FastQCFastQC Report
Thu 26 May 2016
SRR1926932_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926932_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616844
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT9900.1604943875599017No Hit
GTCCTACAGTGGACATTTCTAAATT7420.12028973289843137No Hit
GTCCTACAGTGTGCATTTCTCATTT7070.11461568889378837No Hit
GTACATGGGAAGCAGTGGTATCAAC6180.1001874055676962No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGC250.005882991519.0802671
TAGGACC2350.014.1821024
TCACGTC655.3933436E-513.1664548
GTAGGAC4350.012.2715413
CCCCTAC701.0468021E-412.2658871
TGTAGGA4800.012.11502552
CTACACT1353.5470293E-1011.9910044
AGGACCT3700.011.318255
GTATAAG855.0872113E-511.2236861
CTAAGAC600.005722104611.1210853
GTCCTAC4750.011.0464711
CTAGGAC951.3061977E-511.0374663
CTGTAGG4950.010.9856091
TGGGCTA700.001466337410.8772795
AATCCCG700.001511753310.8375919
CAGTCCC700.001511753310.837599
TGCGTTG1752.1827873E-1110.83671212
CCTGTAC1158.4053863E-710.7757083
GGACCTG3450.010.7590476
AGACTGC1252.173565E-710.6597336
Produced by FastQC (version 0.11.5)