FastQCFastQC Report
Thu 26 May 2016
SRR1926938_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926938_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575748
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11390.19782960600818414No Hit
GTCCTACAGTGGACATTTCTAAATT8660.15041302792193806No Hit
GTCCTACAGTGTGCATTTCTCATTT7750.13460750189318937No Hit
CTGTAGGACGTGGAATATGGCAAGA7130.12383890174173422No Hit
GGTATCAACGCAGAGTACTTTTTTT7030.12210203074956405No Hit
TATCAACGCAGAGTACTTTTTTTTT6210.10785968861376852No Hit
GTCCTAAAGTGTGTATTTCTCATTT5850.10160695304195586No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG5760.1000437691490027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAC502.0867992E-719.0684723
AAGGCGT456.667772E-414.8025926
GTGTTAT653.2077369E-614.6821591
TCCAACG400.005340290714.22309618
AGGCGTG759.461419E-713.956737
AAAGGCG551.9291101E-413.8413865
GACGTGG1850.012.8593957
TAGGACC1800.012.6967914
GGACGTG2900.012.4691796
GACAGTT550.003026124612.1112137
GTAGGAC4100.012.0922023
GGACCTG2450.012.0405916
AGAACTG802.8103108E-511.894945
CGTGGAA2000.011.8566999
AGGACGT2900.011.8129065
ACGTGGA2100.011.7734448
TGTAGGA4950.011.749262
GTGTTAA855.0680985E-511.2275341
CCCTACA855.1179944E-511.2167472
CTATACC855.1821335E-511.203054
Produced by FastQC (version 0.11.5)