FastQCFastQC Report
Thu 26 May 2016
SRR1926933_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926933_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494656
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12050.24360363565791177No Hit
GGTATCAACGCAGAGTACTTTTTTT7890.1595047871652219No Hit
GTACATGGGAAGCAGTGGTATCAAC6590.1332238970112563No Hit
TATCAACGCAGAGTACTTTTTTTTT6360.12857420106093934No Hit
CCCATGTACTCTGCGTTGATACCAC5680.1148272739034804No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAC250.005861023419.0917151
ACCTAGT250.005986206719.0220078
CCTCTAT350.00209874816.3643261
GGACGTG1250.014.4684666
TAGGACG1351.8189894E-1213.4008074
AGTACAC500.001455880813.3492523
AGGACGT1403.6379788E-1212.9195835
GTACACT751.4396781E-512.6955014
GTCTGGG550.002954788512.1492721
TGTAGGA2050.012.09342
CTAAGGC657.7618414E-411.7356073
GACGTGA657.8751764E-411.7153567
GTGTAGT907.043511E-611.6671581
TCTAGCC600.005706775511.1243763
GACGTGG951.3256118E-511.0216847
CGTGGAA951.3803272E-510.9815569
GTCCTAA1053.256453E-610.9095511
GTCTATA700.001429648710.9095511
GTCTAGA700.001429648710.9095511
CTTAGAT700.001443067110.8973483
Produced by FastQC (version 0.11.5)