##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926937_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 666723 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.259380582340793 31.0 28.0 31.0 16.0 34.0 2 28.30261142933422 31.0 28.0 31.0 16.0 34.0 3 27.955765737795158 31.0 28.0 33.0 16.0 34.0 4 31.90542099192618 35.0 32.0 37.0 19.0 37.0 5 31.915995098414182 35.0 32.0 37.0 19.0 37.0 6 31.391183444998898 35.0 32.0 37.0 17.0 37.0 7 31.307779992590625 35.0 32.0 37.0 17.0 37.0 8 31.03075790095737 35.0 32.0 37.0 11.0 37.0 9 33.15009831669224 37.0 33.0 39.0 17.0 39.0 10 33.285455878978226 37.0 33.0 39.0 17.0 39.0 11 33.44417096755324 38.0 34.0 39.0 18.0 39.0 12 33.29411314743904 38.0 34.0 39.0 17.0 39.0 13 33.40590470105276 38.0 34.0 39.0 17.0 39.0 14 34.42952020554263 39.0 34.0 41.0 17.0 41.0 15 34.02607079701765 39.0 33.0 41.0 11.0 41.0 16 34.102052876531936 39.0 34.0 41.0 17.0 41.0 17 34.32585796500196 39.0 34.0 41.0 17.0 41.0 18 34.3703862023659 39.0 34.0 41.0 17.0 41.0 19 33.74718136317481 39.0 33.0 41.0 10.0 41.0 20 34.03714436130147 39.0 34.0 41.0 10.0 41.0 21 34.01996931259308 39.0 34.0 41.0 10.0 41.0 22 34.04578813090294 39.0 34.0 41.0 10.0 41.0 23 34.04584962570663 39.0 34.0 41.0 2.0 41.0 24 32.748184778386225 39.0 32.0 41.0 2.0 41.0 25 33.33302735918815 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.08760169442649612 1101 2 -0.25533452074765606 1101 3 -1.038773400863871 1101 4 -0.34555592869199714 1101 5 -0.2659029103624633 1101 6 -1.9298473184556784 1101 7 -0.4194653797625456 1101 8 0.19429453893521398 1101 9 -0.05770549644851286 1101 10 -9.097748565714948 1101 11 -3.767161263263759 1101 12 -1.4865548815088836 1101 13 -0.7571207886243059 1101 14 -0.7011857317203933 1101 15 -1.9489342454355665 1101 16 -0.47792706418809416 1101 17 -0.24664091247873898 1101 18 -0.018294830379566918 1101 19 -0.7680653098718793 1101 20 -0.06677368152488583 1101 21 -0.23844014899844268 1101 22 -0.047114945189335344 1101 23 -0.09530963419062033 1101 24 -0.14546044657242874 1101 25 -0.10817533291214687 1102 1 -0.5416449227413338 1102 2 -0.4449309324253754 1102 3 -0.8846645563937443 1102 4 -0.6726191650374709 1102 5 -0.4590073379839268 1102 6 -1.790621589088044 1102 7 -0.7146078255802486 1102 8 -0.3078923397924207 1102 9 -0.12159506543851961 1102 10 -0.27016235881839634 1102 11 -0.44921671381170114 1102 12 -0.278778877434803 1102 13 -0.44208783364481974 1102 14 -0.6339213377884221 1102 15 -0.22051559477793603 1102 16 -0.4702891478438005 1102 17 -0.46362962062395496 1102 18 -0.737873907640143 1102 19 -0.42537857314935223 1102 20 -0.28271742791354626 1102 21 -0.5286672592433774 1102 22 -0.5750272741711058 1102 23 -0.8533730761986718 1102 24 -4.5876908082447585 1102 25 -2.64426519766452 1103 1 -0.013307197156855466 1103 2 -0.21512372913349864 1103 3 0.09865452138762976 1103 4 0.059099052787363604 1103 5 -0.17369017121708552 1103 6 0.6819056505747554 1103 7 0.4979039256179867 1103 8 0.5243332986251374 1103 9 0.3430954734984013 1103 10 0.45755081947617526 1103 11 0.10003429178264156 1103 12 -0.06336207591832022 1103 13 0.014983690697498275 1103 14 0.25850896412104873 1103 15 0.5011717231748136 1103 16 0.3120574701228165 1103 17 0.1445025938411888 1103 18 -0.14606815302057186 1103 19 -1.7394938813004472 1103 20 -0.5459866497915797 1103 21 -0.0827054332998145 1103 22 -0.07119368654954883 1103 23 -0.03411228436055325 1103 24 1.4905240113647196 1103 25 0.646476784550714 1104 1 -0.6237647283694052 1104 2 -0.4248128931074646 1104 3 -1.8061396199069897 1104 4 -0.8464061884936029 1104 5 -0.7937522929118934 1104 6 -2.052311722261603 1104 7 -0.7181832419664715 1104 8 -0.30903227124090904 1104 9 -0.28820013171405634 1104 10 -0.23787273063835812 1104 11 -0.5534153964711308 1104 12 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-2.548858396335838 2216 12 -2.2279020546325725 2216 13 -2.0997668810739754 2216 14 -2.280436598705876 2216 15 -3.3628117690468287 2216 16 -2.483358734220378 2216 17 -2.662362249195276 2216 18 -2.4419929131784777 2216 19 -1.5759106291096003 2216 20 -2.4014190448913304 2216 21 -5.1161182107882475 2216 22 -3.614684172121283 2216 23 -3.6427139540087374 2216 24 -3.910622739539175 2216 25 -3.324590258013238 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38730.0 3 2.0 4 357.0 5 1883.0 6 2466.0 7 1975.0 8 1529.0 9 1374.0 10 1287.0 11 1282.0 12 1223.0 13 1198.0 14 1135.0 15 1318.0 16 1376.0 17 1448.0 18 1708.0 19 2107.0 20 2697.0 21 3324.0 22 4191.0 23 5214.0 24 6051.0 25 6808.0 26 7818.0 27 8692.0 28 9536.0 29 11115.0 30 13424.0 31 16340.0 32 21028.0 33 27780.0 34 36965.0 35 51367.0 36 74704.0 37 119581.0 38 177690.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.07942978421505 18.78889269674068 12.359835981794461 25.771841537249806 2 13.561465266597786 20.058059206574235 43.73663406780883 22.64384145901915 3 19.36320520945656 24.017294771019994 28.95417396292948 27.66532605659397 4 11.101838774410552 16.540414463444396 39.822351367861444 32.535395394283604 5 11.995355242904592 38.28594060831916 37.39839593853798 12.320308210238268 6 31.551833032541072 37.98518691876209 18.555507048740346 11.907472999956493 7 27.642419484375143 31.459072164018153 22.385704961031852 18.512803390574852 8 23.63254110046379 39.27447831134692 20.67369871089215 16.419281877297145 9 25.832632003732424 15.725684865232203 20.387613994188342 38.054069136847026 10 13.58158252482542 30.128110867366694 35.69548686030844 20.59481974749945 11 34.34157054585222 22.688412547685374 24.965759538200054 18.004257368262355 12 23.064621865253727 25.055767993147327 32.75542640950134 19.12418373209761 13 29.060470139961897 22.578119140126912 25.833395838646283 22.52801488126491 14 19.233994548463023 23.52066678567839 28.169324678480827 29.076013987377756 15 22.03007428564571 33.28527968125577 23.003648277269072 21.68099775582944 16 20.486867450359142 29.166141604709356 29.9228308940455 20.424160050886 17 20.37150105086612 29.81729481107662 29.043659324355342 20.767544813701914 18 20.101857893986786 28.57901487365271 31.554195450147386 19.76493178221312 19 23.05605813747641 27.430021093438228 27.77102667734841 21.742894091736954 20 22.488865153217592 28.49575683204796 29.292785339355927 19.722592675378525 21 21.739619601660323 27.696113360445352 27.598006051450835 22.96626098644349 22 21.664083912265358 29.949400015901567 28.021560123672177 20.364955948160894 23 20.835464799677165 29.344028418884516 28.64975795043197 21.170748831006346 24 22.529079797303986 27.64388386682548 29.53283309381441 20.29420324205612 25 21.45157685193658 28.63250491664579 29.06408965921757 20.851828572200063 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 147.0 1 147.0 2 204.0 3 261.0 4 261.0 5 261.0 6 432.0 7 603.0 8 603.0 9 603.0 10 922.5 11 1242.0 12 1242.0 13 1242.0 14 2230.5 15 3219.0 16 3219.0 17 3219.0 18 5651.5 19 8084.0 20 8084.0 21 8084.0 22 12928.0 23 17772.0 24 17772.0 25 17772.0 26 26152.5 27 34533.0 28 34533.0 29 34533.0 30 45840.5 31 57148.0 32 57148.0 33 57148.0 34 65451.5 35 73755.0 36 73755.0 37 73755.0 38 79075.0 39 84395.0 40 84395.0 41 84395.0 42 86766.0 43 89137.0 44 89137.0 45 89137.0 46 89610.0 47 90083.0 48 90083.0 49 90083.0 50 83589.5 51 77096.0 52 77096.0 53 77096.0 54 65839.0 55 54582.0 56 54582.0 57 54582.0 58 45336.0 59 36090.0 60 36090.0 61 36090.0 62 28300.0 63 20510.0 64 20510.0 65 20510.0 66 15647.5 67 10785.0 68 10785.0 69 10785.0 70 7701.5 71 4618.0 72 4618.0 73 4618.0 74 3203.5 75 1789.0 76 1789.0 77 1789.0 78 1205.0 79 621.0 80 621.0 81 621.0 82 402.0 83 183.0 84 183.0 85 183.0 86 117.0 87 51.0 88 51.0 89 51.0 90 33.0 91 15.0 92 15.0 93 15.0 94 8.5 95 2.0 96 2.0 97 2.0 98 2.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21463186360752518 2 0.1276392144863759 3 0.12853913844280157 4 0.0695941192969194 5 0.024747908801706256 6 0.02249809891064205 7 0.2731269207751945 8 0.26502760516736334 9 0.019798327041365003 10 0.040346590713084746 11 0.01709855517208796 12 0.018298453780655533 13 0.01694856784601701 14 0.018298453780655533 15 0.01604864388959133 16 0.020248289019577845 17 0.019498352389223112 18 0.016198631215662278 19 0.024747908801706256 20 0.018298453780655533 21 0.015898656563520384 22 0.018298453780655533 23 0.01859842843279743 24 0.016348618541733223 25 0.01649860586780417 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 666723.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.59245950712794 #Duplication Level Percentage of deduplicated Percentage of total 1 94.24473693220787 80.66638829629927 2 4.410412500760082 7.549961067620764 3 0.6251526857454166 1.6052506782131053 4 0.23274913887795134 0.796862849789198 5 0.11557481198906765 0.49461662076090984 6 0.07293654065017317 0.3745690741313963 7 0.045629302534258925 0.27338669606514326 8 0.03637529853204905 0.2490761013331279 9 0.027807555578496467 0.21421053673602014 >10 0.15498771417018833 2.5493820355356154 >50 0.01804535282954799 1.0609323622633735 >100 0.014015430226093901 2.5853205861357664 >500 7.007715106133078E-4 0.41986530002142897 >1k 8.759643882666349E-4 1.1601777950949272 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1904 0.2855758688390831 No Hit GTCCTACAGTGTGCATTTCTCATTT 1789 0.26832732634092415 No Hit CTGTAGGACGTGGAATATGGCAAGA 1598 0.23967974706137332 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1265 0.18973396747974797 No Hit CTGTAGGACCTGGAATATGGCGAGA 1181 0.1771350320897884 No Hit CTTTAGGACGTGAAATATGGCGAGG 963 0.14443779500632198 No Hit GTATCAACGCAGAGTACTTTTTTTT 733 0.10994071001000415 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 1.4998732607094702E-4 0.0 7 0.0 0.0 0.0 1.4998732607094702E-4 0.0 8 0.0 0.0 0.0 1.4998732607094702E-4 0.0 9 0.0 0.0 0.0 1.4998732607094702E-4 0.0 10 0.0 0.0 0.0 1.4998732607094702E-4 0.0 11 0.0 0.0 0.0 1.4998732607094702E-4 0.0 12 0.0 0.0 0.0 1.4998732607094702E-4 0.0 13 0.0 0.0 0.0 1.4998732607094702E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGTCGA 35 0.0021920423 16.259354 17 TAAGTAG 40 0.0052084085 14.27727 5 ATACTGC 50 0.0014773827 13.324448 6 GCCGGTT 50 0.0015163784 13.278472 11 CCGGTTT 50 0.0015163784 13.278472 12 TAGGACC 370 0.0 13.121628 4 TGTAGGA 910 0.0 12.773137 2 TCTGAAC 75 1.433459E-5 12.702381 3 GACGTGG 420 0.0 12.688995 7 GGACCTG 605 0.0 12.270449 6 TTGGACC 70 1.0683197E-4 12.239503 4 CTGTAGG 915 0.0 12.190976 1 GAACAGT 125 1.3624231E-9 12.182352 6 GTAGGAC 935 0.0 12.125 3 TAGGCAG 55 0.0030216882 12.114047 5 TGCCGGT 55 0.0031027002 12.071338 10 GTCCTAC 1140 0.0 12.042908 1 AGGACGT 740 0.0 11.833413 5 ATAGGAC 170 0.0 11.768383 3 GGACGTG 715 0.0 11.713801 6 >>END_MODULE