##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926931_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 613815 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.509721984637064 31.0 29.0 31.0 22.0 34.0 2 28.535610892532766 31.0 30.0 31.0 22.0 34.0 3 28.257285990078444 31.0 28.0 33.0 16.0 34.0 4 32.09904287122342 35.0 32.0 37.0 20.0 37.0 5 32.0318336958204 35.0 32.0 37.0 19.0 37.0 6 31.567861652126457 35.0 32.0 37.0 17.0 37.0 7 31.571147658496454 35.0 32.0 37.0 17.0 37.0 8 31.423179622524703 35.0 32.0 37.0 17.0 37.0 9 33.579356972377674 37.0 34.0 39.0 19.0 39.0 10 33.72751236121633 38.0 34.0 39.0 22.0 39.0 11 33.87000317685296 38.0 34.0 39.0 23.0 39.0 12 33.766947695967026 38.0 34.0 39.0 22.0 39.0 13 33.857540138315294 38.0 35.0 39.0 23.0 39.0 14 34.900412990884874 39.0 35.0 41.0 23.0 41.0 15 34.498620919984035 39.0 34.0 41.0 17.0 41.0 16 34.57179769148685 39.0 34.0 41.0 18.0 41.0 17 34.80228407582089 39.0 35.0 41.0 22.0 41.0 18 34.857331606428644 39.0 35.0 41.0 22.0 41.0 19 34.27303829329684 39.0 34.0 41.0 17.0 41.0 20 34.55327745330433 39.0 34.0 41.0 18.0 41.0 21 34.523011004944486 39.0 34.0 41.0 18.0 41.0 22 34.54359701212906 39.0 35.0 41.0 18.0 41.0 23 34.54737665257447 39.0 35.0 41.0 18.0 41.0 24 33.278175020160795 39.0 33.0 41.0 2.0 41.0 25 33.85331899676613 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.3405408216995518 1101 2 -0.3067825965239237 1101 3 -1.2128815621685973 1101 4 -0.6918980141236837 1101 5 -0.44228390423079844 1101 6 -2.4371013085904174 1101 7 -0.6419572585061815 1101 8 -0.12834524983671258 1101 9 -0.16166480188455523 1101 10 -9.296717567989148 1101 11 -4.061867156052227 1101 12 -1.6846912909520242 1101 13 -1.0333861357122345 1101 14 -0.6602034099133505 1101 15 -1.680004086639208 1101 16 -0.2745802400533961 1101 17 -0.30658922153374135 1101 18 -0.15372754502650565 1101 19 -0.9909931231050706 1101 20 -0.3797816889366956 1101 21 -0.1969529879727574 1101 22 -0.09523523606268469 1101 23 -0.15319739163129498 1101 24 -0.28057012716189433 1101 25 -0.11484318127919124 1102 1 -0.3945857971963669 1102 2 -0.5141161577938895 1102 3 -0.8041695840984993 1102 4 -0.4977523866081306 1102 5 -0.3166596277796536 1102 6 -0.9402618299654755 1102 7 -0.26563987473886996 1102 8 0.34189642297824463 1102 9 0.2520437871276968 1102 10 0.021630731183684304 1102 11 -0.22818531960275834 1102 12 -0.39516257993368953 1102 13 -0.30596955752097443 1102 14 -0.4029865162098005 1102 15 0.11225806448278064 1102 16 -0.003960672293231937 1102 17 -0.1800208075528289 1102 18 -0.426858014755922 1102 19 -0.06164148286105586 1102 20 0.0012395871485537668 1102 21 -0.17747520279703366 1102 22 -0.25742774654477074 1102 23 -0.5637520574342005 1102 24 -4.281250338063259 1102 25 -2.1463128327160916 1103 1 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-3.3005427046011704 2216 11 -2.3710291599133555 2216 12 -1.8828392573143837 2216 13 -1.6940287848402065 2216 14 -1.8566204119748022 2216 15 -3.1406630964883604 2216 16 -2.097308553917472 2216 17 -2.4414277412156906 2216 18 -2.0131333236332267 2216 19 -1.4157992485590825 2216 20 -2.2651617484897173 2216 21 -4.953377843180373 2216 22 -3.4183660236678044 2216 23 -3.431807384759793 2216 24 -3.3896241502632307 2216 25 -3.0614025216149123 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31436.0 3 2.0 4 244.0 5 1520.0 6 1951.0 7 1550.0 8 1327.0 9 1181.0 10 1106.0 11 1021.0 12 1099.0 13 1034.0 14 1029.0 15 1047.0 16 1144.0 17 1259.0 18 1422.0 19 1772.0 20 2309.0 21 3011.0 22 3671.0 23 4486.0 24 5164.0 25 6011.0 26 6926.0 27 7578.0 28 8368.0 29 9790.0 30 11936.0 31 14470.0 32 18713.0 33 25163.0 34 33991.0 35 47918.0 36 70956.0 37 116065.0 38 166145.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.75739886516716 19.27778911703474 12.781320161652449 25.18349185614565 2 13.817241576195563 20.365745234463574 43.35190335973377 22.465109829607094 3 18.750244704592617 25.07455333255028 29.503380186106 26.671821776751102 4 10.98543513630717 17.362250038311426 40.05516845616358 31.59714636921783 5 11.746388678415173 39.31350263567408 36.6691923512494 12.270916334661354 6 29.96072935847089 38.592774853753525 19.555149994296794 11.891345793478793 7 26.7412660990836 31.79478130733626 23.74764936632961 17.71630322725052 8 23.546622754138856 38.38964526911126 21.494920980321044 16.56881099642884 9 25.686687621291593 16.107523622836304 20.96489041187407 37.24089834399803 10 13.123001554753733 30.668413727465033 36.00705343202923 20.201531285752004 11 33.46515197434669 23.59244087713981 24.858323475449797 18.0840836730637 12 21.902078804923434 25.981313893351672 33.176638130216155 18.93996917150874 13 28.051397445443982 22.91311935111214 26.472682479343423 22.562800724100455 14 19.422144082696096 23.36027843452275 29.095821038035446 28.121756444745706 15 22.162912560613172 32.20873090359247 23.9979274206163 21.63042911517806 16 20.509010087974435 30.11547968799138 29.021461591230906 20.35404863280327 17 20.36808916263382 29.892131462742988 28.92766942041029 20.8121099542129 18 19.916445886980352 29.446959106062238 30.79265610182913 19.84393890512828 19 22.12441582312721 28.319623780316906 28.699135059737586 20.856825336818297 20 21.81569916931991 28.854319764580893 29.569147844109793 19.760833221989397 21 21.77593331247719 28.229769539600603 28.281909901454718 21.71238724646749 22 21.094902047686475 29.538370291192845 28.950477174332796 20.416250486787884 23 20.922070176925107 28.99091747514282 28.87718223383835 21.20983011409372 24 21.527732516457014 28.429088183536464 29.633546242586196 20.409633057420322 25 21.27891156462585 28.555623446983585 29.286243838852904 20.87922114953766 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 153.0 1 153.0 2 232.0 3 311.0 4 311.0 5 311.0 6 513.5 7 716.0 8 716.0 9 716.0 10 1139.5 11 1563.0 12 1563.0 13 1563.0 14 2670.0 15 3777.0 16 3777.0 17 3777.0 18 6598.5 19 9420.0 20 9420.0 21 9420.0 22 14363.0 23 19306.0 24 19306.0 25 19306.0 26 27096.5 27 34887.0 28 34887.0 29 34887.0 30 44717.5 31 54548.0 32 54548.0 33 54548.0 34 61783.5 35 69019.0 36 69019.0 37 69019.0 38 73520.0 39 78021.0 40 78021.0 41 78021.0 42 80026.5 43 82032.0 44 82032.0 45 82032.0 46 81655.5 47 81279.0 48 81279.0 49 81279.0 50 75324.0 51 69369.0 52 69369.0 53 69369.0 54 58699.0 55 48029.0 56 48029.0 57 48029.0 58 39391.5 59 30754.0 60 30754.0 61 30754.0 62 23879.5 63 17005.0 64 17005.0 65 17005.0 66 12616.5 67 8228.0 68 8228.0 69 8228.0 70 5816.0 71 3404.0 72 3404.0 73 3404.0 74 2368.0 75 1332.0 76 1332.0 77 1332.0 78 902.5 79 473.0 80 473.0 81 473.0 82 303.5 83 134.0 84 134.0 85 134.0 86 86.0 87 38.0 88 38.0 89 38.0 90 24.5 91 11.0 92 11.0 93 11.0 94 6.5 95 2.0 96 2.0 97 2.0 98 3.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22645259565178436 2 0.13359073988090875 3 0.13538281078175018 4 0.06858744084129584 5 0.019549864372815914 6 0.020364442055016578 7 0.2844505266244715 8 0.275164341047384 9 0.017594877935534322 10 0.034701009261748246 11 0.015151144888932334 12 0.015802807034692865 13 0.014336567206731671 14 0.015965722571132997 15 0.014173651670291536 16 0.018572371154175118 17 0.017106131326213924 18 0.013196158451650742 19 0.02134193527365737 20 0.016454469180453395 21 0.014173651670291536 22 0.015965722571132997 23 0.017757793471974457 24 0.015476975961812599 25 0.014662398279611936 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 613815.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.48195606876423 #Duplication Level Percentage of deduplicated Percentage of total 1 94.70673551699629 83.79837211430939 2 4.1512936472356525 7.346291642464902 3 0.5714035655172055 1.5167671554488589 4 0.18495856637805283 0.6546198297921794 5 0.09528557827610837 0.4215527175506708 6 0.06053490756810936 0.32137482192408934 7 0.040497482443949843 0.25083075237507846 8 0.026836039312503175 0.18996042012068287 9 0.018415720283889114 0.14665130578203542 >10 0.12173688942753315 2.0346478870098195 >50 0.011243287947620369 0.6559323554993297 >100 0.009584292131552423 1.7017817712406573 >500 0.0012901931712268756 0.7876958269273405 >1k 1.8431331017526795E-4 0.17352139955500837 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1064 0.17334213077230112 No Hit GTCCTACAGTGGACATTTCTAAATT 930 0.15151144888932333 No Hit GTCCTACAGTGTGCATTTCTCATTT 793 0.12919202039702518 No Hit CTGTAGGACGTGGAATATGGCAAGA 709 0.11550711533605404 No Hit GTCCTAAAGTGTGTATTTCTCATTT 635 0.1034513656394842 No Hit GGTATCAACGCAGAGTACTTTTTTT 630 0.10263678795728354 No Hit GTACATGGGAAGCAGTGGTATCAAC 621 0.10117054812932234 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCCAC 35 0.0021948263 16.255968 13 GCCCTAC 45 6.549523E-4 14.837818 1 AGGCGTG 45 6.668505E-4 14.802639 7 GGCGTGC 45 6.7058066E-4 14.791757 8 TAAGTTG 40 0.005338645 14.223971 18 TGCGCCA 40 0.005338645 14.223971 12 CCAACGA 55 1.9876969E-4 13.792942 19 TAGGACC 215 0.0 12.84181 4 TAGACAG 105 1.9361323E-8 12.689014 5 TCCAACG 90 5.507882E-7 12.64353 18 AGGACGT 335 0.0 12.215544 5 TTAATAC 55 0.0029822374 12.135061 3 CTACACT 95 1.00951E-6 12.026088 4 GGACGTG 335 0.0 11.931461 6 GTATTAA 80 2.7434404E-5 11.923245 1 AGGACCT 295 0.0 11.613673 5 TTAGGAC 230 0.0 11.607449 3 GTAGGAC 470 0.0 11.563349 3 GACGTGG 190 0.0 11.519347 7 TGTAGGA 485 0.0 11.40231 2 >>END_MODULE