FastQCFastQC Report
Thu 26 May 2016
SRR1926942_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926942_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450116
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15080.335024749175768No Hit
GGTATCAACGCAGAGTACTTTTTTT10640.23638351002852595No Hit
TATCAACGCAGAGTACTTTTTTTTT8610.1912840245625572No Hit
CTGTAGGACGTGGAATATGGCAAGA8530.1895067049382826No Hit
GTCCTACAGTGGACATTTCTAAATT8350.18550773578366464No Hit
GTCCTACAGTGTGCATTTCTCATTT7610.1690675292591243No Hit
CACACACACACACACACACACACAC6890.15307165264065264No Hit
CTGTAGGACCTGGAATATGGCGAGA5920.13152165219632272No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG5830.12952216761901375No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5570.1237458788401212No Hit
GTCCTAAAGTGTGTATTTCTCATTT4980.1106381466110958No Hit
CTTTAGGACGTGAAATATGGCGAGG4940.1097494867989585No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG402.7761413E-416.60886218
TAGGACC1950.015.619734
TCTATAC755.6703357E-815.2309353
GGACCTA456.651461E-414.8062026
TGTAGGA4200.014.7438032
GTCCTAT653.2587795E-614.6581961
GTAGGAC4100.014.395093
CCAACGA400.00530546714.23616819
CTGTAGG4150.014.0047591
CTTCTAG707.0196857E-613.6111831
GTCCTAC3950.013.5078061
CTAGGAC604.012542E-412.6924453
TTAGAGA604.0163173E-412.69103054
CCTGGAC1155.122274E-912.4165223
GGACGTG3300.012.4025986
AGGACGT3650.012.2564065
TAGGACG3650.012.2564064
TCCTACA4650.012.0877222
GGCGAGG1650.012.07917219
CTATACT802.8001201E-511.8978414
Produced by FastQC (version 0.11.5)