FastQCFastQC Report
Thu 26 May 2016
SRR1926941_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926941_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences626800
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16280.2597319719208679No Hit
GGTATCAACGCAGAGTACTTTTTTT10110.16129546904913847No Hit
TATCAACGCAGAGTACTTTTTTTTT8650.1380025526483727No Hit
CACACACACACACACACACACACAC8240.13146139119336311No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG6740.10753031269942566No Hit
GTACATGGGAAGCAGTGGTATCAAC6380.10178685386088066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2150.013.7331874
GTAGGAC3950.013.0230993
GTGTAAG1102.582965E-913.0037561
AGGACGT2900.012.8089855
TAAGACC751.4364767E-512.6995054
GACAGTT604.129214E-412.6508687
TAGGACG3000.012.3820174
GGACGTG3050.012.1790356
TCAGTAC550.003002440312.1241983
TAATACT951.0045442E-612.0311114
TTAGACT657.839477E-411.7226214
CTGTAGG4000.011.6817071
AGGACCT3300.011.5450055
GACGTGA1900.011.4856577
TGTAGGA4450.011.3575872
CTAAGAC855.164126E-511.2072423
TAAGGAC600.005750090311.1138493
GACGTGG1553.1650416E-1011.0184987
GGCGAGG1658.185452E-1110.924005519
TGGCGAG4000.010.90963418
Produced by FastQC (version 0.11.5)