##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926932_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 616844 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.827016231008166 31.0 30.0 33.0 26.0 34.0 2 30.145548955651673 31.0 30.0 34.0 26.0 34.0 3 30.194144710818293 31.0 30.0 34.0 26.0 34.0 4 33.76591326169988 35.0 35.0 37.0 28.0 37.0 5 33.73771650530766 37.0 35.0 37.0 28.0 37.0 6 33.670310483687935 37.0 35.0 37.0 29.0 37.0 7 33.64908469564428 37.0 35.0 37.0 28.0 37.0 8 33.66816245274332 37.0 35.0 37.0 29.0 37.0 9 35.20085953660893 39.0 35.0 39.0 29.0 39.0 10 35.092618230865504 39.0 35.0 39.0 29.0 39.0 11 35.11221151539125 39.0 35.0 39.0 29.0 39.0 12 35.014078113753236 39.0 35.0 39.0 28.0 39.0 13 35.02508251681138 39.0 35.0 39.0 28.0 39.0 14 36.11727762610968 40.0 36.0 41.0 28.0 41.0 15 36.098948842819254 40.0 36.0 41.0 28.0 41.0 16 36.07114764835193 40.0 36.0 41.0 28.0 41.0 17 36.0460408142091 40.0 36.0 41.0 28.0 41.0 18 36.015777084643766 40.0 36.0 41.0 27.0 41.0 19 36.0904977595632 40.0 36.0 41.0 27.0 41.0 20 36.056391891629005 40.0 36.0 41.0 27.0 41.0 21 36.00146228219777 40.0 36.0 41.0 27.0 41.0 22 35.91511468053511 40.0 36.0 41.0 27.0 41.0 23 35.85090233511228 40.0 36.0 41.0 27.0 41.0 24 35.768036975313045 40.0 36.0 41.0 27.0 41.0 25 35.650772318446805 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.11625157638976091 1101 2 0.012630263971182387 1101 3 0.2579217821545363 1101 4 0.06575652731621773 1101 5 -0.014353825736307613 1101 6 -0.020391856589085933 1101 7 -0.25655334512300954 1101 8 0.033728405273294015 1101 9 -0.1868894047714562 1101 10 -0.06394884643464138 1101 11 -0.10852022905198311 1101 12 0.09303641400547491 1101 13 0.0026702675145244825 1101 14 -0.06929365218623218 1101 15 -0.040303004547936894 1101 16 0.036645589288532676 1101 17 0.03935339787803116 1101 18 0.13460816980654755 1101 19 0.1269975133279786 1101 20 0.019929209699604655 1101 21 0.05019760399985529 1101 22 -0.15906814850943363 1101 23 -0.13735003153965408 1101 24 -0.14068954275697365 1101 25 -0.21450363725184474 1102 1 -0.5065613546715042 1102 2 -0.3325264346222703 1102 3 -0.2041472665559283 1102 4 -0.17852801513354422 1102 5 -0.2975476301706692 1102 6 -0.3352414307645759 1102 7 -0.19325266235622962 1102 8 -0.07374825278841968 1102 9 -0.022076019647570888 1102 10 -0.2028303634796842 1102 11 -0.045629688870072016 1102 12 -0.20528320769830088 1102 13 -0.15504885719295203 1102 14 -0.1269017971354458 1102 15 -0.20633113550405113 1102 16 -0.17977338342492288 1102 17 -0.04042054117960703 1102 18 -0.1731254305215657 1102 19 -0.06047021810100972 1102 20 -0.17382938520015045 1102 21 -0.23782864512557467 1102 22 -0.1977578635809678 1102 23 -0.29781228635883394 1102 24 -0.40468384901285503 1102 25 -0.27131321046229573 1103 1 -0.47655170808727476 1103 2 -0.529492145400134 1103 3 -0.2507841165076954 1103 4 -0.5316222301062083 1103 5 -0.4972377701923065 1103 6 -0.5681333597891012 1103 7 -0.6695717596698074 1103 8 -0.6185347124920071 1103 9 -0.38838313181103956 1103 10 -0.5685236021010951 1103 11 -0.3686031753794694 1103 12 -0.5457900918515932 1103 13 -0.4403948187820035 1103 14 -0.5426052669825623 1103 15 -0.49846478454377774 1103 16 -0.549605883644162 1103 17 -0.4401399132746704 1103 18 -0.5288388392736465 1103 19 -0.5282126250699335 1103 20 -0.5384936548367989 1103 21 -0.5697880710076504 1103 22 -0.5231459193557626 1103 23 -0.5082656758177464 1103 24 -0.4906144520705311 1103 25 -0.525714099117323 1104 1 -0.6722075142693953 1104 2 -0.5708574634571839 1104 3 -0.28728011841723244 1104 4 -0.4199223454739922 1104 5 -0.5422948573546549 1104 6 -0.40882238393677284 1104 7 -0.47658134713051936 1104 8 -0.4061704685488152 1104 9 -0.4996851964965572 1104 10 -0.480088227951434 1104 11 -0.4499653108769479 1104 12 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0.10738992349109822 1105 23 -0.07155546977708838 1105 24 0.09209550256167631 1105 25 0.315550028323905 1106 1 0.15818577665021039 1106 2 0.10561163970364973 1106 3 0.3949118541882761 1106 4 0.3475698740858917 1106 5 0.24310614515161433 1106 6 0.3440970024412593 1106 7 0.2468710567730099 1106 8 0.29327847432117693 1106 9 0.11302101959311273 1106 10 0.33501730143980524 1106 11 0.11813878636249342 1106 12 0.11989977383827011 1106 13 0.09190066351347781 1106 14 0.0946250952477996 1106 15 0.23602849994951214 1106 16 0.2833611493228716 1106 17 0.13821784008307958 1106 18 0.18190597147115994 1106 19 0.13313251127520687 1106 20 0.125507159164016 1106 21 0.15424530302572492 1106 22 0.06473564352580041 1106 23 0.2129186953666249 1106 24 0.3081543716982935 1106 25 0.44859400019077356 1107 1 0.286431220933391 1107 2 0.30441022057770084 1107 3 0.15972801287235683 1107 4 0.3395268165145353 1107 5 0.1642277384430173 1107 6 0.17940582359914004 1107 7 0.10318455459594134 1107 8 0.17239071428247854 1107 9 -0.202129762538803 1107 10 -0.1279725389085229 1107 11 -0.013876211218544654 1107 12 -0.06684429969825345 1107 13 -0.016106109587632034 1107 14 -0.12798012971591533 1107 15 -0.02413115171957969 1107 16 0.18240968988084205 1107 17 -0.034838924954819106 1107 18 -0.10562829719518163 1107 19 -0.15087410056159456 1107 20 -0.12392841574325786 1107 21 -0.0589864337909205 1107 22 0.060441981213273266 1107 23 0.0902368698546141 1107 24 0.21835030783763187 1107 25 0.019409191288985994 1108 1 0.7848545624099046 1108 2 0.7990760036225595 1108 3 0.9130654177963855 1108 4 1.0810674371289153 1108 5 0.9944835556199294 1108 6 0.9719842666921608 1108 7 0.9477169280734259 1108 8 0.8523658515903776 1108 9 1.0778440753252596 1108 10 0.9334933203703741 1108 11 1.0623448328412621 1108 12 0.9217292574859357 1108 13 0.9042726349763583 1108 14 0.8004508351644475 1108 15 0.978788265436549 1108 16 0.790371815424443 1108 17 0.8918291277852788 1108 18 1.0466990067187396 1108 19 0.9244459957705047 1108 20 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2214 8 0.18390059953234328 2214 9 0.05821395451207678 2214 10 0.015298968022840143 2214 11 0.22103950056457222 2214 12 0.08536885574570618 2214 13 0.05301679243782331 2214 14 0.1916340604074449 2214 15 0.26578831395589475 2214 16 0.25289465995226834 2214 17 0.34096467467922764 2214 18 0.33474019964785384 2214 19 0.5197905640291438 2214 20 0.3543236242818537 2214 21 0.320021307842822 2214 22 0.4084913931034677 2214 23 0.4262635696704038 2214 24 0.31305301187090606 2214 25 0.4516360494656979 2215 1 0.21900261938194632 2215 2 0.15235980879224442 2215 3 0.1111267858287377 2215 4 0.10858633988959099 2215 5 -0.007296820676884863 2215 6 0.07946624312965156 2215 7 -0.19368011782071903 2215 8 -0.095827396167671 2215 9 -0.2639442137550958 2215 10 -0.34088244892802777 2215 11 -0.24884735987897244 2215 12 -0.06455525104228599 2215 13 -0.06196615524695659 2215 14 -0.06375700728324318 2215 15 0.01193403649334357 2215 16 -0.3214182320522596 2215 17 -0.17789128719626035 2215 18 -0.2571738612256951 2215 19 -0.13907607210359174 2215 20 -0.3233554439539077 2215 21 -0.2859793659223513 2215 22 -0.1529423640458205 2215 23 -0.27900958911484963 2215 24 -0.22676726694674443 2215 25 -0.12476437901867143 2216 1 -0.30050298306897005 2216 2 -0.22318188168064523 2216 3 -2.0612176258617154 2216 4 -1.6707959194270998 2216 5 -0.6916746857283371 2216 6 -0.2756596720259168 2216 7 -0.1958497269683832 2216 8 -0.30328322098928595 2216 9 -0.18734258474497523 2216 10 -0.5084273359204374 2216 11 -0.2176594656526376 2216 12 -0.1911224282536068 2216 13 -0.1263521065057276 2216 14 -0.4177686962186158 2216 15 -0.3484203899459004 2216 16 -0.37226737872960314 2216 17 -0.4547916882797338 2216 18 -0.4699606985505582 2216 19 -0.43819076527378087 2216 20 -0.5591405178781628 2216 21 -0.4337557538173371 2216 22 -0.4159633407305918 2216 23 -0.5552741030028159 2216 24 -0.44622712348300553 2216 25 -0.6334969134752129 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15661.0 3 25.0 4 242.0 5 1602.0 6 2263.0 7 1768.0 8 1335.0 9 1145.0 10 1019.0 11 888.0 12 920.0 13 813.0 14 792.0 15 798.0 16 811.0 17 864.0 18 998.0 19 1137.0 20 1424.0 21 1905.0 22 2438.0 23 3009.0 24 3530.0 25 4389.0 26 4944.0 27 5614.0 28 6349.0 29 7934.0 30 10057.0 31 12805.0 32 16803.0 33 21675.0 34 29681.0 35 41452.0 36 61113.0 37 104322.0 38 244319.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.592239564652175 19.628792292798355 10.554717918172656 28.224250224376817 2 13.915062952997042 20.881277041874537 40.48207162262607 24.721588382502347 3 18.431225029834483 24.73570539096145 29.127049499299538 27.70602007990453 4 11.816277697440519 17.672053225775073 38.008313285044515 32.50335579173989 5 12.717934417830984 38.71734428855376 35.36138933292695 13.203331960688311 6 31.27910513551628 38.43154732375076 17.70112736399169 12.588220176741267 7 28.23717122384749 32.06371134990166 21.402129657104776 18.296987769146078 8 24.907340452094285 38.33186872338977 19.6347446552546 17.12604616926135 9 25.879249516842417 16.331861161912915 19.95051688133131 37.83837243991336 10 14.188557134079415 30.279773224322774 34.07179716643889 21.45987247515892 11 33.98188424006705 23.70077706136888 23.824153589997586 18.493185108566482 12 22.683118205310272 25.8181187402933 31.404962697092607 20.093800357303813 13 28.384137889666867 22.63630335856404 25.684414622244628 23.295144129524463 14 20.311132150106022 22.95102165215192 27.739428445441632 28.99841775230042 15 22.55213804910315 31.349461110462627 23.472173585982386 22.626227254451837 16 21.491402984881056 29.59590948735657 27.616634405569013 21.296053122193364 17 21.064227144066066 29.507394113889777 27.729142957191634 21.699235784852522 18 20.944133091023456 28.772082270795877 29.501605762262884 20.782178875917783 19 22.577742760706958 27.9512419712017 27.95464640864273 21.51636885944861 20 22.513779910511637 28.221580961027172 28.549380714609946 20.71525841385124 21 22.183957805373556 27.449692830772378 27.91389209110174 22.45245727275233 22 22.062790592110808 28.53655057032248 27.89651840659875 21.50414043096796 23 21.583287805811203 28.31838895796823 28.048141909691772 22.050181326528794 24 22.018730860202677 27.73882495221637 28.801980406683708 21.440463780897247 25 21.97031476722789 28.000907904443057 28.253662016358494 21.77511531197056 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 296.0 1 296.0 2 286.0 3 276.0 4 276.0 5 276.0 6 439.0 7 602.0 8 602.0 9 602.0 10 996.5 11 1391.0 12 1391.0 13 1391.0 14 2302.0 15 3213.0 16 3213.0 17 3213.0 18 5618.0 19 8023.0 20 8023.0 21 8023.0 22 12192.5 23 16362.0 24 16362.0 25 16362.0 26 23082.5 27 29803.0 28 29803.0 29 29803.0 30 38351.5 31 46900.0 32 46900.0 33 46900.0 34 53827.0 35 60754.0 36 60754.0 37 60754.0 38 66131.5 39 71509.0 40 71509.0 41 71509.0 42 75108.0 43 78707.0 44 78707.0 45 78707.0 46 80883.0 47 83059.0 48 83059.0 49 83059.0 50 79150.0 51 75241.0 52 75241.0 53 75241.0 54 65640.5 55 56040.0 56 56040.0 57 56040.0 58 47615.5 59 39191.0 60 39191.0 61 39191.0 62 31484.0 63 23777.0 64 23777.0 65 23777.0 66 18251.5 67 12726.0 68 12726.0 69 12726.0 70 9159.5 71 5593.0 72 5593.0 73 5593.0 74 3908.5 75 2224.0 76 2224.0 77 2224.0 78 1516.0 79 808.0 80 808.0 81 808.0 82 527.0 83 246.0 84 246.0 85 246.0 86 161.5 87 77.0 88 77.0 89 77.0 90 51.0 91 25.0 92 25.0 93 25.0 94 13.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1123460712919312 2 0.10861741380316581 3 0.017508478642898367 4 0.0 5 0.0048634662896939905 6 0.33185051650012 7 0.02026444287372496 8 0.01134808800928598 9 0.011023856923306378 10 0.003728657488765393 11 0.00534981291866339 12 3.242310859795994E-4 13 4.8634662896939905E-4 14 0.0 15 0.003242310859795994 16 3.242310859795994E-4 17 3.242310859795994E-4 18 4.8634662896939905E-4 19 3.242310859795994E-4 20 6.484621719591988E-4 21 0.013455590068153373 22 0.0 23 1.621155429897997E-4 24 1.621155429897997E-4 25 0.006322506176602188 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 616844.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.3582113850815 #Duplication Level Percentage of deduplicated Percentage of total 1 93.11252625852737 80.41031225232858 2 5.3990068435504694 9.324971485296663 3 0.7505461452122298 1.9444746798748747 4 0.26332228661593443 0.9096016675992755 5 0.12869670721961246 0.5557008723317619 6 0.0784123933507325 0.40629324241156306 7 0.046586747318253796 0.2816203720857198 8 0.03668675718256213 0.25345621854438866 9 0.02768604084662675 0.21518252710640945 >10 0.1343328868802691 2.1370495015294884 >50 0.010534195217725454 0.6379357437681656 >100 0.009405433934465116 1.4129665315616098 >500 0.001881086786449307 1.0528208886797543 >1k 3.762173572898614E-4 0.457614016881788 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1705 0.27640700079760844 No Hit GGTATCAACGCAGAGTACTTTTTTT 1112 0.18027248380465727 No Hit TATCAACGCAGAGTACTTTTTTTTT 859 0.13925725142823794 No Hit GTACATGGGAAGCAGTGGTATCAAC 752 0.12191088832832937 No Hit CTGTAGGACGTGGAATATGGCAAGA 740 0.11996550181245177 No Hit GTCCTACAGTGGACATTTCTAAATT 727 0.11785799975358438 No Hit GTCCTACAGTGTGCATTTCTCATTT 658 0.1066720272872882 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTACAC 90 3.5341145E-8 13.748705 3 GACGTGG 160 0.0 13.6426735 7 CTCCTAC 65 5.287647E-5 13.194252 1 CGTGGAA 170 0.0 12.839122 9 GGCGAGA 235 0.0 12.519456 19 ACGTGGA 170 0.0 12.2809 8 TCTGGAC 70 1.0694116E-4 12.23786 3 TGGCGAG 350 0.0 12.202144 18 GCTAGGG 55 0.0030208812 12.114244 3 CTTGAAG 65 7.882785E-4 11.714873 3 TGTAGGA 520 0.0 11.54403 2 TAGGACC 240 0.0 11.500385 4 GGACGTG 395 0.0 11.324692 6 AGGACGT 415 0.0 11.237602 5 ATTATAC 85 5.2072275E-5 11.198041 3 TAGGACG 400 0.0 11.183134 4 GTGTAGG 145 1.1496013E-9 11.172145 1 CTGTAGG 530 0.0 11.147345 1 GTAGGAC 530 0.0 11.134655 3 TCCTACA 500 0.0 11.052952 2 >>END_MODULE