FastQCFastQC Report
Thu 26 May 2016
SRR1926935_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926935_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584043
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11700.20032771559628315No Hit
GTATCAACGCAGAGTACTTTTTTTT9320.15957729139806487No Hit
CTGTAGGACGTGGAATATGGCAAGA8030.1374898766015516No Hit
GTCCTACAGTGTGCATTTCTCATTT8000.13697621579233035No Hit
GTCCTAAAGTGTGTATTTCTCATTT7560.1294425239237522No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG5870.1005062983376224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGC807.346898E-915.4895313
GTATAAG551.8906777E-413.874311
GACTAAA551.8906777E-413.874311
CTTATAC551.9017627E-413.8647563
ACTGATC551.9424244E-413.8302348
TGACCAG551.9824036E-413.79706610
TAGGACC2850.013.0247584
TAAGGCT957.174822E-813.0247574
TGTAGGA5250.012.3451722
GGCTAAG701.0482038E-412.2638971
TACTAGG701.055818E-412.2543982
TAGGGTG550.003026961612.1108125
GACTGAC550.003026961612.1108127
CGTGATT550.00310378112.07036418
GTATCAA5350.011.945521
TTATACA1209.680662E-911.89761454
AGGACGT3600.011.8945485
AGGACCT3950.011.804215
GTAGGAC5250.011.8015473
GGACGTG3550.011.7940316
Produced by FastQC (version 0.11.5)