##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926938_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 575748 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.8583408018786 31.0 30.0 33.0 26.0 34.0 2 30.16733188825667 31.0 30.0 34.0 26.0 34.0 3 30.21192084036766 31.0 30.0 34.0 26.0 34.0 4 33.78557285479064 35.0 35.0 37.0 28.0 37.0 5 33.797517316603795 37.0 35.0 37.0 30.0 37.0 6 33.72546148662262 37.0 35.0 37.0 29.0 37.0 7 33.72311844765418 37.0 35.0 37.0 29.0 37.0 8 33.74090053287202 37.0 35.0 37.0 29.0 37.0 9 35.27663317979394 39.0 35.0 39.0 29.0 39.0 10 35.16689593363763 39.0 35.0 39.0 29.0 39.0 11 35.17517038704433 39.0 35.0 39.0 29.0 39.0 12 35.08813578162668 39.0 35.0 39.0 29.0 39.0 13 35.09394561509549 39.0 35.0 39.0 29.0 39.0 14 36.199424053578994 40.0 36.0 41.0 29.0 41.0 15 36.171436114411165 40.0 36.0 41.0 29.0 41.0 16 36.15319896899338 40.0 36.0 41.0 29.0 41.0 17 36.141746041671006 40.0 36.0 41.0 29.0 41.0 18 36.11350104559634 40.0 36.0 41.0 28.0 41.0 19 36.2019529377436 40.0 37.0 41.0 29.0 41.0 20 36.15855374226224 40.0 37.0 41.0 28.0 41.0 21 36.110470205715 40.0 37.0 41.0 28.0 41.0 22 36.019411270208494 40.0 36.0 41.0 28.0 41.0 23 35.95353696408846 40.0 36.0 41.0 28.0 41.0 24 35.882745576189585 40.0 36.0 41.0 27.0 41.0 25 35.76756497634382 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.17185800741244606 1101 2 0.03340833970958457 1101 3 -0.012121681210938817 1101 4 -0.3205563499372843 1101 5 -0.19934793872172918 1101 6 -0.09015096314979587 1101 7 -0.13116595313316992 1101 8 -0.05538143294376141 1101 9 0.14351414533795293 1101 10 0.1569744347558597 1101 11 0.06626595711598782 1101 12 0.2603279751230545 1101 13 0.2913811159273223 1101 14 0.08291035080466003 1101 15 0.16811988647415887 1101 16 0.009538180788382533 1101 17 0.05223595381999502 1101 18 0.2020097310654947 1101 19 0.10246655337314792 1101 20 0.013031982968165323 1101 21 0.0664865365278473 1101 22 -0.09875921285020439 1101 23 -0.10028429506032666 1101 24 -0.1703233274873739 1101 25 0.050061878488151024 1102 1 -0.5328951025012678 1102 2 -0.21907337397902538 1102 3 -0.04427946595493282 1102 4 -0.2391169559978863 1102 5 -0.23393519055036194 1102 6 -0.20666089000454946 1102 7 -0.1483028392355763 1102 8 -0.16687568581732393 1102 9 -0.15929410962547053 1102 10 0.0359190533556486 1102 11 -0.007610740898641666 1102 12 0.09559443280330271 1102 13 0.00868780349262721 1102 14 -0.011512324221463643 1102 15 -0.09793288259584898 1102 16 -0.039051160904406856 1102 17 -0.023377943776658583 1102 18 -0.08381837760744304 1102 19 -0.012123310785682406 1102 20 0.008408158516758135 1102 21 -0.1034455428869947 1102 22 -0.09826287011248525 1102 23 0.009838484459002927 1102 24 -0.015869304498863812 1102 25 -0.09292401492877644 1103 1 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13 0.45484823391571894 2211 14 0.5753426525454657 2211 15 0.39449802960762526 2211 16 0.44638537614621754 2211 17 0.3934110021758954 2211 18 0.3999062494407326 2211 19 0.3207209054040945 2211 20 0.4142408804536615 2211 21 0.14206680732088017 2211 22 0.2910376923722282 2211 23 0.2849913335663672 2211 24 0.44749582144879696 2211 25 0.18345162703747775 2212 1 -0.02264768383771809 2212 2 -0.01530121344912061 2212 3 0.116114066092603 2212 4 0.15714010614900076 2212 5 0.007412461894602984 2212 6 -0.12455003865056824 2212 7 -0.12373143849526258 2212 8 -0.14097465328274694 2212 9 -0.1479727575896277 2212 10 -0.3181989088496877 2212 11 -0.3272240274756939 2212 12 -0.1788284193299461 2212 13 -0.3854871429324618 2212 14 -0.3463847185327822 2212 15 -0.43528920443493035 2212 16 -0.4968755326168548 2212 17 -0.4765583451014166 2212 18 -0.30997023811787017 2212 19 -0.3977067902323981 2212 20 -0.2789749507760533 2212 21 -0.4269649272626026 2212 22 -0.3459664547608412 2212 23 -0.3845678081573993 2212 24 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2214 11 0.49979531217238105 2214 12 0.35343990031345385 2214 13 0.47954871450053815 2214 14 0.5103023781834253 2214 15 0.3269059175162923 2214 16 0.6247218306485962 2214 17 0.32481259510024785 2214 18 0.23172750798634212 2214 19 0.5363658592634408 2214 20 0.37203853844874146 2214 21 0.582284762691927 2214 22 0.63674401668802 2214 23 0.6383339928023943 2214 24 0.3615298008265242 2214 25 0.5228144572985229 2215 1 0.29132285181271556 2215 2 0.25817819011234633 2215 3 0.06725116113658558 2215 4 0.3851446473776363 2215 5 0.37324508007382207 2215 6 0.37068524705350114 2215 7 0.21332963566621288 2215 8 0.26993502275243486 2215 9 0.3661023693502301 2215 10 0.21073354484408213 2215 11 0.17223066751108007 2215 12 0.23813771812267248 2215 13 0.23591026820963634 2215 14 0.20696577849932396 2215 15 0.02884581666211261 2215 16 0.145882638018918 2215 17 0.4234720489484971 2215 18 0.3764391518556707 2215 19 0.30659486177361117 2215 20 0.349059984502496 2215 21 0.3242061376018768 2215 22 0.2956117115840087 2215 23 0.34392840152578685 2215 24 0.3696028328501839 2215 25 0.39025654668915166 2216 1 -0.3678878450378491 2216 2 -0.32241180715151785 2216 3 -1.9811748407931091 2216 4 -1.5554992085576131 2216 5 -0.5832035597787169 2216 6 -0.27710928355521247 2216 7 -0.6572079429976867 2216 8 -0.39360668891646355 2216 9 -0.678493042582204 2216 10 -0.6435168259743094 2216 11 -0.6558356045760192 2216 12 -0.5162725006241757 2216 13 -0.6706146954285543 2216 14 -0.779108730796338 2216 15 -1.0317055866874867 2216 16 -0.5761583752153427 2216 17 -0.9008680383198282 2216 18 -0.812401014616583 2216 19 -0.886918336481024 2216 20 -0.8331594535816294 2216 21 -0.5373633962470024 2216 22 -0.839438186890952 2216 23 -0.7511595148348675 2216 24 -0.692806262640957 2216 25 -0.7726227321230823 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14032.0 3 13.0 4 193.0 5 1498.0 6 2025.0 7 1612.0 8 1228.0 9 1044.0 10 918.0 11 868.0 12 782.0 13 724.0 14 774.0 15 733.0 16 739.0 17 791.0 18 903.0 19 1096.0 20 1340.0 21 1719.0 22 2292.0 23 2681.0 24 3342.0 25 3864.0 26 4496.0 27 5190.0 28 5874.0 29 7218.0 30 9179.0 31 11892.0 32 15257.0 33 20318.0 34 27539.0 35 38536.0 36 56510.0 37 99175.0 38 229353.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.117316861951316 19.395608790765454 10.57232487853399 27.91474946874924 2 13.663394226455644 21.260254631320137 41.16736740705192 23.9089837351723 3 18.7685984438542 25.00464689665475 28.828304551526703 27.398450107964344 4 11.256313526056537 17.847565254243175 38.35532212009421 32.540799099606076 5 12.089851417216426 39.37756424286007 35.5823979600699 12.950186379853607 6 30.71683300855124 38.78787139326289 18.188901167472523 12.306394430713347 7 28.02120865719041 32.33243110834692 21.271025374643422 18.375334859819255 8 24.538199908977525 38.99114441653552 19.629239753891905 16.84141592059505 9 26.17387875492681 16.075855036765407 19.888062229340413 37.86220397896737 10 13.520475515535546 31.3521344005336 34.51482685787338 20.612563226057475 11 33.98979354332185 23.662096110221395 23.841699177021923 18.506411169434823 12 22.41542218563176 26.346696958012444 32.095452476977535 19.142428379378263 13 28.422676168624434 22.810430384495806 25.818727518102758 22.948165928777005 14 19.763160271507672 23.669556819997638 28.311518233671674 28.255764674823013 15 22.32064696319146 32.00355718593244 23.592584101046313 22.083211749829783 16 20.803828083613404 30.042640405040427 28.575497833242146 20.57803367810402 17 20.342789454395767 30.00797225170083 28.239487135842655 21.409751158060743 18 19.890367370050075 29.354171406934714 30.357921356999068 20.397539866016142 19 21.861376370830193 28.248915320297492 28.689561021700545 21.200147287171774 20 22.014353751185432 28.55135495659484 29.36769850174906 20.066592790470665 21 22.02838685470801 27.739282841951123 28.266650917422425 21.96567938591844 22 21.4300353626934 29.299971515315725 28.54130626593579 20.728686856055077 23 21.094892539418424 28.732461884233672 28.632938830664912 21.53970674568299 24 21.1191721363724 28.21742883592967 29.798505246227947 20.864893781469988 25 21.324901081417202 28.520858880787042 29.06608351889614 21.088156518899616 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 276.0 1 276.0 2 292.0 3 308.0 4 308.0 5 308.0 6 463.5 7 619.0 8 619.0 9 619.0 10 990.0 11 1361.0 12 1361.0 13 1361.0 14 2136.5 15 2912.0 16 2912.0 17 2912.0 18 4986.0 19 7060.0 20 7060.0 21 7060.0 22 11276.5 23 15493.0 24 15493.0 25 15493.0 26 22121.0 27 28749.0 28 28749.0 29 28749.0 30 37472.0 31 46195.0 32 46195.0 33 46195.0 34 53475.5 35 60756.0 36 60756.0 37 60756.0 38 65650.5 39 70545.0 40 70545.0 41 70545.0 42 74060.0 43 77575.0 44 77575.0 45 77575.0 46 79159.0 47 80743.0 48 80743.0 49 80743.0 50 76099.5 51 71456.0 52 71456.0 53 71456.0 54 60645.0 55 49834.0 56 49834.0 57 49834.0 58 40766.0 59 31698.0 60 31698.0 61 31698.0 62 24492.5 63 17287.0 64 17287.0 65 17287.0 66 12653.0 67 8019.0 68 8019.0 69 8019.0 70 5611.0 71 3203.0 72 3203.0 73 3203.0 74 2180.5 75 1158.0 76 1158.0 77 1158.0 78 761.5 79 365.0 80 365.0 81 365.0 82 228.5 83 92.0 84 92.0 85 92.0 86 63.5 87 35.0 88 35.0 89 35.0 90 21.5 91 8.0 92 8.0 93 8.0 94 4.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1198440984597428 2 0.11150711769732592 3 0.016500274425616763 4 0.0 5 0.007294858167114779 6 0.35258481141054765 7 0.023274071295080485 8 0.011463348548323225 9 0.012331784044408317 10 0.006252735571812669 11 0.005905361373378631 12 0.0010421225953021114 13 0.00121580969451913 14 0.0 15 0.0020842451906042227 16 5.210612976510557E-4 17 1.7368709921701856E-4 18 0.00121580969451913 19 3.473741984340371E-4 20 0.0017368709921701856 21 0.010247538853804094 22 0.0 23 3.473741984340371E-4 24 1.7368709921701856E-4 25 0.003821116182774408 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 575748.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.17861125296155 #Duplication Level Percentage of deduplicated Percentage of total 1 94.57839061008862 82.45212747927665 2 4.31240282711064 7.518985792617016 3 0.5165248169740259 1.3508974866445713 4 0.17773660965996033 0.6197932319586019 5 0.09336402933572135 0.40696732092344745 6 0.060649181866628266 0.31723868692565704 7 0.044439257461107017 0.27119069254004835 8 0.030980097240900027 0.2160641483154696 9 0.025205808505590323 0.1977666642922906 >10 0.1333738212003057 2.2805215892740454 >50 0.013965256105374484 0.8609255656507381 >100 0.010374147560947464 1.5997511429698879 >500 0.002194531214730297 1.3778243698971657 >1k 3.990056754055085E-4 0.5299458287145499 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1847 0.3208000722538333 No Hit GGTATCAACGCAGAGTACTTTTTTT 1200 0.20842451906042228 No Hit TATCAACGCAGAGTACTTTTTTTTT 972 0.16882386043894204 No Hit GTCCTACAGTGGACATTTCTAAATT 921 0.15996581837887408 No Hit CTGTAGGACGTGGAATATGGCAAGA 815 0.14155498586187013 No Hit GTCCTACAGTGTGCATTTCTCATTT 764 0.13269694380180216 No Hit CACACACACACACACACACACACAC 725 0.12592314693233847 No Hit GTACATGGGAAGCAGTGGTATCAAC 661 0.11480717258244925 No Hit CTGTAGGACCTGGAATATGGCGAGA 654 0.11359136288793013 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 626 0.10872812410985362 No Hit GTCCTAAAGTGTGTATTTCTCATTT 622 0.10803337571298555 No Hit CTTTAGGACGTGAAATATGGCGAGG 587 0.10195432724038989 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.7368709921701856E-4 0.0 4 0.0 0.0 0.0 1.7368709921701856E-4 0.0 5 0.0 0.0 0.0 1.7368709921701856E-4 0.0 6 0.0 0.0 0.0 1.7368709921701856E-4 0.0 7 0.0 0.0 0.0 1.7368709921701856E-4 0.0 8 0.0 0.0 0.0 1.7368709921701856E-4 0.0 9 0.0 0.0 0.0 1.7368709921701856E-4 0.0 10 0.0 0.0 0.0 1.7368709921701856E-4 0.0 11 0.0 0.0 0.0 1.7368709921701856E-4 0.0 12 0.0 0.0 0.0 1.7368709921701856E-4 0.0 13 0.0 0.0 0.0 1.7368709921701856E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 25 0.006073606 18.974983 19 TCCAACG 45 3.5727833E-5 16.866653 18 GCACCAT 65 3.2766438E-6 14.65214 6 AGGACCT 335 0.0 14.499058 5 GCTCTAG 40 0.0051476858 14.302048 1 GGACCTG 285 0.0 14.035207 6 TAGGACC 255 0.0 13.818977 4 CGGTTTC 50 0.0015134792 13.281335 13 GTAGGAC 500 0.0 13.146408 3 CCTCTAG 80 1.9131785E-6 13.11021 1 TAAGACA 80 1.9377112E-6 13.09535 4 GTTCTAA 75 1.4204692E-5 12.712932 1 CTGTAGG 520 0.0 12.468451 1 GTCTTAC 70 1.0521556E-4 12.258897 1 TGTACTG 125 1.3460522E-9 12.19058 5 TGTAGGA 565 0.0 11.9785185 2 TCTTACA 120 9.46784E-9 11.915252 2 TCTACAC 90 7.2113926E-6 11.643356 3 GGCGAGG 115 7.1679096E-8 11.549991 19 GTCCTAC 495 0.0 11.36459 1 >>END_MODULE