##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926933_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 494656 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.841043068314143 31.0 30.0 33.0 26.0 34.0 2 30.14587915642386 31.0 30.0 34.0 26.0 34.0 3 30.19752919200414 31.0 30.0 34.0 26.0 34.0 4 33.76788515655324 35.0 35.0 37.0 28.0 37.0 5 33.71300863630483 37.0 35.0 37.0 28.0 37.0 6 33.666457093414415 37.0 35.0 37.0 29.0 37.0 7 33.66014563656359 37.0 35.0 37.0 28.0 37.0 8 33.66840390089274 37.0 35.0 37.0 29.0 37.0 9 35.182678467460214 39.0 35.0 39.0 29.0 39.0 10 35.09147367059128 39.0 35.0 39.0 29.0 39.0 11 35.10226703001682 39.0 35.0 39.0 29.0 39.0 12 35.0006772383232 39.0 35.0 39.0 28.0 39.0 13 35.016947130935435 39.0 35.0 39.0 28.0 39.0 14 36.117524097554664 40.0 36.0 41.0 28.0 41.0 15 36.081782086945275 40.0 36.0 41.0 28.0 41.0 16 36.038649081381806 40.0 36.0 41.0 27.0 41.0 17 36.035370034933365 40.0 36.0 41.0 27.0 41.0 18 36.000527639410016 40.0 36.0 41.0 27.0 41.0 19 36.088881970500715 40.0 36.0 41.0 27.0 41.0 20 36.05075648531505 40.0 36.0 41.0 27.0 41.0 21 36.00837349592444 40.0 36.0 41.0 27.0 41.0 22 35.92111689739941 40.0 36.0 41.0 27.0 41.0 23 35.86465543731401 40.0 36.0 41.0 27.0 41.0 24 35.79160467072066 40.0 36.0 41.0 27.0 41.0 25 35.686915755595805 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.18983429572843846 1101 2 -0.19733042145882607 1101 3 -0.025690876453332123 1101 4 0.05588437242604272 1101 5 -0.18701037699729994 1101 6 -0.30027490844666005 1101 7 0.08940101846278736 1101 8 -0.227162948715268 1101 9 -0.27641038880761926 1101 10 -0.18758101940952798 1101 11 0.031184367831393445 1101 12 -0.09417923320754795 1101 13 -0.12522287168582835 1101 14 0.11392753476232542 1101 15 -0.1130440078825714 1101 16 -0.046158043766254764 1101 17 -0.26272048257588665 1101 18 -0.24748188592027276 1101 19 -0.3584487102842857 1101 20 -0.07316819311324707 1101 21 -0.11434925969872722 1101 22 -0.20771916403047186 1101 23 -0.419614562838305 1101 24 -0.16287740559777575 1101 25 -0.24991939215804848 1102 1 -0.40194336657005536 1102 2 -0.2404412990602971 1102 3 -0.11125683074759962 1102 4 -0.1821316691823469 1102 5 -0.15036868242287227 1102 6 -0.24598139690682785 1102 7 -0.107970486137269 1102 8 -0.07624558340959453 1102 9 -0.16788951279367836 1102 10 -0.2653416199492895 1102 11 -0.2867411089944554 1102 12 -0.28519872958268877 1102 13 -0.38127060340844565 1102 14 -0.49180571491071845 1102 15 -0.5079797885313369 1102 16 -0.4355129019847439 1102 17 -0.2276398587523616 1102 18 -0.36738005723739064 1102 19 -0.21778225024845455 1102 20 -0.1249632354851613 1102 21 -0.10791409516262718 1102 22 -0.46011935755788613 1102 23 -0.25460031287042995 1102 24 -0.23226189770170436 1102 25 -0.3459044542606904 1103 1 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0.11439102047760485 2214 9 0.37121830509011033 2214 10 0.2569361708378537 2214 11 0.34151634258283536 2214 12 0.2023016885754103 2214 13 0.3010045111668731 2214 14 0.28766774041326926 2214 15 0.3341471308143298 2214 16 0.3409648687308078 2214 17 0.21698262811747782 2214 18 0.24422968417363933 2214 19 0.22047085248462395 2214 20 0.049830194414809625 2214 21 0.0630874771118215 2214 22 -0.02542394391589653 2214 23 0.103508608950726 2214 24 0.25197354203334754 2214 25 0.25581501183504685 2215 1 0.6423778160358715 2215 2 0.6783971940194959 2215 3 0.4271294270318293 2215 4 0.7801024368965557 2215 5 0.6938503226063872 2215 6 0.7338551189705029 2215 7 0.5778732750591331 2215 8 0.6332184487564376 2215 9 0.32545899236164644 2215 10 0.5392437646763071 2215 11 0.21337831934190632 2215 12 0.36592124281609273 2215 13 0.5053156039456823 2215 14 0.6112742091859431 2215 15 0.572163260075996 2215 16 0.4461037595951609 2215 17 0.4502751878910374 2215 18 0.47658350385020043 2215 19 0.28954932262273303 2215 20 0.473551872199387 2215 21 0.49989320058174513 2215 22 0.2620737447921968 2215 23 0.25143413556970273 2215 24 0.4966789056529066 2215 25 0.4976643298261436 2216 1 -0.7630499188352182 2216 2 -0.4075614797218883 2216 3 -2.1751465037579933 2216 4 -1.9799311689492995 2216 5 -0.7883168078618894 2216 6 -0.37470934759251406 2216 7 -0.3292054446809516 2216 8 -0.4705270399936978 2216 9 -0.5137018663179589 2216 10 -0.4049325711142018 2216 11 -0.48411324430684033 2216 12 -0.5131116680251395 2216 13 -0.5221482432537812 2216 14 -0.5357960253571861 2216 15 -0.7127635132324812 2216 16 -0.4764883564287459 2216 17 -0.6603184688659951 2216 18 -0.6442593776557004 2216 19 -0.713955007898015 2216 20 -0.6452548411544754 2216 21 -0.563797413506478 2216 22 -0.4326475695201566 2216 23 -0.3744412492646845 2216 24 -0.6210043564587338 2216 25 -0.5051059195102496 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12565.0 3 8.0 4 224.0 5 1390.0 6 1928.0 7 1424.0 8 1133.0 9 974.0 10 827.0 11 793.0 12 691.0 13 657.0 14 607.0 15 630.0 16 658.0 17 684.0 18 772.0 19 896.0 20 1228.0 21 1560.0 22 1916.0 23 2402.0 24 2821.0 25 3329.0 26 4012.0 27 4284.0 28 4939.0 29 6298.0 30 7872.0 31 10319.0 32 13296.0 33 17561.0 34 23441.0 35 32894.0 36 48365.0 37 83664.0 38 197594.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.839797297844264 21.05665784297059 12.383936016060746 26.719608843124398 2 13.413503766337323 21.52357553115273 41.063195942636725 23.999724759873228 3 17.69327822844803 26.24127528448841 29.90100531368537 26.164441173378194 4 11.805982339241815 18.993603635657912 38.938979816276365 30.26143420882391 5 12.609527198327624 39.05395187474981 35.211857039460114 13.124663887462445 6 29.280012007236795 39.20890158122327 18.715874702861456 12.795211708678474 7 26.543619275627233 33.34128665944127 22.2969475404002 17.818146524531294 8 24.35376680408494 37.99618679450138 20.47262720962723 17.177419191786445 9 25.441318891946374 17.337953930169412 21.15500094015936 36.06572623772485 10 14.540481090763526 30.904562234900713 33.7250771289823 20.829879545353457 11 32.34894525516455 24.94308785294629 24.081621925429925 18.626344966459236 12 22.173051652683714 26.86788638431214 31.306782573536847 19.6522793894673 13 27.33332120352007 23.42327589866754 26.293796207007393 22.949606690804995 14 20.44592605770475 23.732452451804892 28.35061133393712 27.471010156553238 15 22.45478051847494 30.989420652874898 24.364036284754864 22.191762543895294 16 21.01247551293948 30.39872717826176 27.681851171234552 20.906946137564212 17 20.73983325651165 29.874942383736446 28.088636051203675 21.29658830854823 18 20.409297849801476 29.43281337182504 29.67641897738208 20.481469800991402 19 21.862756593497675 28.56521932502315 29.058291250045485 20.51373283143369 20 21.88000355808575 28.40100435056849 29.381297407449335 20.337694683896427 21 21.91177868042063 27.920104650857162 28.78060319815931 21.3875134705629 22 21.514027987239565 28.887262611845856 28.572497139414622 21.026212261499957 23 21.11961087952387 28.493451988662784 28.892923134150333 21.494013997663014 24 21.118238441897656 28.548901957533868 29.45802410983053 20.874835490737954 25 21.540561031084156 28.24766237048269 28.795552185999497 21.416224412433664 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 327.0 1 327.0 2 325.5 3 324.0 4 324.0 5 324.0 6 530.0 7 736.0 8 736.0 9 736.0 10 1156.0 11 1576.0 12 1576.0 13 1576.0 14 2535.0 15 3494.0 16 3494.0 17 3494.0 18 5864.5 19 8235.0 20 8235.0 21 8235.0 22 12304.5 23 16374.0 24 16374.0 25 16374.0 26 22268.0 27 28162.0 28 28162.0 29 28162.0 30 35004.5 31 41847.0 32 41847.0 33 41847.0 34 47070.0 35 52293.0 36 52293.0 37 52293.0 38 55702.5 39 59112.0 40 59112.0 41 59112.0 42 61026.0 43 62940.0 44 62940.0 45 62940.0 46 63916.0 47 64892.0 48 64892.0 49 64892.0 50 61087.0 51 57282.0 52 57282.0 53 57282.0 54 49188.5 55 41095.0 56 41095.0 57 41095.0 58 34028.0 59 26961.0 60 26961.0 61 26961.0 62 21334.5 63 15708.0 64 15708.0 65 15708.0 66 11818.5 67 7929.0 68 7929.0 69 7929.0 70 5663.0 71 3397.0 72 3397.0 73 3397.0 74 2379.5 75 1362.0 76 1362.0 77 1362.0 78 896.0 79 430.0 80 430.0 81 430.0 82 280.0 83 130.0 84 130.0 85 130.0 86 83.5 87 37.0 88 37.0 89 37.0 90 24.0 91 11.0 92 11.0 93 11.0 94 6.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10754948893776685 2 0.10957109587268728 3 0.016981498253331607 4 0.0 5 0.006064820804761289 6 0.3275003234571096 7 0.02102471212317247 8 0.011927480916030533 9 0.011927480916030533 10 0.006064820804761289 11 0.006873463578729461 12 0.0012129641609522576 13 0.0014151248544443007 14 0.0 15 0.0026280890153965587 16 0.0014151248544443007 17 8.086427739681717E-4 18 8.086427739681717E-4 19 4.0432138698408586E-4 20 0.0016172855479363434 21 0.011927480916030533 22 4.0432138698408586E-4 23 4.0432138698408586E-4 24 6.064820804761288E-4 25 0.006266981498253331 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 494656.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.46093934656656 #Duplication Level Percentage of deduplicated Percentage of total 1 93.39036578190584 81.68009117204932 2 5.3606642439991585 9.37697460603437 3 0.6643316639108653 1.743092140899355 4 0.21354643636839069 0.7470788767556502 5 0.09542585259954883 0.417301735315177 6 0.06255345589523842 0.32825904071829437 7 0.03953760546470779 0.24205972794201008 8 0.02668585939848772 0.18671762642097103 9 0.02212370589642902 0.17414640895759698 >10 0.10276612049532402 1.6599786416715052 >50 0.011810139388741843 0.7123137162299515 >100 0.008799707221272275 1.4667540095888079 >500 9.262849706589151E-4 0.6067930147369023 >1k 4.6314248532945755E-4 0.6584392826801301 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2003 0.404927869064562 No Hit GGTATCAACGCAGAGTACTTTTTTT 1248 0.2522965454780696 No Hit TATCAACGCAGAGTACTTTTTTTTT 978 0.19771315823521798 No Hit GTACATGGGAAGCAGTGGTATCAAC 784 0.1584939836977617 No Hit CCCATGTACTCTGCGTTGATACCAC 641 0.12958500452839955 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 593 0.11988129124078148 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.0216069349204296E-4 0.0 5 0.0 0.0 0.0 2.0216069349204296E-4 0.0 6 0.0 0.0 0.0 2.0216069349204296E-4 0.0 7 0.0 0.0 0.0 2.0216069349204296E-4 0.0 8 0.0 0.0 0.0 2.0216069349204296E-4 0.0 9 0.0 0.0 0.0 2.0216069349204296E-4 0.0 10 0.0 0.0 0.0 2.0216069349204296E-4 0.0 11 0.0 0.0 0.0 2.0216069349204296E-4 0.0 12 0.0 0.0 0.0 2.0216069349204296E-4 0.0 13 0.0 0.0 0.0 2.0216069349204296E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTGA 60 2.451281E-5 14.316449 1 CCCTTAT 40 0.00511206 14.316447 1 TAGGACG 200 0.0 13.809734 4 ACGTGAA 135 1.8189894E-12 13.352581 8 TCAAGAC 50 0.0014666241 13.336244 3 ATACTGC 65 5.3107724E-5 13.187015 6 TCTATAC 90 5.205238E-7 12.701184 3 TTAGGAC 145 7.2759576E-12 12.482199 3 AGGACGT 195 0.0 12.210197 5 TAGGCAC 55 0.0030015241 12.1238575 3 GGCGAGG 110 3.871719E-8 12.072381 19 GACGTGA 135 3.7471182E-10 11.948255 7 GTAGGAC 240 0.0 11.907361 3 TAGGACA 80 2.7843656E-5 11.904943 4 CGTGAAA 145 9.822543E-11 11.777414 9 GGACGTG 205 0.0 11.614578 6 TATGGCG 240 0.0 11.461576 16 TATACTG 75 2.0205187E-4 11.428745 5 ACTGGGG 75 2.0941012E-4 11.384833 8 ATGGCGA 250 0.0 11.382531 17 >>END_MODULE