FastQCFastQC Report
Thu 26 May 2016
SRR1926937_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926937_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666723
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA19280.28917556466478583No Hit
GTCCTACAGTGGACATTTCTAAATT19170.28752570407800543No Hit
GTCCTACAGTGTGCATTTCTCATTT17720.26577754179771806No Hit
CTGTAGGACCTGGAATATGGCGAGA14480.21718164815073127No Hit
GTATCAACGCAGAGTACTTTTTTTT13250.1987332070440048No Hit
GTCCTAAAGTGTGTATTTCTCATTT13030.19543348587044396No Hit
CTTTAGGACGTGAAATATGGCGAGG12730.19093386608831553No Hit
GGTATCAACGCAGAGTACTTTTTTT8480.12718925250816307No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA307.771737E-418.97982419
GTAAGCC350.002138344316.3198263
TCCAACG704.4784974E-714.91272118
TAGGACC5200.014.8268424
TGCCGGT456.8097905E-414.76208810
AATCCCG653.4024488E-614.59986619
TGTCGAG400.005312226214.2348718
CGGTTTC551.9752292E-413.80351113
CTAGGAC1204.9112714E-1113.4865243
TGTAGGA11300.013.4053412
GCCGGTT500.001510021213.28587711
GTAGGAC10750.013.1064653
CTGTAGG11400.012.9554191
AGGACCT7350.012.8207935
GACGTGG3900.012.4108167
AATGTCC1302.0008883E-1012.4098868
ACTGTTC2000.012.3368868
GGACCTG7300.012.2566576
GTGTAAC701.0590512E-412.2509311
TCTAGGA1251.3424142E-912.1946452
Produced by FastQC (version 0.11.5)