FastQCFastQC Report
Thu 26 May 2016
SRR1926933_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926933_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494656
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20030.404927869064562No Hit
GGTATCAACGCAGAGTACTTTTTTT12480.2522965454780696No Hit
TATCAACGCAGAGTACTTTTTTTTT9780.19771315823521798No Hit
GTACATGGGAAGCAGTGGTATCAAC7840.1584939836977617No Hit
CCCATGTACTCTGCGTTGATACCAC6410.12958500452839955No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5930.11988129124078148No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGA602.451281E-514.3164491
CCCTTAT400.0051120614.3164471
TAGGACG2000.013.8097344
ACGTGAA1351.8189894E-1213.3525818
TCAAGAC500.001466624113.3362443
ATACTGC655.3107724E-513.1870156
TCTATAC905.205238E-712.7011843
TTAGGAC1457.2759576E-1212.4821993
AGGACGT1950.012.2101975
TAGGCAC550.003001524112.12385753
GGCGAGG1103.871719E-812.07238119
GACGTGA1353.7471182E-1011.9482557
GTAGGAC2400.011.9073613
TAGGACA802.7843656E-511.9049434
CGTGAAA1459.822543E-1111.7774149
GGACGTG2050.011.6145786
TATGGCG2400.011.46157616
TATACTG752.0205187E-411.4287455
ACTGGGG752.0941012E-411.3848338
ATGGCGA2500.011.38253117
Produced by FastQC (version 0.11.5)