##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926942_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 450116 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.368011801402304 31.0 27.0 31.0 16.0 33.0 2 27.95055941135174 31.0 28.0 31.0 16.0 34.0 3 27.87237734272943 31.0 28.0 31.0 16.0 34.0 4 31.60186485261577 35.0 32.0 37.0 19.0 37.0 5 31.409638848652346 35.0 32.0 37.0 17.0 37.0 6 31.07974166659261 35.0 32.0 37.0 11.0 37.0 7 31.171711292200232 35.0 32.0 37.0 13.0 37.0 8 31.01145926827751 35.0 32.0 37.0 11.0 37.0 9 32.95025282371655 37.0 33.0 39.0 17.0 39.0 10 33.045388299904914 37.0 33.0 39.0 17.0 39.0 11 33.20905055585671 38.0 33.0 39.0 17.0 39.0 12 33.05683423828524 38.0 33.0 39.0 16.0 39.0 13 33.176007962391914 38.0 34.0 39.0 17.0 39.0 14 34.18830923584143 39.0 34.0 41.0 17.0 41.0 15 33.761948031174185 39.0 33.0 41.0 10.0 41.0 16 33.83719974406598 39.0 33.0 41.0 11.0 41.0 17 34.06794692923602 39.0 34.0 41.0 11.0 41.0 18 34.11672102302518 39.0 34.0 41.0 11.0 41.0 19 33.51994374783389 39.0 33.0 41.0 10.0 41.0 20 33.78090758826614 39.0 34.0 41.0 10.0 41.0 21 33.783306969758904 39.0 34.0 41.0 2.0 41.0 22 33.79879631028446 39.0 34.0 41.0 2.0 41.0 23 33.78871890801482 39.0 34.0 41.0 2.0 41.0 24 32.518977330288195 39.0 32.0 41.0 2.0 41.0 25 33.09829466182051 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -1.2252309640556867 1101 2 -1.0530152949392928 1101 3 -2.0049378362431334 1101 4 -1.5562121128325046 1101 5 -1.080363601270598 1101 6 -2.999438123910231 1101 7 -1.2771079180935097 1101 8 -1.0029828917031445 1101 9 -0.975656864620909 1101 10 -9.524306489688072 1101 11 -4.668458947591645 1101 12 -2.4893340093467664 1101 13 -1.8687288979362897 1101 14 -1.2477672450827342 1101 15 -2.926982283141104 1101 16 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-3.3462437006029866 2216 12 -2.7167832972190133 2216 13 -2.675716774108455 2216 14 -2.6839190087269458 2216 15 -3.6982146761257617 2216 16 -2.8884296595394474 2216 17 -3.056427098125301 2216 18 -2.830096215445902 2216 19 -2.241946698470745 2216 20 -3.4978246313342716 2216 21 -5.509526973698264 2216 22 -4.48087845990937 2216 23 -4.561721042464601 2216 24 -4.329474924230464 2216 25 -3.958567130812078 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27875.0 3 3.0 4 213.0 5 1345.0 6 1661.0 7 1475.0 8 1177.0 9 1062.0 10 996.0 11 971.0 12 916.0 13 874.0 14 858.0 15 892.0 16 934.0 17 1076.0 18 1144.0 19 1484.0 20 1837.0 21 2378.0 22 3072.0 23 3620.0 24 4216.0 25 4814.0 26 5416.0 27 5731.0 28 6497.0 29 7816.0 30 9108.0 31 10982.0 32 14211.0 33 18862.0 34 25188.0 35 35153.0 36 51356.0 37 84321.0 38 110582.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.47658641377545 18.57303458331942 12.863837223539365 25.086541779365767 2 13.825674154553907 19.926989762549884 43.673113457286114 22.574222625610087 3 19.672977241885608 24.316033013726056 29.062979688994684 26.948010055393652 4 10.91718785086832 16.73607143095356 39.711234678518466 32.63550603965965 5 12.41311265577501 38.6133135410926 36.4233524737338 12.550221329398589 6 31.454774137348668 37.754217702796396 18.854553696823167 11.936454463031769 7 27.85779239883388 31.709638307166994 22.008032665579 18.424536628420125 8 23.764611493074515 38.907077032794376 21.154210450072988 16.174101024058125 9 25.78174303364295 16.081507488556067 20.571530154215367 37.565219323585616 10 13.479749927213975 30.95084109532414 35.36711938464137 20.20228959282052 11 34.24000959931738 23.421001173249902 24.12384008248302 18.215149144949695 12 22.247085148981274 25.97911662292816 32.69255126960178 19.081246958488787 13 28.610346352378603 22.801883417438276 25.973428615234884 22.614341614948238 14 19.10360535525804 23.70268318426754 28.93505916337981 28.258652297094606 15 22.666681480329306 32.29104402990878 23.416178741653425 21.626095748108483 16 20.40629485931674 30.088352021474694 29.311049780231013 20.19430333897755 17 20.312743045833724 29.916581114533198 28.698852045918162 21.07182379371492 18 19.70966896128771 29.35529888587186 30.903390380367878 20.031641772472547 19 22.468739097294907 28.14203460865816 28.477365706450847 20.91186058759608 20 21.93527456313441 28.767132419604284 29.660418111034076 19.637174906227223 21 22.00068438969327 27.919766418686503 28.249295611906604 21.830253579713624 22 20.94532736031856 29.697527242991484 28.996009101736796 20.36113629495316 23 20.91595929069819 29.00626638816053 28.819163592729215 21.25861072841207 24 21.645968184555358 28.17037861667922 29.811147182539948 20.37250601622548 25 21.088406653719495 28.714052716017015 29.39377913668745 20.80376149357604 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 117.0 1 117.0 2 185.5 3 254.0 4 254.0 5 254.0 6 418.5 7 583.0 8 583.0 9 583.0 10 897.0 11 1211.0 12 1211.0 13 1211.0 14 1987.0 15 2763.0 16 2763.0 17 2763.0 18 4504.0 19 6245.0 20 6245.0 21 6245.0 22 9647.5 23 13050.0 24 13050.0 25 13050.0 26 18388.5 27 23727.0 28 23727.0 29 23727.0 30 30903.0 31 38079.0 32 38079.0 33 38079.0 34 43149.5 35 48220.0 36 48220.0 37 48220.0 38 52208.5 39 56197.0 40 56197.0 41 56197.0 42 58336.0 43 60475.0 44 60475.0 45 60475.0 46 61095.5 47 61716.0 48 61716.0 49 61716.0 50 57619.0 51 53522.0 52 53522.0 53 53522.0 54 45545.5 55 37569.0 56 37569.0 57 37569.0 58 30613.5 59 23658.0 60 23658.0 61 23658.0 62 18276.5 63 12895.0 64 12895.0 65 12895.0 66 9488.5 67 6082.0 68 6082.0 69 6082.0 70 4288.5 71 2495.0 72 2495.0 73 2495.0 74 1672.0 75 849.0 76 849.0 77 849.0 78 566.0 79 283.0 80 283.0 81 283.0 82 181.5 83 80.0 84 80.0 85 80.0 86 53.5 87 27.0 88 27.0 89 27.0 90 18.5 91 10.0 92 10.0 93 10.0 94 8.0 95 6.0 96 6.0 97 6.0 98 4.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20239227221427367 2 0.13107732229025407 3 0.13463196153880333 4 0.0753139190786375 5 0.023993814927707524 6 0.023993814927707524 7 0.3216948519937083 8 0.31347474873143816 9 0.021327835491295577 10 0.03710154715673293 11 0.01866185605488363 12 0.02066134063219259 13 0.0184396911018493 14 0.020217010726123932 15 0.017995361195780643 16 0.021772165397364235 17 0.021772165397364235 18 0.017328866336677656 19 0.02510463969287917 20 0.02043917567915826 21 0.017773196242746314 22 0.02043917567915826 23 0.021327835491295577 24 0.0184396911018493 25 0.018217526148814972 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 450116.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.27521460065532 #Duplication Level Percentage of deduplicated Percentage of total 1 95.82484100524086 85.54783244816554 2 3.238639624573278 5.782604949959304 3 0.39701061791449344 1.0632962433916553 4 0.16271667431959427 0.5810626407594688 5 0.0849734794781627 0.3793012807888678 6 0.059982997760239874 0.3212996998461626 7 0.03449472002909807 0.21556664732310774 8 0.02638351379650808 0.18843150848820872 9 0.016072183006551638 0.12913628283098127 >10 0.13072935014623752 2.2447757496806418 >50 0.012451461605496587 0.7647855265069674 >100 0.00996116776974411 1.7281038347335156 >500 0.001743204359705078 1.0538031875256029 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 935 0.20772423108709756 No Hit GTCCTACAGTGGACATTTCTAAATT 848 0.18839588017311093 No Hit GTCCTACAGTGTGCATTTCTCATTT 704 0.15640412693616756 No Hit CTGTAGGACGTGGAATATGGCAAGA 646 0.1435185596601765 No Hit GGTATCAACGCAGAGTACTTTTTTT 561 0.12463453865225853 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 528 0.11730309520212567 No Hit GTCCTAAAGTGTGTATTTCTCATTT 518 0.1150814456717824 No Hit CACACACACACACACACACACACAC 494 0.1097494867989585 No Hit CTGTAGGACCTGGAATATGGCGAGA 493 0.10952732184592417 No Hit TATCAACGCAGAGTACTTTTTTTTT 487 0.10819433212771819 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAATACT 50 8.530352E-5 15.233471 4 CTTACTG 40 0.005168113 14.2925415 3 GTAGGAC 425 0.0 13.900196 3 CAGGACC 70 7.072471E-6 13.601314 4 TGTAGGA 470 0.0 13.380252 2 TAGGCTG 50 0.0014732799 13.327801 5 TAAGCTG 50 0.0014732799 13.327801 5 GACGTGG 150 0.0 13.3278 7 TAGGACC 230 0.0 13.246496 4 GTCCTAC 485 0.0 12.979471 1 CTGTAGG 465 0.0 12.71726 1 ACGTGGA 160 0.0 12.485063 8 CCTACAG 505 0.0 12.264227 3 GGACGTG 290 0.0 12.146025 6 ATAAGAG 55 0.002994964 12.127005 3 TCCTACA 545 0.0 11.713766 2 AGGACCT 285 0.0 11.691053 5 TAGGACG 310 0.0 11.670804 4 GTCCTAA 200 0.0 11.445535 1 GTAGAAC 175 1.8189894E-12 11.434033 3 >>END_MODULE