FastQCFastQC Report
Thu 26 May 2016
SRR1926939_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926939_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579512
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT9310.1606524109940778No Hit
GTACATGGGAAGCAGTGGTATCAAC6610.11406148621598863No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG655.5315344E-513.13072419
GAACTGT751.4492434E-512.6882016
TAGGACC1457.2759576E-1212.4748414
GTATAGC1251.3060344E-912.2145231
GAGTTAC1251.4406396E-912.13862413
TCTAAAC550.002984703812.1335333
GTCCAGG959.814921E-712.0538061
TCTATAC802.7578983E-511.9168623
GTCTTAG1052.5554618E-711.8146431
TTAAGCT657.868037E-411.717264
ATAGCCC855.1222152E-511.215873
CCAGTAC600.005716338311.1224043
TAGGACT1201.2320379E-711.1069864
TGTCCAC1201.300159E-711.0638510
GTAGGAC3800.011.0387773
AATGAGT1303.3325705E-810.94226910
TGGCGAG2350.010.89570718
CCTTATA700.001445858410.8954162
AGGAGTA700.001467992910.8756017
CTGTAGG3900.010.7660061
Produced by FastQC (version 0.11.5)