##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926935_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 584043 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.397736125593493 31.0 28.0 31.0 22.0 34.0 2 28.360742274113377 31.0 28.0 31.0 20.0 34.0 3 28.087065507163 31.0 28.0 31.0 16.0 34.0 4 32.01504170069669 35.0 32.0 37.0 20.0 37.0 5 32.05388473109001 35.0 33.0 37.0 20.0 37.0 6 31.570547031639794 35.0 32.0 37.0 17.0 37.0 7 31.42875096525427 35.0 32.0 37.0 17.0 37.0 8 31.126954693404425 35.0 32.0 37.0 15.0 37.0 9 33.33303883446938 37.0 33.0 39.0 17.0 39.0 10 33.522278325397274 38.0 34.0 39.0 18.0 39.0 11 33.62229150935804 38.0 34.0 39.0 21.0 39.0 12 33.54029754658475 38.0 34.0 39.0 19.0 39.0 13 33.63111620206046 38.0 34.0 39.0 21.0 39.0 14 34.66134856508853 39.0 34.0 41.0 20.0 41.0 15 34.254654194982216 39.0 34.0 41.0 17.0 41.0 16 34.34253642283188 39.0 34.0 41.0 17.0 41.0 17 34.56904200546877 39.0 34.0 41.0 19.0 41.0 18 34.601632071611164 39.0 34.0 41.0 19.0 41.0 19 34.00694126973528 39.0 34.0 41.0 11.0 41.0 20 34.31294956022074 39.0 34.0 41.0 18.0 41.0 21 34.294207789494955 39.0 34.0 41.0 17.0 41.0 22 34.30955426227178 39.0 34.0 41.0 16.0 41.0 23 34.30774788842602 39.0 34.0 41.0 10.0 41.0 24 33.05198932270398 39.0 32.0 41.0 2.0 41.0 25 33.61297027787337 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.2506672234517744 1101 2 -0.5327860662060111 1101 3 -1.2172203620269961 1101 4 -0.5596197585806983 1101 5 -0.5108233290905275 1101 6 -2.3937395582377157 1101 7 -0.6556844171046485 1101 8 -0.1957105538620887 1101 9 -0.19923821665886265 1101 10 -8.842269302088134 1101 11 -4.1233325142911035 1101 12 -1.500513618255333 1101 13 -0.6966665687611737 1101 14 -0.707464655622168 1101 15 -2.137624944733105 1101 16 -0.4813604781639995 1101 17 -0.34653942659056014 1101 18 -0.14847142240831346 1101 19 -0.6077122686664964 1101 20 -0.07846022708835676 1101 21 -0.14683088261084976 1101 22 -0.020200194732908017 1101 23 -0.027450646087878283 1101 24 -0.35550173720965006 1101 25 -0.21525591136838784 1102 1 -0.5844737907437683 1102 2 -0.8391513082645119 1102 3 -0.9832157590350512 1102 4 -0.9391110209244786 1102 5 -0.7449282880115007 1102 6 -1.1582991964211402 1102 7 -0.6283273908515348 1102 8 -0.6126131684685596 1102 9 -0.45704724601631597 1102 10 -0.290888404504706 1102 11 -0.6414674997060459 1102 12 -0.4940065398171356 1102 13 -0.5567757560119588 1102 14 -0.8024829873052042 1102 15 -0.151427230172132 1102 16 -0.3798604915447896 1102 17 -0.3592685726285083 1102 18 -0.6467185388479493 1102 19 -0.5010700442438818 1102 20 -0.4016866033026645 1102 21 -0.5741009337254681 1102 22 -0.6319445145873246 1102 23 -0.7334840445410578 1102 24 -3.8126577839888967 1102 25 -2.239907341774895 1103 1 -0.03826277216473528 1103 2 -0.1736728122827742 1103 3 0.17203631625407212 1103 4 0.01712847900134662 1103 5 -0.03288258955024048 1103 6 0.6351154778209462 1103 7 0.6687152803806988 1103 8 0.5790618848922016 1103 9 0.47077906989274254 1103 10 0.3718421961697089 1103 11 0.20712828035006225 1103 12 0.056933104052419026 1103 13 0.07111832171231214 1103 14 0.07656284079897802 1103 15 0.6392502409622693 1103 16 0.47466010363650213 1103 17 0.04343842501519646 1103 18 0.2145461612558961 1103 19 -1.2004762262064048 1103 20 -0.17394141445836908 1103 21 -0.06453609288554674 1103 22 0.037535537109455674 1103 23 0.05468343272799103 1103 24 1.4578588764629288 1103 25 0.6577054910031137 1104 1 -0.6150795356617316 1104 2 -0.5189125442742331 1104 3 -1.3096104409446987 1104 4 -0.5960726570364265 1104 5 -0.42323779121789684 1104 6 -1.7028793290879811 1104 7 -0.4760679938331016 1104 8 -0.12115036928381073 1104 9 -0.11142326686729831 1104 10 -0.12812048585363556 1104 11 -0.2493504477485402 1104 12 0.04623266527158165 1104 13 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16 -1.1510044270061002 2213 17 -0.6040003612248555 2213 18 -0.38002694535845194 2213 19 0.20443406444675105 2213 20 -1.364523856560396 2213 21 -0.304686750363274 2213 22 -0.46746378507844355 2213 23 -0.39518228720005055 2213 24 -0.554642176893708 2213 25 -0.3136798470052895 2214 1 0.340494854606046 2214 2 0.3072393512821705 2214 3 0.1574987390965994 2214 4 0.2872113695281904 2214 5 0.5405865100904137 2214 6 0.2788428110522574 2214 7 0.6739055774317784 2214 8 -2.626344565786926 2214 9 -1.3552125141147364 2214 10 -0.6424225196570816 2214 11 -0.33203265919453884 2214 12 -1.3866895927903329 2214 13 -0.37749180571393026 2214 14 -0.2685747109705332 2214 15 -1.612747875108191 2214 16 -0.4508060443563693 2214 17 -0.3917481112949375 2214 18 -0.24508163216197687 2214 19 0.5393742220573046 2214 20 -1.3970577518525928 2214 21 -0.5269719853022536 2214 22 -0.12791689663566075 2214 23 -0.348823903089567 2214 24 -1.7486425785997746 2214 25 -1.1409430149624669 2215 1 0.039434418272858096 2215 2 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-2.0687932264586593 2216 14 -2.115368749414209 2216 15 -3.164412375061765 2216 16 -2.4509453330554116 2216 17 -2.6479232436192 2216 18 -2.202935193309713 2216 19 -1.543595413006301 2216 20 -2.2732997427523998 2216 21 -5.107132631601818 2216 22 -3.682668962751361 2216 23 -3.765587298019458 2216 24 -3.277219976686876 2216 25 -2.775240535623624 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31062.0 3 3.0 4 282.0 5 1597.0 6 2073.0 7 1756.0 8 1296.0 9 1175.0 10 1066.0 11 1014.0 12 988.0 13 997.0 14 991.0 15 1051.0 16 1114.0 17 1166.0 18 1416.0 19 1746.0 20 2207.0 21 2955.0 22 3554.0 23 4448.0 24 5208.0 25 5899.0 26 6859.0 27 7455.0 28 8287.0 29 9904.0 30 11800.0 31 14203.0 32 18657.0 33 24392.0 34 33056.0 35 46619.0 36 67709.0 37 109152.0 38 150886.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.39441984968599 18.873331274237277 12.251964720820894 25.480284155255845 2 13.787776697647544 20.487327329180832 43.100282376903735 22.624613596267892 3 19.07455383090753 24.511567219838042 29.097991746150402 27.315887203104023 4 10.936415709563944 16.74157284118577 40.517015381610264 31.80499606764002 5 11.974193725177216 38.41620581994181 37.308289760553805 12.301310694327174 6 31.047020440318896 38.08955377251047 18.97361683179339 11.889808955377251 7 27.335902164618126 32.08978546137652 22.57892802807856 17.995384345926787 8 23.657501764242287 38.33737120172802 21.169533810780507 16.835593223249194 9 25.880700792930416 15.941326574300835 20.59820862804199 37.579764004726755 10 13.15313000505323 30.44425601891107 36.07458225202772 20.32803172400798 11 34.07583482181848 23.397273974315283 24.44706273215659 18.07982847170965 12 22.104340171633982 25.75468773815457 32.971124320751066 19.169847769460386 13 28.35129493039417 22.79533027018603 26.10839294368684 22.744981855732956 14 19.41899376282286 23.16729403786148 29.00544254501488 28.408269654300778 15 22.20110114995676 32.34777843424354 23.70109686865833 21.750023547141378 16 20.615954252988917 29.820330977364545 29.083227294757556 20.48048747488898 17 20.41451735547381 29.83500825472506 28.78193140015208 20.968542989649055 18 19.932869240583283 29.278943718050808 30.750511615162644 20.037675426203258 19 22.302107756413438 28.12151583086569 28.423627012575974 21.15274940014489 20 22.05654179839088 28.534975561804487 29.65567318922733 19.752809450577303 21 21.904380259548102 27.90347605396463 28.17131270785597 22.02083097863129 22 21.32583003522737 29.548688949361303 28.722378540932763 20.40310247447857 23 20.96575038661184 29.06379138202388 28.632738444887046 21.337719786477233 24 21.64744363670614 28.207934102254534 29.713752386823877 20.430869874215453 25 21.304487805212197 28.62118923584353 29.258928173881472 20.8153947850628 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 155.0 1 155.0 2 200.5 3 246.0 4 246.0 5 246.0 6 429.5 7 613.0 8 613.0 9 613.0 10 1013.0 11 1413.0 12 1413.0 13 1413.0 14 2387.0 15 3361.0 16 3361.0 17 3361.0 18 5621.5 19 7882.0 20 7882.0 21 7882.0 22 12412.5 23 16943.0 24 16943.0 25 16943.0 26 23841.0 27 30739.0 28 30739.0 29 30739.0 30 39898.5 31 49058.0 32 49058.0 33 49058.0 34 56550.5 35 64043.0 36 64043.0 37 64043.0 38 69131.5 39 74220.0 40 74220.0 41 74220.0 42 76783.5 43 79347.0 44 79347.0 45 79347.0 46 79236.0 47 79125.0 48 79125.0 49 79125.0 50 73969.5 51 68814.0 52 68814.0 53 68814.0 54 58766.5 55 48719.0 56 48719.0 57 48719.0 58 39660.5 59 30602.0 60 30602.0 61 30602.0 62 23539.0 63 16476.0 64 16476.0 65 16476.0 66 12124.0 67 7772.0 68 7772.0 69 7772.0 70 5406.5 71 3041.0 72 3041.0 73 3041.0 74 2028.5 75 1016.0 76 1016.0 77 1016.0 78 659.0 79 302.0 80 302.0 81 302.0 82 199.5 83 97.0 84 97.0 85 97.0 86 71.0 87 45.0 88 45.0 89 45.0 90 26.5 91 8.0 92 8.0 93 8.0 94 5.0 95 2.0 96 2.0 97 2.0 98 3.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21625120068214154 2 0.1335518103975221 3 0.13697621579233035 4 0.07259739436993509 5 0.026367921540023594 6 0.025340599921581115 7 0.28713639235467253 8 0.2808012423742773 9 0.022772295875474923 10 0.04417482959302654 11 0.0198615512898879 12 0.02157375398729203 13 0.01917667021092625 14 0.02140253371755162 15 0.018491789131964597 16 0.02328595668469616 17 0.023114736414955747 18 0.018491789131964597 19 0.026025481000542767 20 0.02071765263858997 21 0.01797812832274336 22 0.02088887290833038 23 0.021231313447811206 24 0.018491789131964597 25 0.01797812832274336 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 584043.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.83931543276456 #Duplication Level Percentage of deduplicated Percentage of total 1 95.62393953080104 85.90789266431239 2 3.4691377008462423 6.233299123720423 3 0.41378149537171754 1.1152153884882225 4 0.14517856554185948 0.521709717751656 5 0.08252293719016852 0.37069020923328844 6 0.0479570116384898 0.2585055057481828 7 0.035388496965634494 0.2225494839110949 8 0.02577421325249516 0.1852430139537803 9 0.02213682221507867 0.17898812582935344 >10 0.11378195828714711 1.9651221086849253 >50 0.011058677009152616 0.6738685055069165 >100 0.007435939680794583 1.1375517564995221 >500 0.0017159860801293024 1.0289524772099272 >1k 1.9066512001436693E-4 0.2004119191502605 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1170 0.20032771559628315 No Hit GTATCAACGCAGAGTACTTTTTTTT 932 0.15957729139806487 No Hit CTGTAGGACGTGGAATATGGCAAGA 803 0.1374898766015516 No Hit GTCCTACAGTGTGCATTTCTCATTT 800 0.13697621579233035 No Hit GTCCTAAAGTGTGTATTTCTCATTT 756 0.1294425239237522 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 587 0.1005062983376224 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.7122026974041295E-4 0.0 9 0.0 0.0 0.0 3.424405394808259E-4 0.0 10 0.0 0.0 0.0 3.424405394808259E-4 0.0 11 0.0 0.0 0.0 3.424405394808259E-4 0.0 12 0.0 0.0 0.0 5.136608092212388E-4 0.0 13 0.0 0.0 0.0 5.136608092212388E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAAGGC 80 7.346898E-9 15.489531 3 GTATAAG 55 1.8906777E-4 13.87431 1 GACTAAA 55 1.8906777E-4 13.87431 1 CTTATAC 55 1.9017627E-4 13.864756 3 ACTGATC 55 1.9424244E-4 13.830234 8 TGACCAG 55 1.9824036E-4 13.797066 10 TAGGACC 285 0.0 13.024758 4 TAAGGCT 95 7.174822E-8 13.024757 4 TGTAGGA 525 0.0 12.345172 2 GGCTAAG 70 1.0482038E-4 12.263897 1 TACTAGG 70 1.055818E-4 12.254398 2 TAGGGTG 55 0.0030269616 12.110812 5 GACTGAC 55 0.0030269616 12.110812 7 CGTGATT 55 0.003103781 12.070364 18 GTATCAA 535 0.0 11.94552 1 TTATACA 120 9.680662E-9 11.8976145 4 AGGACGT 360 0.0 11.894548 5 AGGACCT 395 0.0 11.80421 5 GTAGGAC 525 0.0 11.801547 3 GGACGTG 355 0.0 11.794031 6 >>END_MODULE