##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926941_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 626800 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.25215220165922 31.0 28.0 31.0 16.0 34.0 2 28.233203573707723 31.0 28.0 31.0 16.0 34.0 3 27.936402361199743 31.0 28.0 31.0 16.0 34.0 4 31.842613273771537 35.0 32.0 37.0 19.0 37.0 5 31.86971761327377 35.0 32.0 37.0 19.0 37.0 6 31.38442884492661 35.0 32.0 37.0 17.0 37.0 7 31.261490108487557 35.0 32.0 37.0 17.0 37.0 8 30.954993618379067 35.0 32.0 37.0 11.0 37.0 9 33.129709636247604 37.0 33.0 39.0 17.0 39.0 10 33.30798500319081 37.0 33.0 39.0 17.0 39.0 11 33.44956764518188 38.0 34.0 39.0 18.0 39.0 12 33.338608806636884 38.0 34.0 39.0 17.0 39.0 13 33.42805360561583 38.0 34.0 39.0 18.0 39.0 14 34.44363273771538 39.0 34.0 41.0 17.0 41.0 15 34.02433471601787 39.0 33.0 41.0 15.0 41.0 16 34.10115985960434 39.0 33.0 41.0 17.0 41.0 17 34.331963943841735 39.0 34.0 41.0 17.0 41.0 18 34.38081684747926 39.0 34.0 41.0 17.0 41.0 19 33.78074664964901 39.0 33.0 41.0 10.0 41.0 20 34.05977345245692 39.0 34.0 41.0 11.0 41.0 21 34.03396777281429 39.0 34.0 41.0 10.0 41.0 22 34.05786375239311 39.0 34.0 41.0 10.0 41.0 23 34.03913369495852 39.0 34.0 41.0 2.0 41.0 24 32.7701356094448 39.0 32.0 41.0 2.0 41.0 25 33.33461391193363 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.5709955708162902 1101 2 -0.6499818935677837 1101 3 -1.1676240214486953 1101 4 -0.7688705117248489 1101 5 -0.5180462014148262 1101 6 -2.39101323759672 1101 7 -0.8794677579427166 1101 8 -0.4968082560013585 1101 9 -0.5447283279404544 1101 10 -9.917276530427895 1101 11 -4.480476124996471 1101 12 -1.9724877243304029 1101 13 -1.151015664599754 1101 14 -0.9885762757531111 1101 15 -1.8897478600172306 1101 16 -0.7380070645862062 1101 17 -0.3924268311896739 1101 18 -0.4454045193300402 1101 19 -1.1676853297800776 1101 20 -0.604747039140328 1101 21 -0.658413727647428 1101 22 -0.3110453099806989 1101 23 -0.43485795305534225 1101 24 -0.4416177116295472 1101 25 -0.27612852148100586 1102 1 -0.32772801883491454 1102 2 -0.386489341466163 1102 3 -0.48089989429813684 1102 4 -0.5691480343186512 1102 5 -0.14529055116063105 1102 6 -0.7225685665595911 1102 7 -0.21301913329082112 1102 8 0.3582523228039953 1102 9 0.010076372147565849 1102 10 0.09651783350667387 1102 11 -0.14932481506097162 1102 12 0.03402708248597719 1102 13 -0.309900956864233 1102 14 -0.4088931421045743 1102 15 0.04018369723563353 1102 16 -0.10127548232264161 1102 17 -0.004974988814808512 1102 18 -0.19687029074184892 1102 19 0.06319951998737139 1102 20 0.04848920136642931 1102 21 -0.01474167566364315 1102 22 -0.21209672140193447 1102 23 -0.4496599596831743 1102 24 -3.82813646244119 1102 25 -2.1934368890036495 1103 1 -0.5010957896305257 1103 2 -0.7040200108181125 1103 3 -0.0853887061572749 1103 4 -0.3263542427443724 1103 5 -0.4253958968746687 1103 6 0.2887696532140289 1103 7 -0.012501603105594228 1103 8 0.07030898485533044 1103 9 -0.22253383902798873 1103 10 -0.1935508154926353 1103 11 -0.3815574083277511 1103 12 -0.14927494406347108 1103 13 -0.24882610137570538 1103 14 -0.05804979544165434 1103 15 0.15702013058606923 1103 16 0.19780032205191844 1103 17 -0.27065339391167953 1103 18 -0.5049532504916527 1103 19 -1.6792820106238864 1103 20 -0.6705049794447717 1103 21 -0.4364948878364956 1103 22 -0.3860720697302469 1103 23 -0.3306062314325189 1103 24 0.8927796967005506 1103 25 0.12766662671590723 1104 1 -0.36682103963525137 1104 2 -0.23890724876212133 1104 3 -1.2990553161120921 1104 4 -0.3209429873331153 1104 5 -0.09231304086604553 1104 6 -1.5370021005053225 1104 7 -0.17244791589033426 1104 8 -0.04099274107521467 1104 9 0.14461865912887362 1104 10 0.1794288249542504 1104 11 0.2739937363063589 1104 12 0.10469015924661562 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-3.2220030764776517 2216 11 -1.8870119246993013 2216 12 -1.8208842663278126 2216 13 -1.2973768955596867 2216 14 -1.5938240648403763 2216 15 -2.7604303228905813 2216 16 -1.6284109484824967 2216 17 -1.9575305633216047 2216 18 -1.6097927341520943 2216 19 -0.9750975487975069 2216 20 -2.050133720427226 2216 21 -4.394250378343077 2216 22 -3.038278669535103 2216 23 -3.024613461465947 2216 24 -3.2081568485591383 2216 25 -2.6071694978582087 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35415.0 3 2.0 4 282.0 5 1868.0 6 2259.0 7 1918.0 8 1472.0 9 1253.0 10 1226.0 11 1210.0 12 1144.0 13 1123.0 14 1140.0 15 1199.0 16 1234.0 17 1415.0 18 1531.0 19 1982.0 20 2523.0 21 3255.0 22 4178.0 23 4955.0 24 5693.0 25 6600.0 26 7652.0 27 8033.0 28 9058.0 29 10927.0 30 12974.0 31 15579.0 32 20244.0 33 26843.0 34 35457.0 35 49710.0 36 71445.0 37 114051.0 38 159950.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.93284213320286 19.130102477266803 13.002093037014294 24.93496235251604 2 13.73548601481273 20.358879651344843 43.23601960546243 22.669614728379994 3 18.920287701741103 24.88073670050453 29.105996555503367 27.092979042251002 4 10.931834691743369 17.099681485443327 40.26918072308393 31.69930309972938 5 12.142382964598946 38.27140595677318 36.961420127408054 12.624790951219826 6 30.89647292740802 37.83668200176489 19.237808916059087 12.029036154768002 7 27.345548925575137 32.27963049193601 22.518357791720472 17.85646279076838 8 23.840918655876898 37.954403252932806 21.137182295775574 17.06749579541472 9 25.57150562170684 16.487893304057266 20.727391100373715 37.21320997386218 10 13.34146220791512 30.57616590564866 35.49003867067365 20.59233321576257 11 33.47784876781577 23.910310008744325 24.168331492982198 18.443509730457706 12 22.053398182189543 26.04198580473856 32.503733915441174 19.40088209763072 13 27.91900366206847 22.810138903294266 26.224558996002838 23.046298438634423 14 19.7377302400097 23.113599698731257 29.023286095202838 28.125383966056205 15 22.515577753133503 31.348582643869825 24.01088248669608 22.124957116300592 16 20.877554960880715 30.08666401775727 28.315245750175926 20.720535271186087 17 20.667293248079964 29.688150749743492 28.33945819324345 21.305097808933095 18 20.279242693814375 29.343460535029003 29.925083174430945 20.45221359672568 19 22.30573441526837 28.36249611029993 28.536435518746362 20.795333955685344 20 21.962868107581958 28.573206634912275 29.39882077917933 20.065104478326433 21 22.242061592468485 27.887665549704803 28.26280516993777 21.607467687888942 22 21.470652062908496 28.988906760620914 28.800296160130717 20.74014501633987 23 21.288719673711153 28.676813194528663 28.42038411703506 21.614083014725125 24 21.57146527224828 28.335426343848386 29.19757646934923 20.89553191455411 25 21.550480615800964 28.4050001914804 28.788758824055044 21.255760368663594 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 148.0 1 148.0 2 212.5 3 277.0 4 277.0 5 277.0 6 452.0 7 627.0 8 627.0 9 627.0 10 981.5 11 1336.0 12 1336.0 13 1336.0 14 2348.5 15 3361.0 16 3361.0 17 3361.0 18 6050.5 19 8740.0 20 8740.0 21 8740.0 22 13690.0 23 18640.0 24 18640.0 25 18640.0 26 25871.0 27 33102.0 28 33102.0 29 33102.0 30 42556.0 31 52010.0 32 52010.0 33 52010.0 34 59718.0 35 67426.0 36 67426.0 37 67426.0 38 72773.0 39 78120.0 40 78120.0 41 78120.0 42 81411.0 43 84702.0 44 84702.0 45 84702.0 46 84925.5 47 85149.0 48 85149.0 49 85149.0 50 79678.5 51 74208.0 52 74208.0 53 74208.0 54 63487.5 55 52767.0 56 52767.0 57 52767.0 58 43173.5 59 33580.0 60 33580.0 61 33580.0 62 26003.0 63 18426.0 64 18426.0 65 18426.0 66 13544.0 67 8662.0 68 8662.0 69 8662.0 70 6150.0 71 3638.0 72 3638.0 73 3638.0 74 2475.0 75 1312.0 76 1312.0 77 1312.0 78 858.5 79 405.0 80 405.0 81 405.0 82 258.5 83 112.0 84 112.0 85 112.0 86 76.0 87 40.0 88 40.0 89 40.0 90 22.5 91 5.0 92 5.0 93 5.0 94 4.5 95 4.0 96 4.0 97 4.0 98 3.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22223994894703253 2 0.13592852584556478 3 0.13943841735800894 4 0.07259093809827696 5 0.024250159540523293 6 0.021218889597957882 7 0.2969049138481174 8 0.3010529674537332 9 0.0194639438417358 10 0.03621569878749203 11 0.01723037651563497 12 0.017868538608806637 13 0.01675175494575622 14 0.01866624122527122 15 0.01675175494575622 16 0.020261646458200385 17 0.018985322271857053 18 0.01675175494575622 19 0.023133375877472877 20 0.018347160178685384 21 0.015954052329291642 22 0.017868538608806637 23 0.018506700701978303 24 0.017708998085513718 25 0.016592214422463305 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 626800.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.32501356850749 #Duplication Level Percentage of deduplicated Percentage of total 1 95.40570149036009 86.17521281629749 2 3.6712191951273896 6.632058472256931 3 0.43115335174899105 1.168317970405053 4 0.14734977044908573 0.5323748006052053 5 0.08439701301106427 0.38115806726829415 6 0.05503621657448118 0.2982688205309597 7 0.038589012907132327 0.24398871801024263 8 0.026626350267220336 0.1924020359333208 9 0.019017658439631793 0.15459932309408841 >10 0.10322534955202803 1.742686537347108 >50 0.009903430644020345 0.6210560666445103 >100 0.006543248955290877 1.1574389296393095 >500 0.0012379119636056024 0.7004374419675046 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 994 0.1585832801531589 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.595405232929164E-4 0.0 5 0.0 0.0 0.0 1.595405232929164E-4 0.0 6 0.0 0.0 0.0 1.595405232929164E-4 0.0 7 0.0 0.0 0.0 1.595405232929164E-4 0.0 8 0.0 0.0 0.0 1.595405232929164E-4 0.0 9 0.0 0.0 0.0 1.595405232929164E-4 0.0 10 0.0 0.0 0.0 1.595405232929164E-4 1.595405232929164E-4 11 0.0 0.0 0.0 1.595405232929164E-4 1.595405232929164E-4 12 0.0 0.0 0.0 3.190810465858328E-4 1.595405232929164E-4 13 0.0 0.0 0.0 3.190810465858328E-4 1.595405232929164E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGACG 30 7.5551204E-4 19.059488 3 TTACTCT 50 8.559011E-5 15.229277 4 AATCCCG 45 6.836429E-4 14.754256 19 GTATTAG 70 6.9692796E-6 13.622658 1 GGTGAGC 50 0.0014887551 13.310695 8 GGCGAGG 110 2.7976057E-9 12.933925 19 GTCTTAG 105 1.8830178E-8 12.71448 1 GTAAGAC 75 1.4285621E-5 12.7063265 3 CTAGTAC 60 3.979577E-4 12.706326 3 GGACGTG 200 0.0 12.370815 6 GACGTGA 85 3.85931E-6 12.314839 7 GTAGGAC 395 0.0 12.304226 3 GTCCTAG 70 1.05124534E-4 12.260392 1 CTAGATT 70 1.0695015E-4 12.237811 4 TAGGACG 235 0.0 12.151019 4 ATAGGAC 55 0.0029939779 12.128765 3 CCTAGAT 55 0.0029939779 12.128765 3 TGGCGAG 290 0.0 12.1013975 18 GCAACTT 55 0.003101716 12.071663 13 TGCGCCA 55 0.0031054257 12.069737 12 >>END_MODULE