0.11.5)