FastQCFastQC Report
Thu 26 May 2016
SRR1926934_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926934_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667401
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA19880.29787189410863935No Hit
GTCCTACAGTGGACATTTCTAAATT19410.2908296511392701No Hit
GTATCAACGCAGAGTACTTTTTTTT16380.24542965923035776No Hit
GTCCTAAAGTGTGTATTTCTCATTT14930.2237035904950697No Hit
CTTTAGGACGTGAAATATGGCGAGG14400.21576233778492993No Hit
GTCCTACAGTGTGCATTTCTCATTT13130.19673329827195343No Hit
CTGTAGGACCTGGAATATGGCGAGA10240.15343099575817237No Hit
GGTATCAACGCAGAGTACTTTTTTT10010.14998479175188528No Hit
TATCAACGCAGAGTACTTTTTTTTT8410.12601119866467086No Hit
CTGAAGGACCTGGAATATGGCGAGA8090.12121648004722797No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTTAT508.482362E-515.245621
AATCCCG1101.2732926E-1114.667419
CTTATAC653.295343E-614.6449283
GATATAC1850.013.906481
TAGGACC3900.013.4235094
GTCTAGA801.928498E-613.1017061
GTGCTAG1051.9043E-812.7046841
TTATACT604.020084E-412.6913184
GTAGGAC10000.011.9941973
AAATCCC1353.7471182E-1011.95121518
TGTAGGA10550.011.9201372
GGCGAGG3600.011.86333819
GTCCTAA4750.011.8354171
CTGTAGG10450.011.7624711
GTCTTAC657.813873E-411.7274021
TAGGACT907.283179E-611.6337074
CTGCGTT1552.5465852E-1111.63285411
TATACAC3000.011.4230443
GACGTGG3850.011.3386547
AGGACGT7900.011.2053085