FastQCFastQC Report
Thu 26 May 2016
SRR1926936_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926936_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737161
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT20290.27524516353957956No Hit
GTCCTACAGTGTGCATTTCTCATTT15850.21501408783155918No Hit
CTGTAGGACGTGGAATATGGCAAGA15630.21202966516134197No Hit
GTCCTAAAGTGTGTATTTCTCATTT15490.21013048709847643No Hit
CTTTAGGACGTGAAATATGGCGAGG11500.15600391230680952No Hit
CTGTAGGACCTGGAATATGGCGAGA10690.14501581065737335No Hit
GTATCAACGCAGAGTACTTTTTTTT10000.1356555759189648No Hit
GTACATGGGAAGCAGTGGTATCAAC8360.11340806146825455No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGACGT400.005204635714.2791164
TCCAACG400.00534257814.22293318
TAGGACC4050.013.1626414
TAGGACA2650.012.5728054
GTAGGAC9400.012.473243
GGCGAGG3300.012.35527519
TGTAGGA10050.012.3304612
GGTTATA701.0439372E-412.2701621
TAGAACC701.0688287E-412.2392424
GGACCTG5950.012.1575916
GTCTAGG550.002962743912.1462211
AGTCGTC550.003039368712.1048968
AATCCCG802.9177872E-511.85244419
CTGTAGG10000.011.8338891
GCTATAC1052.5949157E-711.8020123
GTCCTAC10800.011.7525931
GGAGTGT657.88752E-411.71460156
CTTATAC907.168548E-611.6507043
AGGACCT6050.011.6419915
TTTACAC752.00651E-411.4388733
Produced by FastQC (version 0.11.5)