##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926936_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 737161 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.437468884002275 31.0 28.0 31.0 22.0 34.0 2 28.446584938704028 31.0 28.0 31.0 22.0 34.0 3 28.12232334591765 31.0 28.0 31.0 16.0 34.0 4 32.017035627223905 35.0 32.0 37.0 20.0 37.0 5 32.051051262885586 35.0 33.0 37.0 20.0 37.0 6 31.580993839880296 35.0 32.0 37.0 17.0 37.0 7 31.482483473759462 35.0 32.0 37.0 17.0 37.0 8 31.176449920709317 35.0 32.0 37.0 16.0 37.0 9 33.3691920760865 37.0 33.0 39.0 17.0 39.0 10 33.52391268664512 38.0 34.0 39.0 18.0 39.0 11 33.677956375879894 38.0 34.0 39.0 22.0 39.0 12 33.56074453206288 38.0 34.0 39.0 19.0 39.0 13 33.66313871732227 38.0 34.0 39.0 22.0 39.0 14 34.69382672170666 39.0 34.0 41.0 21.0 41.0 15 34.282467466401506 39.0 34.0 41.0 17.0 41.0 16 34.36694019352625 39.0 34.0 41.0 17.0 41.0 17 34.592099961880784 39.0 34.0 41.0 19.0 41.0 18 34.64664978206932 39.0 35.0 41.0 19.0 41.0 19 34.01854683033964 39.0 34.0 41.0 11.0 41.0 20 34.3106024328471 39.0 34.0 41.0 18.0 41.0 21 34.29482161970044 39.0 34.0 41.0 17.0 41.0 22 34.31958011886142 39.0 34.0 41.0 16.0 41.0 23 34.30768855107636 39.0 34.0 41.0 10.0 41.0 24 33.035532265000455 39.0 32.0 41.0 2.0 41.0 25 33.61254597028329 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.1259608444279472 1101 2 0.048424026288422795 1101 3 -0.8626573122819003 1101 4 -0.018820638399493106 1101 5 0.06622598491809839 1101 6 -2.116776029116828 1101 7 -0.46483496525497614 1101 8 0.04484943767741356 1101 9 0.09121314936206204 1101 10 -8.914370719876597 1101 11 -3.553186692374311 1101 12 -0.9970856829656967 1101 13 -0.41499116401431735 1101 14 -0.20239677984362459 1101 15 -1.5688017803370258 1101 16 0.027328425242750143 1101 17 0.07850722274142896 1101 18 0.2247619386962043 1101 19 -0.14398257843534168 1101 20 0.41573484040727493 1101 21 0.3160705958137484 1101 22 0.4861001914373446 1101 23 0.5565155444686027 1101 24 0.2656520432031897 1101 25 0.5085985209619039 1102 1 0.08241433914411189 1102 2 4.792232465469226E-4 1102 3 -0.15333443190049323 1102 4 -0.16236882242292694 1102 5 0.014746595643565286 1102 6 -0.6889181847863526 1102 7 0.10600532785295869 1102 8 0.4897901362009627 1102 9 0.4768741950489641 1102 10 0.7482269617840132 1102 11 0.2899653091029819 1102 12 0.3096913888088082 1102 13 0.2723058134150307 1102 14 0.10726352719785126 1102 15 0.5828689852521691 1102 16 0.4360436018881231 1102 17 0.1561531261971183 1102 18 0.04851106785003623 1102 19 0.46751678784045936 1102 20 0.39073672898932443 1102 21 0.21084467943177287 1102 22 -0.013011755313037554 1102 23 -0.43402056102339515 1102 24 -3.4359671011432376 1102 25 -2.166974978454732 1103 1 -0.20229249800387805 1103 2 -0.17704163954421404 1103 3 -0.3151488666917217 1103 4 -0.12812637297628626 1103 5 -0.6095417002741037 1103 6 0.325327238757918 1103 7 -0.06169093108508861 1103 8 0.15525036100577694 1103 9 -0.05434164798589336 1103 10 -0.21042804037094953 1103 11 -0.3390958254060479 1103 12 -0.29133341466130247 1103 13 -0.21181258927610713 1103 14 -0.14407376655814375 1103 15 0.21149324666359348 1103 16 -0.05951751539802075 1103 17 -0.3955873510195289 1103 18 -0.4438507941367149 1103 19 -2.151441656286412 1103 20 -0.679972862139465 1103 21 -0.41058629839943706 1103 22 -0.5149621416516794 1103 23 -0.45001767766095213 1103 24 0.9472773033105142 1103 25 0.2056912160529336 1104 1 -0.5894523326146306 1104 2 -0.47572722498115994 1104 3 -1.6175033803150036 1104 4 -0.749848461040564 1104 5 -0.5227648407699732 1104 6 -2.205165779195916 1104 7 -0.6012108622895589 1104 8 -0.0810301140966736 1104 9 -0.10287932637013597 1104 10 -0.16130623328840699 1104 11 -0.1582134160959825 1104 12 -0.19832945991836226 1104 13 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-2.0791212835398767 2216 14 -2.300184870029703 2216 15 -2.9523490791814595 2216 16 -2.3192392219507667 2216 17 -2.5884087735338923 2216 18 -2.340664185958424 2216 19 -1.5834428052744016 2216 20 -2.5199747567473167 2216 21 -5.342924351445404 2216 22 -3.7047728183781032 2216 23 -3.6233147078631376 2216 24 -3.6448884932116563 2216 25 -3.1436492617855016 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38916.0 3 7.0 4 295.0 5 2057.0 6 2650.0 7 2134.0 8 1667.0 9 1371.0 10 1275.0 11 1307.0 12 1325.0 13 1239.0 14 1274.0 15 1343.0 16 1396.0 17 1500.0 18 1785.0 19 2207.0 20 2825.0 21 3677.0 22 4490.0 23 5538.0 24 6542.0 25 7554.0 26 8622.0 27 9342.0 28 10536.0 29 12379.0 30 14942.0 31 18323.0 32 23451.0 33 30837.0 34 41469.0 35 58328.0 36 85046.0 37 136848.0 38 192664.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.75896292469376 18.947153753076012 12.679564395741846 25.614318926488384 2 13.844578082485794 20.23755995712836 42.94934055648909 22.968521403896755 3 18.855026748283574 24.565764913968067 29.044098668427242 27.535109669321116 4 11.458964007135041 16.678567453023568 39.475813348609776 32.38665519123161 5 12.220263803123249 38.028111681449786 37.14831564650219 12.603308868924772 6 31.522337916792175 37.62737483683809 18.5849893758192 12.26529787055053 7 27.59218392431 31.668929711373366 22.748225165238544 17.990661199078094 8 23.930570407799536 38.37008073271675 20.877311848145215 16.822037011338498 9 26.087776523457006 15.702543255975513 20.62012895382411 37.58955126674337 10 14.140273642440906 29.661244663266718 35.27414456202824 20.92433713226413 11 33.833493887630084 22.839504497781636 25.077947980407856 18.249053634180427 12 23.291697082025134 24.91051778925345 32.38170573853013 19.416079390191285 13 28.76389859368958 22.633524866526013 25.84246338369277 22.760113156091638 14 19.73989851021682 23.177525711649615 28.07182980109088 29.010745977042685 15 22.324191799891185 32.66122959572017 23.125913282743518 21.888665321645124 16 20.82044908420634 28.669585664257387 29.55858189362167 20.951383357914604 17 20.63784751902959 29.686164366833555 28.784412694536034 20.89157541960082 18 20.671333984586994 28.17092695104743 31.01676978183002 20.140969282535547 19 23.260572524936194 27.24574989043137 27.68918058945889 21.804496995173555 20 23.01237006815274 28.085631209965523 28.790088749167253 20.111909972714482 21 21.947278230867024 27.496238326601443 27.473444417459593 23.083039025071944 22 22.1758190765774 29.408468438398465 27.750302231014395 20.665410254009732 23 21.111174428985837 28.937942150980568 28.490058641397408 21.460824778636187 24 22.81159730975411 27.40596418342191 29.124611788074617 20.657826718749366 25 21.740941443996103 28.172631533245234 28.8812788485804 21.205148174178266 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 197.0 1 197.0 2 254.0 3 311.0 4 311.0 5 311.0 6 507.0 7 703.0 8 703.0 9 703.0 10 1086.5 11 1470.0 12 1470.0 13 1470.0 14 2487.0 15 3504.0 16 3504.0 17 3504.0 18 6324.5 19 9145.0 20 9145.0 21 9145.0 22 14508.0 23 19871.0 24 19871.0 25 19871.0 26 28641.5 27 37412.0 28 37412.0 29 37412.0 30 49695.5 31 61979.0 32 61979.0 33 61979.0 34 70582.0 35 79185.0 36 79185.0 37 79185.0 38 84372.5 39 89560.0 40 89560.0 41 89560.0 42 92755.5 43 95951.0 44 95951.0 45 95951.0 46 96880.0 47 97809.0 48 97809.0 49 97809.0 50 92090.0 51 86371.0 52 86371.0 53 86371.0 54 74436.0 55 62501.0 56 62501.0 57 62501.0 58 52417.0 59 42333.0 60 42333.0 61 42333.0 62 34017.0 63 25701.0 64 25701.0 65 25701.0 66 19673.5 67 13646.0 68 13646.0 69 13646.0 70 9780.5 71 5915.0 72 5915.0 73 5915.0 74 4142.5 75 2370.0 76 2370.0 77 2370.0 78 1603.0 79 836.0 80 836.0 81 836.0 82 554.5 83 273.0 84 273.0 85 273.0 86 182.5 87 92.0 88 92.0 89 92.0 90 54.0 91 16.0 92 16.0 93 16.0 94 10.5 95 5.0 96 5.0 97 5.0 98 5.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22125424432383156 2 0.13701213167815443 3 0.1409461433798044 4 0.06986262159826687 5 0.023332759058061942 6 0.022111858874791262 7 0.29342301071272087 8 0.29070989919434154 9 0.020755303115601614 10 0.0389331502887429 11 0.01777088044538439 12 0.019398747356411967 13 0.017092602565789563 14 0.019127436204574036 15 0.016549980262113705 16 0.020348336387844716 17 0.020483991963763683 18 0.01641432468619474 19 0.024418003665413662 20 0.01899178062865507 21 0.016549980262113705 22 0.019263091780493 23 0.018856125052736105 24 0.017363913717627494 25 0.01668563583803267 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 737161.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.72023601975405 #Duplication Level Percentage of deduplicated Percentage of total 1 93.66841239671069 79.35610005844987 2 4.74051067264054 8.032343660805394 3 0.7595254673347656 1.9304153056684605 4 0.27352668675123126 0.9269298183706259 5 0.1434038300384834 0.6074603163498505 6 0.0888661812783537 0.4517258311245825 7 0.058388883568728095 0.3462703997810809 8 0.037881102289849214 0.2567436741347577 9 0.03165295718194105 0.24134814028594934 >10 0.16403290256262276 2.5648004588173086 >50 0.017941196837463612 1.0438896977160061 >100 0.013935574600026276 2.4462010593275783 >500 9.610741026534522E-4 0.5818989293036102 >1k 9.610741026534522E-4 1.2138726498649237 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2029 0.27524516353957956 No Hit GTCCTACAGTGTGCATTTCTCATTT 1585 0.21501408783155918 No Hit CTGTAGGACGTGGAATATGGCAAGA 1563 0.21202966516134197 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1549 0.21013048709847643 No Hit CTTTAGGACGTGAAATATGGCGAGG 1150 0.15600391230680952 No Hit CTGTAGGACCTGGAATATGGCGAGA 1069 0.14501581065737335 No Hit GTATCAACGCAGAGTACTTTTTTTT 1000 0.1356555759189648 No Hit GTACATGGGAAGCAGTGGTATCAAC 836 0.11340806146825455 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.356555759189648E-4 0.0 10 0.0 0.0 0.0 1.356555759189648E-4 0.0 11 0.0 0.0 0.0 1.356555759189648E-4 0.0 12 0.0 0.0 0.0 1.356555759189648E-4 0.0 13 0.0 0.0 0.0 1.356555759189648E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGACGT 40 0.0052046357 14.279116 4 TCCAACG 40 0.005342578 14.222933 18 TAGGACC 405 0.0 13.162641 4 TAGGACA 265 0.0 12.572805 4 GTAGGAC 940 0.0 12.47324 3 GGCGAGG 330 0.0 12.355275 19 TGTAGGA 1005 0.0 12.330461 2 GGTTATA 70 1.0439372E-4 12.270162 1 TAGAACC 70 1.0688287E-4 12.239242 4 GGACCTG 595 0.0 12.157591 6 GTCTAGG 55 0.0029627439 12.146221 1 AGTCGTC 55 0.0030393687 12.104896 8 AATCCCG 80 2.9177872E-5 11.852444 19 CTGTAGG 1000 0.0 11.833889 1 GCTATAC 105 2.5949157E-7 11.802012 3 GTCCTAC 1080 0.0 11.752593 1 GGAGTGT 65 7.88752E-4 11.7146015 6 CTTATAC 90 7.168548E-6 11.650704 3 AGGACCT 605 0.0 11.641991 5 TTTACAC 75 2.00651E-4 11.438873 3 >>END_MODULE