FastQCFastQC Report
Thu 26 May 2016
SRR1926931_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926931_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613815
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17210.2803776382134682No Hit
GGTATCAACGCAGAGTACTTTTTTT9760.1590055635655694No Hit
GTCCTACAGTGGACATTTCTAAATT9120.14857896923340094No Hit
TATCAACGCAGAGTACTTTTTTTTT8500.13847820597411273No Hit
CTGTAGGACGTGGAATATGGCAAGA8420.13717488168259168No Hit
GTCCTACAGTGTGCATTTCTCATTT7800.12707411842330343No Hit
GTACATGGGAAGCAGTGGTATCAAC7020.1143667065809731No Hit
CACACACACACACACACACACACAC6810.11094548031573032No Hit
GTCCTAAAGTGTGTATTTCTCATTT6390.10410302778524473No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAA456.5837643E-414.8276231
CCTACAC456.628856E-414.8142853
TAAGACT759.3804556E-713.9677544
CTACACT1051.3133103E-913.6049564
CTTAGAC707.063005E-613.6049563
ACGTGAA2200.012.9389368
TAGGACC2750.012.8133954
GTTCTAG751.4249106E-512.7093911
AGGACGT4250.012.3244895
GCCTTAG701.0551167E-412.2554851
TAGACTG1404.9112714E-1112.244465
GTCCTAC4000.012.1533541
GTCTTAG1103.6226993E-812.1316921
TAGGCCT550.003008670612.1207784
GGACGTG4300.011.9597056
CTCCTAA802.762823E-511.9150531
GGCAAGA3750.011.89135119
TCCTACA4450.011.7811772
GACGTGA2100.011.7515637
GTCTAAT657.788048E-411.7317451
Produced by FastQC (version 0.11.5)