FastQCFastQC Report
Thu 26 May 2016
SRR1926940_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926940_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461524
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT8460.18330574357996549No Hit
GGTATCAACGCAGAGTACTTTTTTT5060.10963676861875005No Hit
CACACACACACACACACACACACAC4800.10400325876877477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC1250.016.0022564
CTCCTAG456.469418E-414.8605921
GTCTTAA602.4290142E-514.3298571
TAACACT551.9120518E-413.8547684
TGTAGGA2700.013.0729142
GTAGGAC2300.012.8578483
GTCTTAT603.8942308E-412.737651
TAGGACT751.433708E-512.7002034
TATGCTG751.4433734E-512.6919115
GGGTTTC550.00293720412.1586671
CTGTAGG2550.011.9883781
ATATAGC657.729325E-411.7411623
AGTAGGA657.729325E-411.7411622
GTCCTAC2450.011.6978431
TAGACAG907.265675E-611.6342525
GGCGAGA1406.9667294E-1011.51110719
GTCTAGA751.9631273E-411.4638851
GTACTGA751.9631273E-411.4638851
CCTGGAC751.9891861E-411.4476333
AACTGTC752.0298737E-411.422727
Produced by FastQC (version 0.11.5)