FastQCFastQC Report
Thu 26 May 2016
SRR1926930_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926930_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777817
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT18870.24260205163939588No Hit
CTGTAGGACGTGGAATATGGCAAGA18420.23681662910427517No Hit
GTCCTACAGTGTGCATTTCTCATTT16480.2118750297306436No Hit
GTATCAACGCAGAGTACTTTTTTTT15530.19966135993427758No Hit
GTCCTAAAGTGTGTATTTCTCATTT14400.18513352112386333No Hit
CTTTAGGACGTGAAATATGGCGAGG14190.18243365727414032No Hit
CTGTAGGACCTGGAATATGGCGAGA12300.15813488262663325No Hit
GGTATCAACGCAGAGTACTTTTTTT9630.12380804225158361No Hit
TATCAACGCAGAGTACTTTTTTTTT8330.10709459937234594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4200.014.7423464
AGGACCT6600.012.8454285
GGACCTG6200.012.7523126
ACTGGAC603.9931823E-412.7019193
GGCGAGG3300.012.36295319
GTCCTAC9950.012.2646181
TGTAGGA10650.012.2641682
GTAGGAC10000.012.1938413
CTAGGAC1251.3442332E-912.1938413
TAGACAA951.0040621E-612.03261855
GATATAC1850.011.8528631
ACTGTTC1850.011.7934278
CTGTAGG10950.011.754031
TCCTACA11000.011.70060352
TACACTG3750.011.6850095
TCAGTAC752.0184743E-411.43172553
TGGCGAG9550.011.42515718
TAGGACA3150.011.1890624
GGCGAGA6200.011.17117519
CTACTAA600.005717845611.1227951
Produced by FastQC (version 0.11.5)