##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926935_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 584043 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.936814583857696 31.0 30.0 33.0 26.0 34.0 2 30.21537455290107 31.0 30.0 34.0 26.0 34.0 3 30.261595464717495 31.0 30.0 34.0 26.0 34.0 4 33.84625618319199 35.0 35.0 37.0 28.0 37.0 5 33.890821395000025 37.0 35.0 37.0 30.0 37.0 6 33.85506717827283 37.0 35.0 37.0 30.0 37.0 7 33.85229340990304 37.0 35.0 37.0 29.0 37.0 8 33.89444784031313 37.0 35.0 37.0 30.0 37.0 9 35.41900339529795 39.0 35.0 39.0 30.0 39.0 10 35.31777283521932 39.0 35.0 39.0 30.0 39.0 11 35.352359329706886 39.0 35.0 39.0 30.0 39.0 12 35.25640235393627 39.0 35.0 39.0 30.0 39.0 13 35.27468354213645 39.0 35.0 39.0 30.0 39.0 14 36.374028967045234 40.0 36.0 41.0 30.0 41.0 15 36.369238223897895 40.0 36.0 41.0 30.0 41.0 16 36.34589405232149 40.0 36.0 41.0 29.0 41.0 17 36.336459815458795 40.0 36.0 41.0 30.0 41.0 18 36.302577036279864 40.0 36.0 41.0 29.0 41.0 19 36.38756392936821 40.0 37.0 41.0 29.0 41.0 20 36.34690425191296 40.0 37.0 41.0 29.0 41.0 21 36.301000097595555 40.0 37.0 41.0 29.0 41.0 22 36.21833495136488 40.0 37.0 41.0 29.0 41.0 23 36.14811923094704 40.0 37.0 41.0 29.0 41.0 24 36.07236624700579 40.0 37.0 41.0 29.0 41.0 25 35.962276750170794 40.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.47836943593110703 1101 2 -0.193347988374569 1101 3 -0.22192626576323704 1101 4 -0.33110518484544826 1101 5 -0.35003151759939044 1101 6 -0.3872632011374364 1101 7 -0.4118483612851165 1101 8 -0.5814231784714039 1101 9 -0.6261728095398027 1101 10 -0.3957564649212486 1101 11 -0.46796758020727935 1101 12 -0.19741567809431615 1101 13 -0.44007036750406314 1101 14 -0.41905224903374005 1101 15 -0.2397305057914636 1101 16 -0.23478474982942998 1101 17 -0.1607328142350113 1101 18 -0.2845542381142465 1101 19 -0.09319574072500814 1101 20 -0.11249931887807207 1101 21 -0.0923702786279108 1101 22 -0.3760363178082784 1101 23 -0.2902810262303106 1101 24 -0.2856890812489823 1101 25 -0.2764193004659248 1102 1 -0.24469266230732245 1102 2 -0.4030249760910003 1102 3 -0.2890068448838541 1102 4 -0.4055211133385015 1102 5 -0.30164256478005314 1102 6 -0.19651334163573608 1102 7 -0.4267331526785938 1102 8 -0.22465918885490055 1102 9 -0.3651952089831596 1102 10 -0.28448983928900873 1102 11 -0.3839335394761534 1102 12 -0.433704382030939 1102 13 -0.4244429422357783 1102 14 -0.46469680171391303 1102 15 -0.5068539034418009 1102 16 -0.34388234931560646 1102 17 -0.26138312065511116 1102 18 -0.4469301312820093 1102 19 -0.4402198529095571 1102 20 -0.5325450811817092 1102 21 -0.5537104986081047 1102 22 -0.6908421625375851 1102 23 -0.5155934676893565 1102 24 -0.7829941900348132 1102 25 -0.4457799662940616 1103 1 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5 0.4615619451176727 2214 6 0.5371078941900223 2214 7 0.350125176021578 2214 8 0.25688358923175514 2214 9 0.13037635073290232 2214 10 -0.011555690519273298 2214 11 0.1618183902894259 2214 12 0.3935670522665191 2214 13 0.28300847421862585 2214 14 0.26371374379235135 2214 15 0.11885561673508249 2214 16 0.17721254213911664 2214 17 0.07586361781478246 2214 18 0.23292919300911308 2214 19 0.09485210116396559 2214 20 -0.04580454252821653 2214 21 0.031037565161433633 2214 22 0.20818939898864386 2214 23 0.2632560429195081 2214 24 0.04970774037725789 2214 25 0.2530602348928923 2215 1 0.6151362667785598 2215 2 0.39870334772229654 2215 3 0.39103422511476893 2215 4 0.6236033563381866 2215 5 0.4685485428235765 2215 6 0.46406235683816277 2215 7 0.07843478351920652 2215 8 0.23537346782007518 2215 9 0.1043864541431887 2215 10 0.2167379728876 2215 11 0.1505176302191913 2215 12 0.22056132370900627 2215 13 0.19573257870076333 2215 14 0.21735141381550704 2215 15 0.19460753491206617 2215 16 0.22552930904782897 2215 17 0.3270303652305486 2215 18 0.24158220536218522 2215 19 0.03380372062068915 2215 20 0.18876183477892283 2215 21 0.19147009234096402 2215 22 0.10552179830685304 2215 23 0.12138552333759378 2215 24 0.31271837560012017 2215 25 0.22786651419015413 2216 1 -0.19347130024015868 2216 2 -0.07920710811318088 2216 3 -1.6625422565377903 2216 4 -1.2040486789834475 2216 5 -0.34734556718616716 2216 6 -0.16501161551375532 2216 7 -0.2571155148503692 2216 8 -0.10587061264399722 2216 9 -0.17836865808871494 2216 10 -0.29318583259374265 2216 11 -0.1804603728269285 2216 12 -0.12512084818848734 2216 13 -0.12399310438663491 2216 14 -0.2382479053858475 2216 15 -0.4903119011403021 2216 16 -0.4720469062776331 2216 17 -0.5291020243061197 2216 18 -0.4325457814546212 2216 19 -0.40597202363871077 2216 20 -0.365843543363944 2216 21 -0.2068907475881261 2216 22 -0.3097091988384548 2216 23 -0.6028410877332888 2216 24 -0.7339304804417566 2216 25 -0.5853996963913559 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13306.0 3 15.0 4 197.0 5 1407.0 6 1914.0 7 1426.0 8 1146.0 9 987.0 10 919.0 11 783.0 12 763.0 13 714.0 14 712.0 15 704.0 16 728.0 17 757.0 18 820.0 19 1054.0 20 1307.0 21 1701.0 22 2215.0 23 2604.0 24 3203.0 25 3769.0 26 4568.0 27 5085.0 28 5806.0 29 6996.0 30 9115.0 31 11738.0 32 15596.0 33 20294.0 34 27629.0 35 38529.0 36 57260.0 37 99940.0 38 238336.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.793050489537336 19.614232235416722 10.393926720236953 28.198790554808983 2 13.90668200197805 21.239691945361955 40.955051653831056 23.898574398828938 3 18.792608309230367 24.666368697480788 29.122549246257833 27.418473747031015 4 11.420734432225025 17.82608472321387 38.8829247161596 31.870256128401504 5 12.275925364675102 39.1693335593557 35.956391381698694 12.598349694270505 6 30.875903003856823 38.986745921987335 18.076913165602964 12.060437908552874 7 28.064262557863533 32.072880220990314 21.61180441740864 18.251052803737515 8 24.298777355388886 39.2689818144457 19.852220966471453 16.580019863693963 9 25.840053015423447 16.061138975411872 20.130620688001834 37.968187321162844 10 13.440658608709649 31.160630047995014 35.05365407632286 20.34505726697248 11 33.95044922406752 23.501773095185502 24.236867023854167 18.310910656892812 12 22.23508281096704 26.12196746461293 32.34344849077376 19.299501233646268 13 28.510131190093794 22.80176974785887 25.900369496505366 22.787729565541966 14 19.543081588170736 23.59706391481449 28.528378903608125 28.33147559340665 15 21.905440274778996 32.498848523970395 23.663217660645888 21.932493540604725 16 20.73857702014581 30.044414614017484 28.639721115947143 20.57728724988956 17 20.191698542228128 30.1502289218926 28.490416785094226 21.16765575078505 18 19.862989070972755 29.391600932126337 30.504707717437647 20.240702279463257 19 21.969310426304958 28.367309200365725 28.544180041846307 21.11920033148301 20 21.95582159471681 28.749693942719773 29.367287570564393 19.92719689199902 21 21.618326466730366 27.8975865859798 28.379104978544007 22.104981968745825 22 21.33130608533961 29.67144542439512 28.565191261602312 20.43205722866296 23 20.87620260836959 29.115664428817738 28.687442534196965 21.320690428615702 24 21.34336459255429 28.111040149578542 29.886086764456604 20.659508493410566 25 21.220013355935684 28.777760654783307 29.167137549014573 20.835088440266432 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 205.0 1 205.0 2 214.5 3 224.0 4 224.0 5 224.0 6 405.0 7 586.0 8 586.0 9 586.0 10 962.5 11 1339.0 12 1339.0 13 1339.0 14 2205.0 15 3071.0 16 3071.0 17 3071.0 18 5231.5 19 7392.0 20 7392.0 21 7392.0 22 11736.5 23 16081.0 24 16081.0 25 16081.0 26 22881.0 27 29681.0 28 29681.0 29 29681.0 30 38824.0 31 47967.0 32 47967.0 33 47967.0 34 55312.0 35 62657.0 36 62657.0 37 62657.0 38 67856.0 39 73055.0 40 73055.0 41 73055.0 42 76568.0 43 80081.0 44 80081.0 45 80081.0 46 80333.5 47 80586.0 48 80586.0 49 80586.0 50 75785.0 51 70984.0 52 70984.0 53 70984.0 54 60086.0 55 49188.0 56 49188.0 57 49188.0 58 40351.0 59 31514.0 60 31514.0 61 31514.0 62 24314.5 63 17115.0 64 17115.0 65 17115.0 66 12483.0 67 7851.0 68 7851.0 69 7851.0 70 5445.0 71 3039.0 72 3039.0 73 3039.0 74 2009.5 75 980.0 76 980.0 77 980.0 78 659.0 79 338.0 80 338.0 81 338.0 82 208.0 83 78.0 84 78.0 85 78.0 86 50.0 87 22.0 88 22.0 89 22.0 90 15.5 91 9.0 92 9.0 93 9.0 94 4.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10872487128516223 2 0.10958097263386428 3 0.015409824276637165 4 0.0 5 0.005136608092212388 6 0.33524928815172855 7 0.02157375398729203 8 0.010786876993646015 9 0.010615656723905603 10 0.004109286473769911 11 0.006163929710654866 12 3.424405394808259E-4 13 8.561013487020647E-4 14 0.0 15 0.0017122026974041294 16 1.7122026974041295E-4 17 1.7122026974041295E-4 18 3.424405394808259E-4 19 1.7122026974041295E-4 20 3.424405394808259E-4 21 0.009759555375203538 22 0.0 23 0.0 24 3.424405394808259E-4 25 0.005650268901433627 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 584043.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.94194262920206 #Duplication Level Percentage of deduplicated Percentage of total 1 94.60063287794374 83.19363429238335 2 4.323509730068982 7.604356892770466 3 0.517033697117488 1.364068431878111 4 0.16883439296902375 0.5939049800127217 5 0.08798836387810052 0.38689338241026355 6 0.0586534116096442 0.3094856975269383 7 0.04418276097059188 0.2719862481332877 8 0.028882591855946216 0.20319929887826246 9 0.020903435774268113 0.1654459874652507 >10 0.12558398642212476 2.0900064855669407 >50 0.012482538461254132 0.731024319704252 >100 0.008971755081633262 1.3205351088047332 >500 0.0017553433854530036 1.139066587518221 >1k 5.851144618176678E-4 0.6263922869472284 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1543 0.2641928762094572 No Hit CTGTAGGACGTGGAATATGGCAAGA 1087 0.18611643320782886 No Hit GTCCTACAGTGGACATTTCTAAATT 1022 0.17498711567470204 No Hit GGTATCAACGCAGAGTACTTTTTTT 987 0.1689944062337876 No Hit GTCCTACAGTGTGCATTTCTCATTT 864 0.14793431305571678 No Hit CACACACACACACACACACACACAC 827 0.14159916307532153 No Hit TATCAACGCAGAGTACTTTTTTTTT 801 0.13714743606207078 No Hit CTTTAGGACGTGAAATATGGCGAGG 706 0.12088151043673154 No Hit GTCCTAAAGTGTGTATTTCTCATTT 680 0.11642978342348081 No Hit CTGTAGGACCTGGAATATGGCGAGA 638 0.10923853209438346 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 631 0.10803999020620057 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 1.7122026974041295E-4 0.0 11 0.0 0.0 0.0 1.7122026974041295E-4 0.0 12 0.0 0.0 0.0 1.7122026974041295E-4 0.0 13 0.0 0.0 0.0 1.7122026974041295E-4 1.7122026974041295E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAG 80 1.233766E-7 14.29631 1 GGTATCA 405 0.0 14.119813 1 GTCTAGG 50 0.001461406 13.343224 1 AAGGTGT 50 0.0014747226 13.327177 6 GCTAGAC 65 5.3292475E-5 13.18299 3 TAGAACC 65 5.333546E-5 13.181857 4 CCTCTAG 60 3.974879E-4 12.707831 1 TCTAGAC 105 1.9233994E-8 12.694732 3 TAGGACC 280 0.0 12.580304 4 GTACTGA 70 1.06809304E-4 12.239244 6 TGTAGGA 670 0.0 12.232606 2 TCACGTC 70 1.0993361E-4 12.201514 8 AGGACGT 500 0.0 12.185895 5 TTAGGCT 55 0.0030160449 12.116656 4 AGGACCT 460 0.0 12.003769 5 CTAGACT 80 2.7975806E-5 11.900288 4 GGACGTG 475 0.0 11.623704 6 GTAGGAC 615 0.0 11.611034 3 TAGGACG 525 0.0 11.605614 4 CTCGAAC 90 7.5396456E-6 11.596984 18 >>END_MODULE