FastQCFastQC Report
Thu 26 May 2016
SRR1926936_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926936_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737161
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT19960.2707685295342537No Hit
CTGTAGGACGTGGAATATGGCAAGA19830.2690050070473072No Hit
GTCCTACAGTGTGCATTTCTCATTT16610.2253239116014005No Hit
GTATCAACGCAGAGTACTTTTTTTT16390.2223394889311833No Hit
GTCCTAAAGTGTGTATTTCTCATTT14920.20239811927109547No Hit
CTTTAGGACGTGAAATATGGCGAGG14420.19561534047514723No Hit
CTGTAGGACCTGGAATATGGCGAGA13470.18272806076284556No Hit
GTACATGGGAAGCAGTGGTATCAAC9410.12765189693974588No Hit
GGTATCAACGCAGAGTACTTTTTTT9300.12615968560463725No Hit
TATCAACGCAGAGTACTTTTTTTTT8580.11639248413847178No Hit
CCCATGTACTCTGCGTTGATACCAC8260.11205150570906491No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTGC500.00151079813.2852798
CTAATAC801.944658E-613.092453
TAGGACC5650.012.9739394
ATAGGAC1207.1122486E-1012.6957093
AGGACGT8900.012.3009145
GGACGTG8600.011.9551466
TGTAGGA12900.011.89432
GGCGAGG4000.011.86105219
ATTTAGA2500.011.8182721
TCCTAAA5450.011.7160452
CTGTAGG13050.011.6123181
GTAGGAC12300.011.6119293
GTGTTAA1001.8511619E-611.4370381
TTTAGAA2850.011.369382
GACGTGG4850.011.3521017
GACGTGA4100.011.344947
TAGGACG9350.011.3016254
GTCCTAA5150.011.2889851
CAATACT855.2020572E-511.1998094
GTATTAA1201.2152123E-711.1193421