##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926940_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 461524 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.940967316975932 31.0 30.0 33.0 26.0 34.0 2 30.24471750114837 31.0 30.0 34.0 26.0 34.0 3 30.29393270989158 31.0 30.0 34.0 26.0 34.0 4 33.87160797704995 35.0 35.0 37.0 29.0 37.0 5 33.83747324082821 37.0 35.0 37.0 30.0 37.0 6 33.797083141938444 37.0 35.0 37.0 30.0 37.0 7 33.78723966684289 37.0 35.0 37.0 29.0 37.0 8 33.81569105831983 37.0 35.0 37.0 30.0 37.0 9 35.362639862715696 39.0 35.0 39.0 30.0 39.0 10 35.26161369722918 39.0 35.0 39.0 30.0 39.0 11 35.283369878922876 39.0 35.0 39.0 30.0 39.0 12 35.17337559910211 39.0 35.0 39.0 29.0 39.0 13 35.19422175228157 39.0 35.0 39.0 29.0 39.0 14 36.29419921824217 40.0 36.0 41.0 29.0 41.0 15 36.28364072074258 40.0 36.0 41.0 29.0 41.0 16 36.25611452492178 40.0 36.0 41.0 29.0 41.0 17 36.246442221856285 40.0 36.0 41.0 29.0 41.0 18 36.2127148317314 40.0 36.0 41.0 29.0 41.0 19 36.303459841741706 40.0 37.0 41.0 29.0 41.0 20 36.25719789220062 40.0 37.0 41.0 29.0 41.0 21 36.21780882467651 40.0 37.0 41.0 29.0 41.0 22 36.1253585945693 40.0 37.0 41.0 29.0 41.0 23 36.06256879382221 40.0 37.0 41.0 29.0 41.0 24 35.991365562787635 40.0 37.0 41.0 28.0 41.0 25 35.882155640876746 40.0 36.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.23402194068930626 1101 2 -0.17696503870478608 1101 3 0.009576011705785703 1101 4 0.04697029271986963 1101 5 -0.06339320489529143 1101 6 -0.3342041517878158 1101 7 -0.37199843417850786 1101 8 0.04103905499047755 1101 9 -0.08430505802159871 1101 10 -0.00972873402732688 1101 11 -0.014965565791243307 1101 12 -0.12852526006604847 1101 13 -0.3904748671324043 1101 14 -0.19201217516933156 1101 15 -0.3313652103218345 1101 16 -0.11052838294652645 1101 17 -0.15661887542069763 1101 18 0.002662875011182564 1101 19 0.051054684623913715 1101 20 -0.0750776780405289 1101 21 -0.09630845049506576 1101 22 -0.2998665308014097 1101 23 -0.6200314706713073 1101 24 -0.4560725676369515 1101 25 -0.36836003204847145 1102 1 -0.48581250788488717 1102 2 -0.2985395105203388 1102 3 -0.2800904211862978 1102 4 -0.5938730490877973 1102 5 -0.6299466369983833 1102 6 -0.6219770105587514 1102 7 -0.6978378310400828 1102 8 -0.6064953537059381 1102 9 -0.5905402644737805 1102 10 -0.6474270771499704 1102 11 -0.5232631023915744 1102 12 -0.7366715737330978 1102 13 -0.7273820294011912 1102 14 -0.6202952469673519 1102 15 -0.6903027048427433 1102 16 -0.5919498785194222 1102 17 -0.7340910416344215 1102 18 -0.7428508446346456 1102 19 -0.7466703964666479 1102 20 -0.7215491429305061 1102 21 -0.5763974309456614 1102 22 -0.6468902881433962 1102 23 -0.5936441646311579 1102 24 -0.6562829647615231 1102 25 -0.6896999987355912 1103 1 -0.4439920660785184 1103 2 -0.6260267234645269 1103 3 -0.34458749919213716 1103 4 -0.6509931845165227 1103 5 -0.47890906789285026 1103 6 -0.411804505233917 1103 7 -0.19760223793176834 1103 8 -0.16188318686764092 1103 9 -0.3902989147917779 1103 10 -0.4810343807495343 1103 11 -0.2754511334088434 1103 12 -0.3128747509353573 1103 13 -0.3127083098657266 1103 14 -0.3755790353898192 1103 15 -0.18145357184670274 1103 16 -0.15779548721312864 1103 17 -0.26690457768443565 1103 18 -0.3188194947607599 1103 19 -0.45789507553766384 1103 20 -0.4879111376046126 1103 21 -0.416503266618939 1103 22 -0.5131544210648116 1103 23 -0.2277904540930038 1103 24 -0.2770100413388761 1103 25 -0.29718482376772926 1104 1 -0.4466213047592582 1104 2 -0.3008375179952232 1104 3 -0.025603052807642968 1104 4 -0.34482118916449167 1104 5 -0.43961904373722405 1104 6 -0.4266336555950332 1104 7 -0.7455278459432151 1104 8 -0.609285185658841 1104 9 -1.0102212993403157 1104 10 -0.4298965024149126 1104 11 -0.6692162561100403 1104 12 -0.4938607968412114 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2212 23 0.1410270424436959 2212 24 0.06981599359229307 2212 25 0.002395050987381353 2213 1 -0.17028155682109514 2213 2 -0.11834354519324819 2213 3 -0.16946470629872223 2213 4 -0.10018177814811224 2213 5 -0.09053012307074937 2213 6 -0.132624774990866 2213 7 -0.42584488689433897 2213 8 -0.4572494678162684 2213 9 -0.4579063479431156 2213 10 -0.21184816832681008 2213 11 -0.36177184444537147 2213 12 -0.3153599014523252 2213 13 -0.24428635209627458 2213 14 -0.24263576674599108 2213 15 -0.2861247098944517 2213 16 -0.26675847075443926 2213 17 -0.3011580781559502 2213 18 -0.4848672361085633 2213 19 -0.5094822646339949 2213 20 -0.2381128789108331 2213 21 -0.26190989582881485 2213 22 -0.33836991101743763 2213 23 -0.25504584630267146 2213 24 -0.2907605775833346 2213 25 -0.2725619902425791 2214 1 0.26694722560316464 2214 2 0.35867333878361407 2214 3 0.4561147035183879 2214 4 0.4322718942765178 2214 5 0.6025213288571436 2214 6 0.47497486332967753 2214 7 0.3880599403163245 2214 8 0.4285895713802361 2214 9 0.3809883112345034 2214 10 0.09995918694258421 2214 11 0.34284763582528655 2214 12 0.16127042920202683 2214 13 0.09466208841317325 2214 14 0.053119541639524925 2214 15 0.14824861994309657 2214 16 0.2719405588286534 2214 17 0.1091833996878222 2214 18 0.010636980683983666 2214 19 0.06745193054518239 2214 20 0.28835593968137374 2214 21 0.10963003176807007 2214 22 0.13247892646546688 2214 23 0.2213844850239468 2214 24 0.30490632773799575 2214 25 0.07744523662388758 2215 1 0.5907666506614824 2215 2 0.45189038745054333 2215 3 0.3348356363627083 2215 4 0.3113201184004524 2215 5 0.5596670280251175 2215 6 0.5850618522178195 2215 7 0.2721266307276551 2215 8 0.32953582822688077 2215 9 0.10979218995704088 2215 10 0.3705713178976069 2215 11 0.07675982550020422 2215 12 0.11085794592757026 2215 13 0.10501508168444929 2215 14 0.02638594069744471 2215 15 0.059095437998024636 2215 16 0.14781854977359643 2215 17 0.12524634229312426 2215 18 0.03784387048629867 2215 19 0.11255234800199787 2215 20 -0.06661391422478857 2215 21 0.1750642221553349 2215 22 0.13552401886107646 2215 23 0.1119617049091346 2215 24 0.16121101666688276 2215 25 0.22179833899225798 2216 1 -0.3969661808853857 2216 2 0.03509072109913447 2216 3 -1.6789043804510762 2216 4 -1.7097545847311153 2216 5 -0.546343572542348 2216 6 -0.15735059786624817 2216 7 -0.17391326260987938 2216 8 -0.025857391087953374 2216 9 -0.18280383253140542 2216 10 -0.3112605967724278 2216 11 0.048974385189154646 2216 12 -0.03263065222291317 2216 13 -0.08554226909318885 2216 14 -0.26764023889482047 2216 15 -0.15912256326301133 2216 16 -0.032985490789663174 2216 17 -0.1280343165971658 2216 18 -0.19943577694959913 2216 19 -0.01490732518001181 2216 20 -0.08537179568758546 2216 21 -0.05778210245584603 2216 22 -0.037994594526900016 2216 23 -0.1450774265536623 2216 24 -0.26668837646047905 2216 25 -0.3551247379308222 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10802.0 3 12.0 4 155.0 5 1171.0 6 1597.0 7 1242.0 8 960.0 9 830.0 10 682.0 11 611.0 12 600.0 13 586.0 14 528.0 15 569.0 16 597.0 17 608.0 18 668.0 19 823.0 20 1050.0 21 1470.0 22 1744.0 23 2160.0 24 2533.0 25 3123.0 26 3596.0 27 3880.0 28 4556.0 29 5615.0 30 7178.0 31 9443.0 32 12273.0 33 16051.0 34 22333.0 35 30797.0 36 45391.0 37 78334.0 38 186956.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.00280276111017 19.68397349959 10.566756476504098 27.746467262795733 2 13.712271568786944 21.23724309961499 41.09907271839922 23.95141261319885 3 18.754293615001703 24.64887168673785 29.20806777031083 27.38876692794962 4 11.315554554042693 17.717171804716546 38.91238592142554 32.05488771981522 5 12.03870830742162 39.134740185870946 35.9137427058979 12.912808800809527 6 30.61780896970962 38.877873150877925 18.219668310330732 12.284649569081722 7 27.713088837311105 32.20707395777123 21.487563903354506 18.592273301563154 8 24.327967183345507 39.16393808521842 19.820444533049606 16.68765019838647 9 25.548784980302248 16.441229625070967 20.250761689715997 37.75922370491079 10 13.311419569843078 31.36015566428172 34.882753420323894 20.445671345551304 11 33.79747301727631 24.13569693542131 23.49127517069376 18.575554876608617 12 21.71852757067362 26.774180268372323 32.17434450720766 19.332947653746402 13 28.100346463107854 22.69755872430507 25.9929667339081 23.209128078678976 14 19.648598989435 23.530953969891055 28.626680302649483 28.193766738024458 15 22.291367826203775 31.65310960204201 23.80272412493012 22.252798446824094 16 20.675504092979317 30.71684556749191 28.029649724173495 20.57800061535528 17 20.40375019229811 30.09145806384514 28.168476977312075 21.336314766544678 18 19.91762021663153 29.874263576305303 29.822911633490136 20.385204573573034 19 21.456568795054633 28.683944245465558 29.082624267912976 20.776862691566834 20 21.52383098493893 28.711369580149114 29.68142294716817 20.083376487743788 21 21.704156373250118 28.218697178520348 28.54006847830798 21.537077969921555 22 20.962118897649738 29.416952134563175 28.77126383733855 20.849665130448535 23 20.865437117029668 28.76708470198733 28.746934070600876 21.620544110382127 24 20.653186630323148 28.70047365022686 29.962818673866032 20.68352104558396 25 21.17577719561689 28.528772543385795 29.12118986173318 21.174260399264135 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 191.0 1 191.0 2 223.0 3 255.0 4 255.0 5 255.0 6 392.0 7 529.0 8 529.0 9 529.0 10 844.5 11 1160.0 12 1160.0 13 1160.0 14 1959.0 15 2758.0 16 2758.0 17 2758.0 18 4618.5 19 6479.0 20 6479.0 21 6479.0 22 9997.0 23 13515.0 24 13515.0 25 13515.0 26 18631.0 27 23747.0 28 23747.0 29 23747.0 30 30426.0 31 37105.0 32 37105.0 33 37105.0 34 42693.0 35 48281.0 36 48281.0 37 48281.0 38 52605.0 39 56929.0 40 56929.0 41 56929.0 42 59569.5 43 62210.0 44 62210.0 45 62210.0 46 63276.0 47 64342.0 48 64342.0 49 64342.0 50 60728.5 51 57115.0 52 57115.0 53 57115.0 54 48249.0 55 39383.0 56 39383.0 57 39383.0 58 32062.5 59 24742.0 60 24742.0 61 24742.0 62 18974.0 63 13206.0 64 13206.0 65 13206.0 66 9689.0 67 6172.0 68 6172.0 69 6172.0 70 4208.5 71 2245.0 72 2245.0 73 2245.0 74 1522.5 75 800.0 76 800.0 77 800.0 78 530.5 79 261.0 80 261.0 81 261.0 82 164.5 83 68.0 84 68.0 85 68.0 86 46.5 87 25.0 88 25.0 89 25.0 90 14.5 91 4.0 92 4.0 93 4.0 94 2.5 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1191704006725544 2 0.10812005442837208 3 0.0179838968287673 4 0.0 5 0.0045501425711338955 6 0.34147736629081044 7 0.018417243740303863 8 0.011050346244182318 9 0.010833672788414038 10 0.0039001222038290533 11 0.005416836394207019 12 2.1667345576828075E-4 13 6.500203673048422E-4 14 0.0 15 0.0039001222038290533 16 4.333469115365615E-4 17 2.1667345576828075E-4 18 6.500203673048422E-4 19 2.1667345576828075E-4 20 6.500203673048422E-4 21 0.013867101169169968 22 2.1667345576828075E-4 23 0.0 24 4.333469115365615E-4 25 0.005416836394207019 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 461524.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.28233549121929 #Duplication Level Percentage of deduplicated Percentage of total 1 94.86691414612002 84.69939655810585 2 4.161837877960336 7.431572113602377 3 0.47724516659725025 1.2782868922709563 4 0.1545498916077696 0.5519430109062585 5 0.08268939812638244 0.3691351292543339 6 0.05264912055548248 0.2820381866851342 7 0.03291517119169806 0.20571203499616716 8 0.02766599299887095 0.19760675748983367 9 0.0206395084875373 0.1658469169042356 >10 0.10370485347609845 1.8274712839405027 >50 0.009958783426690855 0.5961598044869055 >100 0.00777270690683204 1.3690636664493145 >500 0.0012144854541924588 0.734952758687886 >1k 2.4289709083849176E-4 0.2908148862202582 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1341 0.2905591041852644 No Hit GGTATCAACGCAGAGTACTTTTTTT 896 0.19413941636837953 No Hit TATCAACGCAGAGTACTTTTTTTTT 700 0.15167141903779652 No Hit CACACACACACACACACACACACAC 681 0.1475546233781992 No Hit GTACATGGGAAGCAGTGGTATCAAC 557 0.12068711486293236 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 555 0.12025376795139582 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 476 0.10313656494570164 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 30 7.779259E-4 18.975296 18 GACGTGG 105 0.0 16.2663 7 ACGTGGA 100 1.8189894E-12 16.130749 8 CGTGGAA 105 5.456968E-12 15.364283 9 TCCAATT 40 0.0053173606 14.231471 12 GTAGGAC 275 0.0 14.197524 3 CCTGTAC 110 1.7826096E-10 13.851243 3 ACTGATC 55 1.9753381E-4 13.80171 8 GTCCTAC 280 0.0 13.62316 1 TAGGACC 135 1.8189894E-12 13.400903 4 GTGTTAA 50 0.0014544373 13.350697 1 GGCCAGA 50 0.0014544373 13.350697 1 AGTGCCA 50 0.0015115092 13.282706 10 TAGGACG 205 0.0 13.005241 4 GGACGTG 185 0.0 12.865756 6 ACTGTTC 105 2.0128937E-8 12.651567 8 AGGACGT 190 0.0 12.528544 5 CCCTGTA 115 5.007678E-9 12.435828 2 TGTAGGA 330 0.0 12.423267 2 TCCTACA 385 0.0 12.134355 2 >>END_MODULE