FastQCFastQC Report
Thu 26 May 2016
SRR1926935_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926935_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584043
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15430.2641928762094572No Hit
CTGTAGGACGTGGAATATGGCAAGA10870.18611643320782886No Hit
GTCCTACAGTGGACATTTCTAAATT10220.17498711567470204No Hit
GGTATCAACGCAGAGTACTTTTTTT9870.1689944062337876No Hit
GTCCTACAGTGTGCATTTCTCATTT8640.14793431305571678No Hit
CACACACACACACACACACACACAC8270.14159916307532153No Hit
TATCAACGCAGAGTACTTTTTTTTT8010.13714743606207078No Hit
CTTTAGGACGTGAAATATGGCGAGG7060.12088151043673154No Hit
GTCCTAAAGTGTGTATTTCTCATTT6800.11642978342348081No Hit
CTGTAGGACCTGGAATATGGCGAGA6380.10923853209438346No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG6310.10803999020620057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG801.233766E-714.296311
GGTATCA4050.014.1198131
GTCTAGG500.00146140613.3432241
AAGGTGT500.001474722613.3271776
GCTAGAC655.3292475E-513.182993
TAGAACC655.333546E-513.1818574
CCTCTAG603.974879E-412.7078311
TCTAGAC1051.9233994E-812.6947323
TAGGACC2800.012.5803044
GTACTGA701.06809304E-412.2392446
TGTAGGA6700.012.2326062
TCACGTC701.0993361E-412.2015148
AGGACGT5000.012.1858955
TTAGGCT550.003016044912.1166564
AGGACCT4600.012.0037695
CTAGACT802.7975806E-511.9002884
GGACGTG4750.011.6237046
GTAGGAC6150.011.6110343
TAGGACG5250.011.6056144
CTCGAAC907.5396456E-611.59698418