##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926934_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 667401 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.420511806245422 31.0 28.0 31.0 22.0 34.0 2 28.428406610118955 31.0 28.0 31.0 22.0 34.0 3 28.114273128149343 31.0 28.0 31.0 16.0 34.0 4 32.01034760211627 35.0 32.0 37.0 20.0 37.0 5 32.030400014384156 35.0 32.0 37.0 20.0 37.0 6 31.547678232426982 35.0 32.0 37.0 17.0 37.0 7 31.44467269302863 35.0 32.0 37.0 17.0 37.0 8 31.13571451046672 35.0 32.0 37.0 16.0 37.0 9 33.30873043342758 37.0 33.0 39.0 17.0 39.0 10 33.476732878734076 37.0 34.0 39.0 18.0 39.0 11 33.63207427019138 38.0 34.0 39.0 22.0 39.0 12 33.50520301887471 38.0 34.0 39.0 18.0 39.0 13 33.60842581896041 38.0 34.0 39.0 21.0 39.0 14 34.63601343120553 39.0 34.0 41.0 21.0 41.0 15 34.21908118207794 39.0 34.0 41.0 17.0 41.0 16 34.303805358397724 39.0 34.0 41.0 17.0 41.0 17 34.52933693536569 39.0 34.0 41.0 19.0 41.0 18 34.58067188991326 39.0 34.0 41.0 19.0 41.0 19 33.95660180311387 39.0 33.0 41.0 11.0 41.0 20 34.24320461012195 39.0 34.0 41.0 18.0 41.0 21 34.22388339244323 39.0 34.0 41.0 16.0 41.0 22 34.254376304500596 39.0 34.0 41.0 16.0 41.0 23 34.24575180438747 39.0 34.0 41.0 10.0 41.0 24 32.95695990866061 39.0 32.0 41.0 2.0 41.0 25 33.52935641391008 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.2796111310990348 1101 2 -0.26157215403869927 1101 3 -0.9354017927168989 1101 4 -0.48245766619609043 1101 5 -0.4395007979961285 1101 6 -2.2841168394400526 1101 7 -0.6609376542938463 1101 8 -0.1631698384216378 1101 9 -0.4304307377450627 1101 10 -9.27418377444124 1101 11 -4.195932487210804 1101 12 -1.4675508579328636 1101 13 -0.9098401190505356 1101 14 -0.7336568326034438 1101 15 -2.097114503406857 1101 16 -0.5731968919314809 1101 17 -0.29445988407042734 1101 18 -0.30437449375830283 1101 19 -1.2373707156903535 1101 20 -0.7597524450173552 1101 21 -0.5058272330967739 1101 22 -0.3047664678665427 1101 23 -0.3196225189960771 1101 24 -0.5433049116532516 1101 25 -0.2553456095450386 1102 1 -0.18228764240498663 1102 2 -0.2911060718974916 1102 3 -0.5506456013617225 1102 4 -0.41368363143220677 1102 5 -0.37561319096131385 1102 6 -1.20563660562987 1102 7 -0.19693211668424837 1102 8 0.20619897663245723 1102 9 -0.08077171092867985 1102 10 0.01527425765638668 1102 11 -0.27793012859930855 1102 12 -0.11691249460414355 1102 13 -0.36293451478452 1102 14 -0.32378142881940875 1102 15 -0.009938171560477826 1102 16 -0.006858379907690448 1102 17 -0.2158822293506404 1102 18 -0.5697900708490096 1102 19 -0.31875306712346685 1102 20 -0.4097601361417986 1102 21 -0.3395553774848139 1102 22 -0.4266246435318237 1102 23 -0.47912105768243407 1102 24 -4.0834782183240215 1102 25 -1.8985492192461138 1103 1 -0.3582088491704347 1103 2 -0.5597870969904868 1103 3 -0.310509141508458 1103 4 -0.38721781832466107 1103 5 -0.8391245660409474 1103 6 0.03422446837493709 1103 7 -0.2756965795790869 1103 8 -0.170324379786706 1103 9 -0.2505059841360975 1103 10 -0.22312459969785436 1103 11 -0.4055550284122944 1103 12 -0.4734703770102797 1103 13 -0.47008221613473467 1103 14 -0.5596781796774195 1103 15 -0.29919195811589105 1103 16 -0.20625623025397744 1103 17 -0.5165655561760971 1103 18 -0.6964531753106726 1103 19 -2.197355175674815 1103 20 -0.8213951882390447 1103 21 -0.8899252987737825 1103 22 -0.851337440753305 1103 23 -0.878369911953996 1103 24 0.7580834660509126 1103 25 -0.11306040936510442 1104 1 -0.18214897212142134 1104 2 -0.277886846325476 1104 3 -1.4504173520993575 1104 4 -0.5842824947407586 1104 5 -0.4640433320102453 1104 6 -1.6125195102156056 1104 7 -0.29954002898794485 1104 8 0.17825235963066888 1104 9 -0.16071664769199145 1104 10 0.18553504888675576 1104 11 0.08143138078117573 1104 12 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-1.9684843196744808 2214 25 -1.1251102803820956 2215 1 0.3734848197438829 2215 2 0.13893202116218006 2215 3 0.42828708916955804 2215 4 0.575643273880587 2215 5 0.904449974897453 2215 6 1.342625453506745 2215 7 0.8453960466672363 2215 8 -0.595030910144061 2215 9 0.022096265388526604 2215 10 -0.14386323805484835 2215 11 -0.20738846816419 2215 12 -0.40639335570084256 2215 13 0.038663986126017846 2215 14 -0.09245626540634078 2215 15 -0.08021062931060641 2215 16 0.24043859640071474 2215 17 -0.27096496958763794 2215 18 -0.8653394290733232 2215 19 0.32770814921519786 2215 20 -3.630594312566327 2215 21 -1.7414521127424507 2215 22 -0.8273020489809753 2215 23 -0.7862786820127212 2215 24 -0.5426816950854914 2215 25 -0.32066430779268273 2216 1 -0.4668442968453448 2216 2 -1.1759379333183517 2216 3 -0.24751201552521707 2216 4 -0.40900235148964015 2216 5 -0.4424038081520827 2216 6 -1.8565995999554517 2216 7 -10.13004669575103 2216 8 -11.18413012121329 2216 9 -5.942452130274162 2216 10 -3.43848590728372 2216 11 -2.2162865262411806 2216 12 -2.0247227582597986 2216 13 -1.7345596626277384 2216 14 -1.818730598366237 2216 15 -2.846554506337341 2216 16 -1.8767885656954135 2216 17 -2.2805135302328665 2216 18 -1.8568530417037579 2216 19 -1.1881843827324872 2216 20 -2.174057783076094 2216 21 -5.022942172151872 2216 22 -3.439231700427875 2216 23 -3.2712118483732553 2216 24 -3.625446362425709 2216 25 -3.047407662531846 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35033.0 3 0.0 4 283.0 5 1877.0 6 2415.0 7 1973.0 8 1521.0 9 1258.0 10 1206.0 11 1203.0 12 1166.0 13 1105.0 14 1213.0 15 1262.0 16 1287.0 17 1412.0 18 1551.0 19 2045.0 20 2653.0 21 3446.0 22 4292.0 23 5138.0 24 6015.0 25 6903.0 26 7882.0 27 8654.0 28 9839.0 29 11490.0 30 13942.0 31 16819.0 32 21888.0 33 28218.0 34 37784.0 35 53350.0 36 77167.0 37 123338.0 38 170773.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.68050060435889 18.8794210166743 12.664509493314513 25.775568885652294 2 13.800806208435034 20.247173244801424 43.09089572547508 22.861124821288463 3 18.796835688963768 24.786252979856247 29.262975970619255 27.153935360560734 4 11.488186449877501 16.759527757869453 39.6045556911654 32.147730101087646 5 12.170832265519273 38.184880951845706 37.09233856524756 12.55194821738746 6 31.473299923268748 37.49430510262805 18.73111691924036 12.301278054862843 7 27.248766692662667 31.850810008309704 22.906833642129747 17.993589656897882 8 23.84035411420363 38.448616778955085 20.96137318420309 16.749655922638187 9 26.12647445379083 15.550420287704881 20.491367205120483 37.83173805338381 10 14.274515446796201 29.554485349113634 35.37556901557495 20.795430188515216 11 33.923171217829406 22.529978930079633 25.393899613668175 18.152950238422783 12 23.29332074510333 24.86565061667341 32.488273464310865 19.352755173912392 13 28.85656312565188 22.71018905925935 25.85448311414288 22.578764700945896 14 19.82637442342102 23.281352559126507 27.936843808912958 28.955429208539513 15 22.229898217304257 32.7772649347224 23.140128037596057 21.85270881037728 16 20.848038532492307 28.513774556745958 29.661267142328345 20.97691976843339 17 20.80166885714157 29.574708182285477 28.70341369403986 20.920209266533092 18 20.52230754014638 28.072943828037427 31.23580115928477 20.168947472531425 19 23.450405017571992 27.108376857423323 27.515867247154386 21.925350877850295 20 23.139645012977827 28.00138470115032 28.735995636093342 20.12297464977851 21 22.04236137544095 27.338882012150346 27.337683161847785 23.281073450560914 22 22.302130242246683 29.431360942311873 27.631107681010647 20.6354011344308 23 21.229877443854658 28.809835662669382 28.520154776212898 21.440132117263065 24 23.05129753650141 27.30888367053647 28.996872485212865 20.642946307749252 25 21.86398083029748 28.02999536941989 28.931085279101097 21.174938521181534 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 186.0 1 186.0 2 243.0 3 300.0 4 300.0 5 300.0 6 485.5 7 671.0 8 671.0 9 671.0 10 1056.5 11 1442.0 12 1442.0 13 1442.0 14 2397.0 15 3352.0 16 3352.0 17 3352.0 18 5857.5 19 8363.0 20 8363.0 21 8363.0 22 13235.5 23 18108.0 24 18108.0 25 18108.0 26 26405.5 27 34703.0 28 34703.0 29 34703.0 30 45663.0 31 56623.0 32 56623.0 33 56623.0 34 63812.0 35 71001.0 36 71001.0 37 71001.0 38 75919.5 39 80838.0 40 80838.0 41 80838.0 42 83396.5 43 85955.0 44 85955.0 45 85955.0 46 86798.0 47 87641.0 48 87641.0 49 87641.0 50 82226.5 51 76812.0 52 76812.0 53 76812.0 54 66800.5 55 56789.0 56 56789.0 57 56789.0 58 47936.5 59 39084.0 60 39084.0 61 39084.0 62 31429.0 63 23774.0 64 23774.0 65 23774.0 66 18200.5 67 12627.0 68 12627.0 69 12627.0 70 9188.0 71 5749.0 72 5749.0 73 5749.0 74 4007.0 75 2265.0 76 2265.0 77 2265.0 78 1522.0 79 779.0 80 779.0 81 779.0 82 509.0 83 239.0 84 239.0 85 239.0 86 152.0 87 65.0 88 65.0 89 65.0 90 45.0 91 25.0 92 25.0 93 25.0 94 15.5 95 6.0 96 6.0 97 6.0 98 5.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2106679492538968 2 0.12346400439915432 3 0.12616103362146597 4 0.06817490534176604 5 0.02247524351926353 6 0.02052738908092736 7 0.28678410730580267 8 0.2776441749413021 9 0.017980194815410827 10 0.035960389630821654 11 0.01483366072271393 12 0.016631680204255015 13 0.01453399080912375 14 0.01543300054989429 15 0.01453399080912375 16 0.017980194815410827 17 0.017680524901820645 18 0.01393465098194339 19 0.02187590369208317 20 0.016332010290664833 21 0.01423432089553357 22 0.015882505420279563 23 0.017231020031435375 24 0.014683825765918842 25 0.014683825765918842 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 667401.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.62107157331393 #Duplication Level Percentage of deduplicated Percentage of total 1 93.58865328541778 79.195721281154 2 4.79554169877054 8.116077546489468 3 0.7736972065797175 1.9641326008216597 4 0.2777697294623896 0.9402068863094767 5 0.14597325414126322 0.6176206593238689 6 0.0822369421033714 0.41753868982199194 7 0.05697681867414121 0.3375007615730963 8 0.04859779683933488 0.3289918115717381 9 0.02919633272696385 0.2223562465230026 >10 0.16785967017965867 2.636627158263903 >50 0.01666019647830876 0.9886576003227845 >100 0.014887513314704653 2.4967983249560026 >500 0.0010633938064637768 0.62299977372383 >1k 8.861615053864806E-4 1.1147706591452164 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1858 0.27839334972527763 No Hit CTGTAGGACGTGGAATATGGCAAGA 1631 0.24438081453279212 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1485 0.22250491084070895 No Hit GTCCTACAGTGTGCATTTCTCATTT 1255 0.1880428707778382 No Hit CTTTAGGACGTGAAATATGGCGAGG 1204 0.18040128798128863 No Hit GTATCAACGCAGAGTACTTTTTTTT 993 0.14878611209752457 No Hit CTGTAGGACCTGGAATATGGCGAGA 807 0.1209168101336378 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 1.498349567950902E-4 0.0 13 0.0 0.0 0.0 1.498349567950902E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTGCC 25 0.0060879467 18.967772 9 GGGCTAA 40 0.005125971 14.311637 1 TGTAGAA 220 0.0 12.995882 2 TCCTATA 170 0.0 12.893953 2 GTCTTAC 60 3.9404057E-4 12.721456 1 GTAAGAC 60 3.9807052E-4 12.706127 3 TACTTAG 60 4.0213641E-4 12.690835 7 TAGGACC 345 0.0 12.422424 4 TAGAGTG 110 3.695459E-8 12.114892 5 TAGGACT 95 1.0058502E-6 12.030137 4 CCTACAC 90 7.189159E-6 11.647284 3 CTAGAGT 140 6.493792E-10 11.564685 4 TGTAGGA 1005 0.0 11.474308 2 TAGGACA 275 0.0 11.42863 4 TTAGACA 75 2.0227337E-4 11.428629 4 ACTGTTC 185 0.0 11.310347 8 TTAGGAC 525 0.0 11.253998 3 TGGCGAG 820 0.0 11.217063 18 GGACCTG 535 0.0 11.2082615 6 TAGACAT 85 5.2058662E-5 11.198639 5 >>END_MODULE