FastQCFastQC Report
Thu 26 May 2016
SRR1926939_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926939_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579512
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15070.2600463838540013No Hit
GGTATCAACGCAGAGTACTTTTTTT10330.17825342702135588No Hit
GTACATGGGAAGCAGTGGTATCAAC8040.13873742045030993No Hit
TATCAACGCAGAGTACTTTTTTTTT7990.13787462554701196No Hit
CCCATGTACTCTGCGTTGATACCAC6270.10819448087356258No Hit
GTCCTACAGTGGACATTTCTAAATT6190.10681400902828586No Hit
CTGTAGGACGTGGAATATGGCAAGA6190.10681400902828586No Hit
CACACACACACACACACACACACAC5870.10129212164717902No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAG602.476818E-514.3016251
GTCCTAT759.2570735E-713.9838121
GGACGTG2950.013.5497566
GGCGAGG1650.013.22732919
AGGACGT3150.012.9927085
TAGGACG3200.012.7896984
TTAGGAC2050.012.5379593
GACTGAT853.9778733E-612.2811647
TGGCGAG2800.012.20036418
TCTAAGC550.003017583912.1158073
CTGTGCG550.003090740212.0771279
AGACTGA1209.6952135E-911.8963636
ACTGATT658.081252E-411.6799898
CATCTAA907.1442264E-611.6531761
GACGTGA1800.011.5988787
CGTGAAA2050.011.572169
GTATAAG752.001145E-411.4413011
TGGACTG1251.7713319E-811.4214975
ATGGCGA3250.011.38700717
GACGTGG1604.5474735E-1111.2693647
Produced by FastQC (version 0.11.5)