FastQCFastQC Report
Thu 26 May 2016
SRR1926932_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926932_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616844
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17050.27640700079760844No Hit
GGTATCAACGCAGAGTACTTTTTTT11120.18027248380465727No Hit
TATCAACGCAGAGTACTTTTTTTTT8590.13925725142823794No Hit
GTACATGGGAAGCAGTGGTATCAAC7520.12191088832832937No Hit
CTGTAGGACGTGGAATATGGCAAGA7400.11996550181245177No Hit
GTCCTACAGTGGACATTTCTAAATT7270.11785799975358438No Hit
GTCCTACAGTGTGCATTTCTCATTT6580.1066720272872882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACAC903.5341145E-813.7487053
GACGTGG1600.013.64267357
CTCCTAC655.287647E-513.1942521
CGTGGAA1700.012.8391229
GGCGAGA2350.012.51945619
ACGTGGA1700.012.28098
TCTGGAC701.0694116E-412.237863
TGGCGAG3500.012.20214418
GCTAGGG550.003020881212.1142443
CTTGAAG657.882785E-411.7148733
TGTAGGA5200.011.544032
TAGGACC2400.011.5003854
GGACGTG3950.011.3246926
AGGACGT4150.011.2376025
ATTATAC855.2072275E-511.1980413
TAGGACG4000.011.1831344
GTGTAGG1451.1496013E-911.1721451
CTGTAGG5300.011.1473451
GTAGGAC5300.011.1346553
TCCTACA5000.011.0529522
Produced by FastQC (version 0.11.5)