FastQCFastQC Report
Thu 26 May 2016
SRR1926931_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926931_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613815
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT10640.17334213077230112No Hit
GTCCTACAGTGGACATTTCTAAATT9300.15151144888932333No Hit
GTCCTACAGTGTGCATTTCTCATTT7930.12919202039702518No Hit
CTGTAGGACGTGGAATATGGCAAGA7090.11550711533605404No Hit
GTCCTAAAGTGTGTATTTCTCATTT6350.1034513656394842No Hit
GGTATCAACGCAGAGTACTTTTTTT6300.10263678795728354No Hit
GTACATGGGAAGCAGTGGTATCAAC6210.10117054812932234No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCAC350.002194826316.25596813
GCCCTAC456.549523E-414.8378181
AGGCGTG456.668505E-414.8026397
GGCGTGC456.7058066E-414.7917578
TAAGTTG400.00533864514.22397118
TGCGCCA400.00533864514.22397112
CCAACGA551.9876969E-413.79294219
TAGGACC2150.012.841814
TAGACAG1051.9361323E-812.6890145
TCCAACG905.507882E-712.6435318
AGGACGT3350.012.2155445
TTAATAC550.002982237412.1350613
CTACACT951.00951E-612.0260884
GGACGTG3350.011.9314616
GTATTAA802.7434404E-511.9232451
AGGACCT2950.011.6136735
TTAGGAC2300.011.6074493
GTAGGAC4700.011.5633493
GACGTGG1900.011.5193477
TGTAGGA4850.011.402312
Produced by FastQC (version 0.11.5)