##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926941_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 626800 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.00426930440332 31.0 30.0 33.0 26.0 34.0 2 30.323237077217613 31.0 30.0 34.0 26.0 34.0 3 30.3763225909381 31.0 30.0 34.0 26.0 34.0 4 33.969484684109766 37.0 35.0 37.0 30.0 37.0 5 33.93834716017869 37.0 35.0 37.0 30.0 37.0 6 33.89734524569241 37.0 35.0 37.0 30.0 37.0 7 33.900376515634974 37.0 35.0 37.0 30.0 37.0 8 33.91108008934269 37.0 35.0 37.0 30.0 37.0 9 35.472584556477344 39.0 35.0 39.0 30.0 39.0 10 35.36257338864071 39.0 35.0 39.0 30.0 39.0 11 35.391707083599236 39.0 35.0 39.0 30.0 39.0 12 35.29339821314614 39.0 35.0 39.0 30.0 39.0 13 35.30804243777919 39.0 35.0 39.0 30.0 39.0 14 36.430221761327374 40.0 37.0 41.0 30.0 41.0 15 36.40847160178686 40.0 37.0 41.0 30.0 41.0 16 36.38065092533503 40.0 36.0 41.0 30.0 41.0 17 36.36657626037013 40.0 36.0 41.0 30.0 41.0 18 36.34006700701978 40.0 36.0 41.0 30.0 41.0 19 36.42111837906828 40.0 37.0 41.0 30.0 41.0 20 36.38100829610721 40.0 37.0 41.0 30.0 41.0 21 36.336566687938735 40.0 37.0 41.0 30.0 41.0 22 36.2489534141672 40.0 37.0 41.0 29.0 41.0 23 36.186097638800256 40.0 37.0 41.0 29.0 41.0 24 36.12283024888322 40.0 37.0 41.0 29.0 41.0 25 36.023771537970646 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3154100891282283 1101 2 -0.40564445040591224 1101 3 -0.06078368291923297 1101 4 -0.35739530958016275 1101 5 -0.4707523809033418 1101 6 -0.5900926799163528 1101 7 -0.4774779363547026 1101 8 -0.6320785151891286 1101 9 -0.675228645675908 1101 10 -0.4632505929513897 1101 11 -0.5633128823016733 1101 12 -0.5500853379418018 1101 13 -0.5077051537090469 1101 14 -0.5399629770192931 1101 15 -0.5703237115808832 1101 16 -0.47684806430532944 1101 17 -0.48471910805716334 1101 18 -0.4963272187670924 1101 19 -0.6476773249803855 1101 20 -0.6755388538181464 1101 21 -0.5143864658754467 1101 22 -0.5419603483745448 1101 23 -0.3079040874368175 1101 24 -0.4990951939036279 1101 25 -0.5347202577668781 1102 1 -0.11337893156561663 1102 2 -0.19600907757811825 1102 3 -0.04698931898466441 1102 4 -0.03326623512041493 1102 5 -0.03449840684713479 1102 6 0.045003587236422504 1102 7 -0.02365153948296239 1102 8 -0.14346800396076986 1102 9 0.1850192488558875 1102 10 0.11072096218518368 1102 11 0.053946425767641415 1102 12 0.15008685111986608 1102 13 0.035373375084525094 1102 14 0.05034970484938839 1102 15 0.14015817730426505 1102 16 -0.01371720542821464 1102 17 0.014371849131251224 1102 18 -0.2361726333916181 1102 19 -0.061890618168341405 1102 20 0.020441637834530013 1102 21 0.07981008938136824 1102 22 -0.032809798379595634 1102 23 -0.11176246914822485 1102 24 -0.08873210585153402 1102 25 -0.003918616680060438 1103 1 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>>Per sequence quality scores pass #Quality Count 2 13688.0 3 11.0 4 229.0 5 1611.0 6 2020.0 7 1503.0 8 1237.0 9 1052.0 10 959.0 11 893.0 12 755.0 13 759.0 14 740.0 15 746.0 16 789.0 17 787.0 18 868.0 19 1051.0 20 1458.0 21 1802.0 22 2237.0 23 2758.0 24 3227.0 25 4024.0 26 4891.0 27 5395.0 28 6212.0 29 7542.0 30 9800.0 31 12825.0 32 16619.0 33 21766.0 34 29519.0 35 41582.0 36 61380.0 37 106095.0 38 257970.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.53394488378891 19.86170632480325 11.188242641667957 27.416106149739882 2 13.517368270583743 21.016174396634472 41.55272291104002 23.91373442174176 3 18.446679399849053 25.069051186435807 29.194410803100002 27.289858610615138 4 11.322590938098278 18.02409061901723 38.82275047862157 31.830567964262922 5 12.38628132349234 38.93781730342744 35.67168608380161 13.004215289278614 6 30.528053333674887 38.85695713709342 18.43319521738852 12.181794311843175 7 27.99222562289165 32.18834673211271 21.48788684387736 18.331540801118283 8 24.462736600924465 38.63828918165558 20.031400530681733 16.867573686738222 9 25.616846646753977 16.528490464884737 20.39503076255393 37.45963212580736 10 13.506877246612431 31.165629881121994 34.653951884003405 20.673540988262175 11 33.543617136694046 23.999476683706355 23.92752069333027 18.52938548626933 12 22.09359649136802 26.3436168328821 32.09859013364774 19.464196542102147 13 28.033408052074442 22.780334878229723 26.157994240541164 23.028262829154666 14 19.995373324824506 23.392469687300572 28.512284620293553 28.099872367581362 15 22.41459908516399 31.363526857153794 23.884814264677022 22.33705979300519 16 21.025308034339666 30.257005059054208 27.870905572298525 20.8467813343076 17 20.583280153158903 29.810625398851307 28.210274409700066 21.395820038289727 18 20.24527838314736 29.472653071643496 29.720579835928003 20.56148870928114 19 21.855775366943202 28.551212507977024 28.635928525845568 20.957083599234206 20 21.903014542234704 28.510438022000812 29.39701178216163 20.189535653602853 21 22.12407172787066 27.834760380531737 28.28457544415049 21.75659244744711 22 21.34301212507977 29.04834077855775 28.666241225271214 20.942405871091257 23 21.299617102744097 28.561901723037654 28.444639438417358 21.69384173580089 24 21.07051691129547 28.421186981493303 29.555520102105937 20.952776005105296 25 21.547322739166507 28.299508583827944 28.807039377114048 21.346129299891505 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 234.0 1 234.0 2 227.5 3 221.0 4 221.0 5 221.0 6 373.5 7 526.0 8 526.0 9 526.0 10 922.0 11 1318.0 12 1318.0 13 1318.0 14 2248.0 15 3178.0 16 3178.0 17 3178.0 18 5700.0 19 8222.0 20 8222.0 21 8222.0 22 12911.0 23 17600.0 24 17600.0 25 17600.0 26 24952.5 27 32305.0 28 32305.0 29 32305.0 30 41609.5 31 50914.0 32 50914.0 33 50914.0 34 58475.0 35 66036.0 36 66036.0 37 66036.0 38 71700.5 39 77365.0 40 77365.0 41 77365.0 42 80800.5 43 84236.0 44 84236.0 45 84236.0 46 85435.5 47 86635.0 48 86635.0 49 86635.0 50 81696.5 51 76758.0 52 76758.0 53 76758.0 54 65191.5 55 53625.0 56 53625.0 57 53625.0 58 43870.5 59 34116.0 60 34116.0 61 34116.0 62 26492.0 63 18868.0 64 18868.0 65 18868.0 66 13913.0 67 8958.0 68 8958.0 69 8958.0 70 6303.0 71 3648.0 72 3648.0 73 3648.0 74 2514.0 75 1380.0 76 1380.0 77 1380.0 78 916.5 79 453.0 80 453.0 81 453.0 82 298.0 83 143.0 84 143.0 85 143.0 86 90.5 87 38.0 88 38.0 89 38.0 90 28.5 91 19.0 92 19.0 93 19.0 94 11.5 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11821952776005105 2 0.10960433950223357 3 0.014518187619655393 4 0.0 5 0.005424377791959158 6 0.35162731333758773 7 0.020102105934907466 8 0.009412890874282067 9 0.010210593490746649 10 0.0036694320357370767 11 0.004467134652201659 12 4.7862156987874915E-4 13 7.97702616464582E-4 14 0.0 15 0.003350350989151244 16 4.7862156987874915E-4 17 0.0 18 3.1908104658583277E-4 19 0.0 20 7.97702616464582E-4 21 0.015634971282705808 22 0.0 23 0.0 24 0.0 25 0.006381620931716656 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 626800.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.03771169835933 #Duplication Level Percentage of deduplicated Percentage of total 1 94.09724603381105 82.84106217934243 2 4.7314893491064325 8.3309899044098 3 0.5880109378626536 1.5530141226910255 4 0.1742334552294889 0.6135645879881098 5 0.10019559155781951 0.44104953015069404 6 0.06490971281713513 0.3428701550050946 7 0.04154807459128173 0.25604581887424327 8 0.03486270392252479 0.24553861415651987 9 0.024748528772830472 0.19609234569549 >10 0.12136405749685596 1.9471201017504043 >50 0.011783558671009706 0.7119719724539177 >100 0.007795166697381686 1.246455363801544 >500 0.0014502635708866693 0.8530478767752382 >1k 3.6256589272166734E-4 0.42117742690549176 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1628 0.2597319719208679 No Hit GGTATCAACGCAGAGTACTTTTTTT 1011 0.16129546904913847 No Hit TATCAACGCAGAGTACTTTTTTTTT 865 0.1380025526483727 No Hit CACACACACACACACACACACACAC 824 0.13146139119336311 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 674 0.10753031269942566 No Hit GTACATGGGAAGCAGTGGTATCAAC 638 0.10178685386088066 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 3.190810465858328E-4 0.0 13 0.0 0.0 0.0 3.190810465858328E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 215 0.0 13.733187 4 GTAGGAC 395 0.0 13.023099 3 GTGTAAG 110 2.582965E-9 13.003756 1 AGGACGT 290 0.0 12.808985 5 TAAGACC 75 1.4364767E-5 12.699505 4 GACAGTT 60 4.129214E-4 12.650868 7 TAGGACG 300 0.0 12.382017 4 GGACGTG 305 0.0 12.179035 6 TCAGTAC 55 0.0030024403 12.124198 3 TAATACT 95 1.0045442E-6 12.031111 4 TTAGACT 65 7.839477E-4 11.722621 4 CTGTAGG 400 0.0 11.681707 1 AGGACCT 330 0.0 11.545005 5 GACGTGA 190 0.0 11.485657 7 TGTAGGA 445 0.0 11.357587 2 CTAAGAC 85 5.164126E-5 11.207242 3 TAAGGAC 60 0.0057500903 11.113849 3 GACGTGG 155 3.1650416E-10 11.018498 7 GGCGAGG 165 8.185452E-11 10.9240055 19 TGGCGAG 400 0.0 10.909634 18 >>END_MODULE