FastQCFastQC Report
Thu 26 May 2016
SRR1926940_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926940_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461524
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13410.2905591041852644No Hit
GGTATCAACGCAGAGTACTTTTTTT8960.19413941636837953No Hit
TATCAACGCAGAGTACTTTTTTTTT7000.15167141903779652No Hit
CACACACACACACACACACACACAC6810.1475546233781992No Hit
GTACATGGGAAGCAGTGGTATCAAC5570.12068711486293236No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG5550.12025376795139582No Hit
ACGCAGAGTACTTTTTTTTTTTTTT4760.10313656494570164No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG307.779259E-418.97529618
GACGTGG1050.016.26637
ACGTGGA1001.8189894E-1216.1307498
CGTGGAA1055.456968E-1215.3642839
TCCAATT400.005317360614.23147112
GTAGGAC2750.014.1975243
CCTGTAC1101.7826096E-1013.8512433
ACTGATC551.9753381E-413.801718
GTCCTAC2800.013.623161
TAGGACC1351.8189894E-1213.4009034
GTGTTAA500.001454437313.3506971
GGCCAGA500.001454437313.3506971
AGTGCCA500.001511509213.28270610
TAGGACG2050.013.0052414
GGACGTG1850.012.8657566
ACTGTTC1052.0128937E-812.6515678
AGGACGT1900.012.5285445
CCCTGTA1155.007678E-912.4358282
TGTAGGA3300.012.4232672
TCCTACA3850.012.1343552
Produced by FastQC (version 0.11.5)