FastQCFastQC Report
Thu 26 May 2016
SRR1926941_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926941_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences626800
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT9940.1585832801531589No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGACG307.5551204E-419.0594883
TTACTCT508.559011E-515.2292774
AATCCCG456.836429E-414.75425619
GTATTAG706.9692796E-613.6226581
GGTGAGC500.001488755113.3106958
GGCGAGG1102.7976057E-912.93392519
GTCTTAG1051.8830178E-812.714481
GTAAGAC751.4285621E-512.70632653
CTAGTAC603.979577E-412.7063263
GGACGTG2000.012.3708156
GACGTGA853.85931E-612.3148397
GTAGGAC3950.012.3042263
GTCCTAG701.05124534E-412.2603921
CTAGATT701.0695015E-412.2378114
TAGGACG2350.012.1510194
ATAGGAC550.002993977912.1287653
CCTAGAT550.002993977912.1287653
TGGCGAG2900.012.101397518
GCAACTT550.00310171612.07166313
TGCGCCA550.003105425712.06973712
Produced by FastQC (version 0.11.5)