##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926936_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 737161 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.81815370048063 31.0 30.0 33.0 26.0 34.0 2 30.18821261569725 31.0 30.0 34.0 26.0 34.0 3 30.259936431797126 31.0 30.0 34.0 26.0 34.0 4 33.85585645469578 35.0 35.0 37.0 30.0 37.0 5 33.812613526760096 37.0 35.0 37.0 30.0 37.0 6 33.76185934958578 37.0 35.0 37.0 29.0 37.0 7 33.76297308186407 37.0 35.0 37.0 29.0 37.0 8 33.79578273945583 37.0 35.0 37.0 29.0 37.0 9 35.35004429154554 39.0 35.0 39.0 30.0 39.0 10 35.23994486957395 39.0 35.0 39.0 29.0 39.0 11 35.27536182733487 39.0 35.0 39.0 30.0 39.0 12 35.17761113243918 39.0 35.0 39.0 29.0 39.0 13 35.19498454204712 39.0 35.0 39.0 29.0 39.0 14 36.28138222179415 40.0 36.0 41.0 29.0 41.0 15 36.27365663674557 40.0 36.0 41.0 29.0 41.0 16 36.25012717710242 40.0 36.0 41.0 29.0 41.0 17 36.22826221137581 40.0 36.0 41.0 29.0 41.0 18 36.20237777093471 40.0 36.0 41.0 29.0 41.0 19 36.2696534407002 40.0 37.0 41.0 29.0 41.0 20 36.23359483206518 40.0 37.0 41.0 29.0 41.0 21 36.17696405534205 40.0 37.0 41.0 29.0 41.0 22 36.092993253848206 40.0 37.0 41.0 29.0 41.0 23 36.021485130114044 40.0 37.0 41.0 28.0 41.0 24 35.959908351092906 40.0 36.0 41.0 28.0 41.0 25 35.847452591767606 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2639333119660776 1101 2 -0.06845804410888334 1101 3 0.14942839649858897 1101 4 0.2882950029467324 1101 5 0.15515053829424374 1101 6 0.09143378646808742 1101 7 0.22823652981369946 1101 8 0.11884153982042278 1101 9 0.3362747141882707 1101 10 0.43762749609745555 1101 11 0.35320478461892435 1101 12 0.364264559687129 1101 13 0.21458314492604558 1101 14 0.2830812634105371 1101 15 0.49186698260572825 1101 16 0.43955387598242623 1101 17 0.39358037488829467 1101 18 0.32056711281751404 1101 19 0.19955948014930414 1101 20 0.2967781796940159 1101 21 0.2469425666497429 1101 22 0.205252067815195 1101 23 0.4331091811258929 1101 24 0.37551736123703705 1101 25 0.3965719824335423 1102 1 -0.48227354212127693 1102 2 -0.4732021622250002 1102 3 -0.17463381759107222 1102 4 -0.4284823206387216 1102 5 -0.49522675843137165 1102 6 -0.5055688239719913 1102 7 -0.4213517592988367 1102 8 -0.5652868166095715 1102 9 -0.5695882838727826 1102 10 -0.5240300913858604 1102 11 -0.5808845411153172 1102 12 -0.43412804714366615 1102 13 -0.474620041321721 1102 14 -0.532810525034698 1102 15 -0.3876649847931546 1102 16 -0.5038532939144176 1102 17 -0.51391603680581 1102 18 -0.41284652022680035 1102 19 -0.39097662534269517 1102 20 -0.43853075036130207 1102 21 -0.4651963994145376 1102 22 -0.6108420087521154 1102 23 -0.2983210210782943 1102 24 -0.30614996293596874 1102 25 -0.39919507565924306 1103 1 -0.796028239557252 1103 2 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0.2510038430948285 2214 9 0.27173380272411407 2214 10 0.06720905796812104 2214 11 0.2219449949568073 2214 12 0.29785895067691115 2214 13 0.11488895532360033 2214 14 0.19154699026964295 2214 15 0.1100291367043269 2214 16 0.17121644031448824 2214 17 0.23718446230531498 2214 18 0.25987493708841214 2214 19 0.21667740362211418 2214 20 0.22442791490696123 2214 21 0.23488170292183952 2214 22 0.41237521244114106 2214 23 0.39179494229543366 2214 24 0.2498442054229102 2214 25 0.44609089046665673 2215 1 0.43542344871628913 2215 2 0.31198688778778205 2215 3 0.19369333995577875 2215 4 0.25147029307137814 2215 5 0.381151338638098 2215 6 0.3034611760132222 2215 7 0.11757417117073032 2215 8 0.23452827930849907 2215 9 0.06486329298508764 2215 10 0.1361794665736582 2215 11 0.03844288691472286 2215 12 0.15096847692564097 2215 13 0.16766224557669318 2215 14 0.26183213415500717 2215 15 0.20521938586045962 2215 16 0.14329177819226402 2215 17 0.13314700351392617 2215 18 0.21638753937114075 2215 19 -0.1234029777955925 2215 20 0.08473893320292802 2215 21 0.07450107326739897 2215 22 0.013102848150452928 2215 23 0.15952942093262834 2215 24 -0.10785178995384115 2215 25 -0.20371406638743395 2216 1 -0.2875987625148575 2216 2 -0.0962328536770336 2216 3 -1.7996564620704625 2216 4 -1.49567971808397 2216 5 -0.7357499486617485 2216 6 -0.20159770538799648 2216 7 -0.45978343690843104 2216 8 -0.23785753969536927 2216 9 -0.2200549504379623 2216 10 -0.19556031015817155 2216 11 -0.20516429443891582 2216 12 -0.2841074197355482 2216 13 -0.24839446120441266 2216 14 -0.3891897222555514 2216 15 -0.5114012706113868 2216 16 -0.37745447401528764 2216 17 -0.47638100446416587 2216 18 -0.3970045185613813 2216 19 -0.4662940666915887 2216 20 -0.35063667933299314 2216 21 -0.3912170755368294 2216 22 -0.5684507821748781 2216 23 -0.6336572173842328 2216 24 -0.47361939859748503 2216 25 -0.3670498099919257 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17982.0 3 19.0 4 284.0 5 1849.0 6 2521.0 7 1831.0 8 1392.0 9 1224.0 10 1097.0 11 1036.0 12 940.0 13 901.0 14 958.0 15 965.0 16 948.0 17 1011.0 18 1093.0 19 1324.0 20 1734.0 21 2217.0 22 2801.0 23 3460.0 24 4061.0 25 4835.0 26 5809.0 27 6371.0 28 7249.0 29 9059.0 30 11501.0 31 15102.0 32 19337.0 33 25777.0 34 34901.0 35 48995.0 36 72444.0 37 126341.0 38 297792.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.15357294684416 19.891914504073917 10.680743043643016 28.27376950543891 2 13.681687309865275 20.84908735332464 41.1678346371143 24.301390699695784 3 18.417346423745094 24.928903650878937 29.203232441644005 27.45051748373196 4 11.854126846102819 17.604159742579977 38.26884493346772 32.27286847784948 5 12.614296799960927 38.903103446404636 35.688005687028905 12.794594066605528 6 31.536808233479753 38.19472707746043 17.814387355221946 12.454077333837876 7 28.19078425173774 31.73450960013351 21.73262362944882 18.342082518679927 8 24.613879708469682 39.05160390516039 19.759834806722782 16.574681579647148 9 26.15324710916587 15.839858032672566 20.102541003512552 37.90435385464901 10 14.395213805079226 30.163881050575213 34.27840785760799 21.162497286737572 11 33.82913547553865 23.033907548546917 24.840392770803206 18.296564205111228 12 23.611827075951357 25.171198498011275 31.900710297413305 19.316264128624063 13 29.150376449840603 22.735535508376856 25.59641863935427 22.517669402428268 14 20.04948715409524 23.590504652307974 27.482734436574912 28.87727375702187 15 22.148167240958298 32.79431315625254 23.03320943104431 22.02431017174485 16 21.16057160376258 28.9677597896776 28.981189819223662 20.89047878733616 17 20.721146019860004 30.033371316946116 28.348526778447013 20.896955884746866 18 20.62163485734983 28.3331954153344 30.861707135425075 20.183462591890695 19 23.128660166938207 27.44496099213331 27.635014969633414 21.791363871295065 20 23.05942329397465 28.166239798576143 28.682415566938708 20.091921340510506 21 21.890755162401977 27.33251024339945 27.516620085203375 23.260114508995198 22 22.404467952048467 29.41216369287035 27.329307980210565 20.854060374870617 23 21.123122691305404 29.096572109951857 28.347886955188773 21.43241824355397 24 22.585703801476203 27.41477099303951 29.24164463394021 20.75788057154407 25 21.677203169090514 28.4351258953766 28.642826134143696 21.24484480138919 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 232.0 1 232.0 2 239.5 3 247.0 4 247.0 5 247.0 6 415.5 7 584.0 8 584.0 9 584.0 10 972.5 11 1361.0 12 1361.0 13 1361.0 14 2259.5 15 3158.0 16 3158.0 17 3158.0 18 5767.5 19 8377.0 20 8377.0 21 8377.0 22 13554.5 23 18732.0 24 18732.0 25 18732.0 26 27591.0 27 36450.0 28 36450.0 29 36450.0 30 48654.5 31 60859.0 32 60859.0 33 60859.0 34 69051.0 35 77243.0 36 77243.0 37 77243.0 38 82908.5 39 88574.0 40 88574.0 41 88574.0 42 92106.5 43 95639.0 44 95639.0 45 95639.0 46 97887.5 47 100136.0 48 100136.0 49 100136.0 50 94434.5 51 88733.0 52 88733.0 53 88733.0 54 76119.5 55 63506.0 56 63506.0 57 63506.0 58 53397.5 59 43289.0 60 43289.0 61 43289.0 62 34684.5 63 26080.0 64 26080.0 65 26080.0 66 19989.0 67 13898.0 68 13898.0 69 13898.0 70 10103.5 71 6309.0 72 6309.0 73 6309.0 74 4361.0 75 2413.0 76 2413.0 77 2413.0 78 1638.0 79 863.0 80 863.0 81 863.0 82 610.5 83 358.0 84 358.0 85 358.0 86 226.5 87 95.0 88 95.0 89 95.0 90 57.5 91 20.0 92 20.0 93 20.0 94 12.0 95 4.0 96 4.0 97 4.0 98 2.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12100477371971659 2 0.1140863393478494 3 0.018042191597222316 4 0.0 5 0.006104500916353416 6 0.33941025094924987 7 0.01899178062865507 8 0.011530723953112006 9 0.012209001832706831 10 0.005561878612677556 11 0.005290567460839626 12 6.782778795948239E-4 13 0.0014922113351086127 14 0.0 15 0.0028487670942982605 16 9.495890314327535E-4 17 1.356555759189648E-4 18 0.0010852446073517184 19 2.713111518379296E-4 20 0.0012209001832706832 21 0.013701213167815442 22 0.0 23 2.713111518379296E-4 24 0.0 25 0.005561878612677556 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 737161.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.09002774366509 #Duplication Level Percentage of deduplicated Percentage of total 1 92.06469391353248 75.57593277573916 2 6.010395292589097 9.867870326380663 3 0.956610732587649 2.3558460473402367 4 0.3310514950881844 1.0870410566548345 5 0.16804676612664768 0.6897481846784853 6 0.09849395902877117 0.48512230975531434 7 0.05850849586431745 0.33620748341193546 8 0.047848782511173145 0.3142326307074241 9 0.041875747894200545 0.3093823175779483 >10 0.1841254564418545 2.776305821989481 >50 0.01967109581156041 1.12084933213421 >100 0.015868258442883802 2.5573529087796003 >500 0.00165294357721102 0.955530276516462 >1k 0.0011570605040477138 1.5685785283343228 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1996 0.2707685295342537 No Hit CTGTAGGACGTGGAATATGGCAAGA 1983 0.2690050070473072 No Hit GTCCTACAGTGTGCATTTCTCATTT 1661 0.2253239116014005 No Hit GTATCAACGCAGAGTACTTTTTTTT 1639 0.2223394889311833 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1492 0.20239811927109547 No Hit CTTTAGGACGTGAAATATGGCGAGG 1442 0.19561534047514723 No Hit CTGTAGGACCTGGAATATGGCGAGA 1347 0.18272806076284556 No Hit GTACATGGGAAGCAGTGGTATCAAC 941 0.12765189693974588 No Hit GGTATCAACGCAGAGTACTTTTTTT 930 0.12615968560463725 No Hit TATCAACGCAGAGTACTTTTTTTTT 858 0.11639248413847178 No Hit CCCATGTACTCTGCGTTGATACCAC 826 0.11205150570906491 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGTGC 50 0.001510798 13.285279 8 CTAATAC 80 1.944658E-6 13.09245 3 TAGGACC 565 0.0 12.973939 4 ATAGGAC 120 7.1122486E-10 12.695709 3 AGGACGT 890 0.0 12.300914 5 GGACGTG 860 0.0 11.955146 6 TGTAGGA 1290 0.0 11.8943 2 GGCGAGG 400 0.0 11.861052 19 ATTTAGA 250 0.0 11.818272 1 TCCTAAA 545 0.0 11.716045 2 CTGTAGG 1305 0.0 11.612318 1 GTAGGAC 1230 0.0 11.611929 3 GTGTTAA 100 1.8511619E-6 11.437038 1 TTTAGAA 285 0.0 11.36938 2 GACGTGG 485 0.0 11.352101 7 GACGTGA 410 0.0 11.34494 7 TAGGACG 935 0.0 11.301625 4 GTCCTAA 515 0.0 11.288985 1 CAATACT 85 5.2020572E-5 11.199809 4 GTATTAA 120 1.2152123E-7 11.119342 1 >>END_MODULE