FastQCFastQC Report
Thu 26 May 2016
SRR1926938_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926938_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575748
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18470.3208000722538333No Hit
GGTATCAACGCAGAGTACTTTTTTT12000.20842451906042228No Hit
TATCAACGCAGAGTACTTTTTTTTT9720.16882386043894204No Hit
GTCCTACAGTGGACATTTCTAAATT9210.15996581837887408No Hit
CTGTAGGACGTGGAATATGGCAAGA8150.14155498586187013No Hit
GTCCTACAGTGTGCATTTCTCATTT7640.13269694380180216No Hit
CACACACACACACACACACACACAC7250.12592314693233847No Hit
GTACATGGGAAGCAGTGGTATCAAC6610.11480717258244925No Hit
CTGTAGGACCTGGAATATGGCGAGA6540.11359136288793013No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6260.10872812410985362No Hit
GTCCTAAAGTGTGTATTTCTCATTT6220.10803337571298555No Hit
CTTTAGGACGTGAAATATGGCGAGG5870.10195432724038989No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA250.00607360618.97498319
TCCAACG453.5727833E-516.86665318
GCACCAT653.2766438E-614.652146
AGGACCT3350.014.4990585
GCTCTAG400.005147685814.3020481
GGACCTG2850.014.0352076
TAGGACC2550.013.8189774
CGGTTTC500.001513479213.28133513
GTAGGAC5000.013.1464083
CCTCTAG801.9131785E-613.110211
TAAGACA801.9377112E-613.095354
GTTCTAA751.4204692E-512.7129321
CTGTAGG5200.012.4684511
GTCTTAC701.0521556E-412.2588971
TGTACTG1251.3460522E-912.190585
TGTAGGA5650.011.97851852
TCTTACA1209.46784E-911.9152522
TCTACAC907.2113926E-611.6433563
GGCGAGG1157.1679096E-811.54999119
GTCCTAC4950.011.364591
Produced by FastQC (version 0.11.5)