##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926942_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 450116 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.828035439753307 31.0 30.0 33.0 26.0 34.0 2 30.128229167592355 31.0 30.0 34.0 26.0 34.0 3 30.185963173937385 31.0 30.0 34.0 26.0 34.0 4 33.748864737089995 35.0 35.0 37.0 28.0 37.0 5 33.70280105572785 37.0 35.0 37.0 28.0 37.0 6 33.64455606999085 37.0 35.0 37.0 29.0 37.0 7 33.64254547716589 37.0 35.0 37.0 28.0 37.0 8 33.67175572519084 37.0 35.0 37.0 29.0 37.0 9 35.180988900638944 39.0 35.0 39.0 29.0 39.0 10 35.08973686782963 39.0 35.0 39.0 29.0 39.0 11 35.10554834753708 39.0 35.0 39.0 29.0 39.0 12 34.99773613912858 39.0 35.0 39.0 28.0 39.0 13 35.01423855183997 39.0 35.0 39.0 28.0 39.0 14 36.09280052253197 40.0 36.0 41.0 28.0 41.0 15 36.086675434776815 40.0 36.0 41.0 28.0 41.0 16 36.06180851158368 40.0 36.0 41.0 27.0 41.0 17 36.06148192910272 40.0 36.0 41.0 28.0 41.0 18 36.03481991308907 40.0 36.0 41.0 27.0 41.0 19 36.11419278585964 40.0 37.0 41.0 28.0 41.0 20 36.072814563357 40.0 37.0 41.0 27.0 41.0 21 36.022985186040934 40.0 36.0 41.0 27.0 41.0 22 35.93707844200162 40.0 36.0 41.0 27.0 41.0 23 35.86455269308356 40.0 36.0 41.0 27.0 41.0 24 35.800524753619065 40.0 36.0 41.0 27.0 41.0 25 35.686269761572575 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.20176684637404918 1101 2 -0.23235190262213123 1101 3 0.0623707464908243 1101 4 -0.15741915555349806 1101 5 -0.3151951396465904 1101 6 -0.19844871493418026 1101 7 -0.13479168124141694 1101 8 -0.18680890350431412 1101 9 -0.10163810176141652 1101 10 -0.09762091809208329 1101 11 0.04968621427173048 1101 12 0.06232523646129806 1101 13 0.2093663223563027 1101 14 -0.1373299934676595 1101 15 -0.19421591929095428 1101 16 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>>Per sequence quality scores pass #Quality Count 2 11448.0 3 7.0 4 164.0 5 1281.0 6 1752.0 7 1346.0 8 1033.0 9 840.0 10 778.0 11 684.0 12 643.0 13 565.0 14 581.0 15 584.0 16 573.0 17 618.0 18 701.0 19 825.0 20 1092.0 21 1464.0 22 1775.0 23 2181.0 24 2669.0 25 3060.0 26 3586.0 27 3990.0 28 4631.0 29 5721.0 30 7242.0 31 9123.0 32 11987.0 33 15881.0 34 21192.0 35 29639.0 36 44085.0 37 75717.0 38 180658.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.890791814946624 19.66725978647687 10.641903914590747 27.800044483985765 2 13.614953520437664 20.846417293065873 41.77022639327492 23.768402793221547 3 18.981868278375256 24.594036085681275 28.74455603946316 27.679539596480314 4 11.156457446524897 17.737872015213856 38.63204151818642 32.473629020074824 5 12.071834681585198 39.217799146391044 35.74892965613468 12.96143651588908 6 31.108019778583305 38.74524596157533 17.994899301329124 12.151834958512236 7 28.275037608353166 31.950323642932855 21.036235120535604 18.738403628178375 8 24.28422245428489 39.86224365098762 19.310773879618726 16.542760015108758 9 25.717942280218008 16.31942515736406 19.94063158923814 38.022000973179786 10 13.540132282009068 31.569276981286336 34.52218289754032 20.368407839164274 11 34.21033337406548 23.61701418589409 23.69988557971095 18.472766860329486 12 22.321061600087088 26.249408481870105 32.29566797670807 19.133861941334732 13 28.84044157996583 22.67773949092557 25.66078145168636 22.821037477422234 14 19.41743906015338 23.874734512881123 28.488434092545035 28.219392334420462 15 22.26221262452567 32.42488469434002 23.39993957005874 21.912963111075566 16 20.501877318877607 30.3314745284486 28.838061807113817 20.328586345559973 17 20.208346292955593 30.068249073572144 28.464440277617324 21.258964355854935 18 19.566284760870094 29.5547098382399 30.771298635225534 20.107706765664467 19 21.952660881465587 28.234180674229197 28.559653776598815 21.2535046677064 20 21.847451301009528 28.868592705815445 29.590635219678656 19.693320773496374 21 21.719007603396864 27.861272446907314 28.326986059698438 22.09273388999738 22 21.222307138604272 29.731002674866037 28.616401105492805 20.430289081036886 23 20.718437025122412 28.96919905091132 28.821459357143492 21.490904566822774 24 21.052128784579974 28.349136666992507 30.174221756169523 20.424512792257996 25 21.170526652565723 28.686834574572085 29.167629441353693 20.975009331508502 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 207.0 1 207.0 2 223.0 3 239.0 4 239.0 5 239.0 6 352.0 7 465.0 8 465.0 9 465.0 10 779.0 11 1093.0 12 1093.0 13 1093.0 14 1760.0 15 2427.0 16 2427.0 17 2427.0 18 4081.5 19 5736.0 20 5736.0 21 5736.0 22 9096.5 23 12457.0 24 12457.0 25 12457.0 26 17594.0 27 22731.0 28 22731.0 29 22731.0 30 29962.0 31 37193.0 32 37193.0 33 37193.0 34 42629.0 35 48065.0 36 48065.0 37 48065.0 38 51914.5 39 55764.0 40 55764.0 41 55764.0 42 58240.0 43 60716.0 44 60716.0 45 60716.0 46 61553.5 47 62391.0 48 62391.0 49 62391.0 50 58733.5 51 55076.0 52 55076.0 53 55076.0 54 46667.5 55 38259.0 56 38259.0 57 38259.0 58 31226.5 59 24194.0 60 24194.0 61 24194.0 62 18696.5 63 13199.0 64 13199.0 65 13199.0 66 9741.5 67 6284.0 68 6284.0 69 6284.0 70 4364.5 71 2445.0 72 2445.0 73 2445.0 74 1627.5 75 810.0 76 810.0 77 810.0 78 550.5 79 291.0 80 291.0 81 291.0 82 172.0 83 53.0 84 53.0 85 53.0 86 33.5 87 14.0 88 14.0 89 14.0 90 10.5 91 7.0 92 7.0 93 7.0 94 3.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11463711576571373 2 0.101307218583654 3 0.016884536430609 4 0.0 5 0.005998453731926881 6 0.3443556772032098 7 0.0184396911018493 8 0.01021958783957913 9 0.009997422886544802 10 0.003776804201583592 11 0.0046654640137209075 12 6.664948591029868E-4 13 0.0011108247651716446 14 0.0 15 0.0017773196242746314 16 0.0013329897182059736 17 0.0 18 0.0011108247651716446 19 4.4432990606865785E-4 20 8.886598121373157E-4 21 0.011996907463853762 22 0.0 23 0.0 24 0.0 25 0.00622061868496121 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 450116.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.13882064960849 #Duplication Level Percentage of deduplicated Percentage of total 1 94.87504493125365 82.67299524388059 2 4.025640881484073 7.01579197542745 3 0.48040996569395733 1.2558707351667093 4 0.18250377015712727 0.6361265318239714 5 0.1090698368289782 0.4752108474861197 6 0.06572058019998371 0.3436088310620755 7 0.04256351079307978 0.2596253893251098 8 0.026890305062838786 0.18745515760671747 9 0.02278606100751664 0.1786995435100527 >10 0.1413111537052269 2.3973002760722695 >50 0.014029502596480637 0.8635396336227008 >100 0.011478682814018497 1.8682239581824034 >500 0.0020406547224921124 1.2738597863584966 >1k 5.101636806230281E-4 0.571692090475319 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1508 0.335024749175768 No Hit GGTATCAACGCAGAGTACTTTTTTT 1064 0.23638351002852595 No Hit TATCAACGCAGAGTACTTTTTTTTT 861 0.1912840245625572 No Hit CTGTAGGACGTGGAATATGGCAAGA 853 0.1895067049382826 No Hit GTCCTACAGTGGACATTTCTAAATT 835 0.18550773578366464 No Hit GTCCTACAGTGTGCATTTCTCATTT 761 0.1690675292591243 No Hit CACACACACACACACACACACACAC 689 0.15307165264065264 No Hit CTGTAGGACCTGGAATATGGCGAGA 592 0.13152165219632272 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 583 0.12952216761901375 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 557 0.1237458788401212 No Hit GTCCTAAAGTGTGTATTTCTCATTT 498 0.1106381466110958 No Hit CTTTAGGACGTGAAATATGGCGAGG 494 0.1097494867989585 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 2.2216495303432893E-4 0.0 0.0 0.0 8 0.0 2.2216495303432893E-4 0.0 0.0 0.0 9 0.0 2.2216495303432893E-4 0.0 0.0 0.0 10 0.0 2.2216495303432893E-4 0.0 0.0 0.0 11 0.0 2.2216495303432893E-4 0.0 0.0 0.0 12 0.0 2.2216495303432893E-4 0.0 0.0 0.0 13 0.0 2.2216495303432893E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 40 2.7761413E-4 16.608862 18 TAGGACC 195 0.0 15.61973 4 TCTATAC 75 5.6703357E-8 15.230935 3 GGACCTA 45 6.651461E-4 14.806202 6 TGTAGGA 420 0.0 14.743803 2 GTCCTAT 65 3.2587795E-6 14.658196 1 GTAGGAC 410 0.0 14.39509 3 CCAACGA 40 0.005305467 14.236168 19 CTGTAGG 415 0.0 14.004759 1 CTTCTAG 70 7.0196857E-6 13.611183 1 GTCCTAC 395 0.0 13.507806 1 CTAGGAC 60 4.012542E-4 12.692445 3 TTAGAGA 60 4.0163173E-4 12.6910305 4 CCTGGAC 115 5.122274E-9 12.416522 3 GGACGTG 330 0.0 12.402598 6 AGGACGT 365 0.0 12.256406 5 TAGGACG 365 0.0 12.256406 4 TCCTACA 465 0.0 12.087722 2 GGCGAGG 165 0.0 12.079172 19 CTATACT 80 2.8001201E-5 11.897841 4 >>END_MODULE