FastQCFastQC Report
Thu 26 May 2016
SRR1926930_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926930_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777817
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT18780.24144496713237174No Hit
GTCCTACAGTGTGCATTTCTCATTT15690.2017183990578761No Hit
GTCCTAAAGTGTGTATTTCTCATTT14630.18809051486403616No Hit
CTGTAGGACGTGGAATATGGCAAGA14570.1873191251926867No Hit
CTTTAGGACGTGAAATATGGCGAGG11190.1438641737066688No Hit
CTGTAGGACCTGGAATATGGCGAGA11100.14270708919964464No Hit
GTATCAACGCAGAGTACTTTTTTTT9710.12483656181338285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG453.59518E-516.85579718
TCGAACT453.59518E-516.85579719
GTCGTCA400.005123302314.3131881
TAGGACC4200.013.3775814
GGCGAGG3650.012.988219
TCTAGAC701.0536874E-412.2581353
ACTACTC550.003040229412.1045768
GTCGAGG550.003111440512.06721919
TGGCGAG8400.011.96460718
CTCGAAC658.14847E-411.66939818
TGTCGAG658.14847E-411.66939818
CTAAGAC907.1556624E-611.6527953
GTAGGAC9550.011.4808593
ATGGCGA9300.011.41844317
GTCCTAC10700.011.4148791
AGGACGT7700.011.373755
ACGTGAA4100.011.3664928
CTGTAGG9750.011.3526821
TATGGCG9500.011.27785916
GACGTGG3600.011.1058897
Produced by FastQC (version 0.11.5)