FastQCFastQC Report
Thu 26 May 2016
SRR1926937_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926937_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666723
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT19040.2855758688390831No Hit
GTCCTACAGTGTGCATTTCTCATTT17890.26832732634092415No Hit
CTGTAGGACGTGGAATATGGCAAGA15980.23967974706137332No Hit
GTCCTAAAGTGTGTATTTCTCATTT12650.18973396747974797No Hit
CTGTAGGACCTGGAATATGGCGAGA11810.1771350320897884No Hit
CTTTAGGACGTGAAATATGGCGAGG9630.14443779500632198No Hit
GTATCAACGCAGAGTACTTTTTTTT7330.10994071001000415No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTCGA350.002192042316.25935417
TAAGTAG400.005208408514.277275
ATACTGC500.001477382713.3244486
GCCGGTT500.001516378413.27847211
CCGGTTT500.001516378413.27847212
TAGGACC3700.013.1216284
TGTAGGA9100.012.7731372
TCTGAAC751.433459E-512.7023813
GACGTGG4200.012.6889957
GGACCTG6050.012.2704496
TTGGACC701.0683197E-412.2395034
CTGTAGG9150.012.1909761
GAACAGT1251.3624231E-912.1823526
GTAGGAC9350.012.1253
TAGGCAG550.003021688212.1140475
TGCCGGT550.003102700212.07133810
GTCCTAC11400.012.0429081
AGGACGT7400.011.8334135
ATAGGAC1700.011.7683833
GGACGTG7150.011.7138016
Produced by FastQC (version 0.11.5)