FastQCFastQC Report
Thu 26 May 2016
SRR1926934_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926934_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667401
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT18580.27839334972527763No Hit
CTGTAGGACGTGGAATATGGCAAGA16310.24438081453279212No Hit
GTCCTAAAGTGTGTATTTCTCATTT14850.22250491084070895No Hit
GTCCTACAGTGTGCATTTCTCATTT12550.1880428707778382No Hit
CTTTAGGACGTGAAATATGGCGAGG12040.18040128798128863No Hit
GTATCAACGCAGAGTACTTTTTTTT9930.14878611209752457No Hit
CTGTAGGACCTGGAATATGGCGAGA8070.1209168101336378No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCC250.006087946718.9677729
GGGCTAA400.00512597114.3116371
TGTAGAA2200.012.9958822
TCCTATA1700.012.8939532
GTCTTAC603.9404057E-412.7214561
GTAAGAC603.9807052E-412.7061273
TACTTAG604.0213641E-412.6908357
TAGGACC3450.012.4224244
TAGAGTG1103.695459E-812.1148925
TAGGACT951.0058502E-612.0301374
CCTACAC907.189159E-611.6472843
CTAGAGT1406.493792E-1011.5646854
TGTAGGA10050.011.4743082
TAGGACA2750.011.428634
TTAGACA752.0227337E-411.4286294
ACTGTTC1850.011.3103478
TTAGGAC5250.011.2539983
TGGCGAG8200.011.21706318
GGACCTG5350.011.20826156
TAGACAT855.2058662E-511.1986395
Produced by FastQC (version 0.11.5)