##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926930_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 777817 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.79990409055086 31.0 30.0 33.0 26.0 34.0 2 30.17469276192215 31.0 30.0 34.0 26.0 34.0 3 30.25346964645926 31.0 30.0 34.0 26.0 34.0 4 33.85064353183332 35.0 35.0 37.0 29.0 37.0 5 33.80900520302333 37.0 35.0 37.0 30.0 37.0 6 33.764566729706345 37.0 35.0 37.0 29.0 37.0 7 33.760978482085115 37.0 35.0 37.0 29.0 37.0 8 33.78814425501114 37.0 35.0 37.0 29.0 37.0 9 35.34272328838274 39.0 35.0 39.0 30.0 39.0 10 35.23016082188998 39.0 35.0 39.0 29.0 39.0 11 35.268291898994235 39.0 35.0 39.0 30.0 39.0 12 35.164390852861274 39.0 35.0 39.0 29.0 39.0 13 35.178246297008165 39.0 35.0 39.0 29.0 39.0 14 36.26313773034017 40.0 36.0 41.0 29.0 41.0 15 36.25874466616183 40.0 36.0 41.0 29.0 41.0 16 36.24116855250014 40.0 36.0 41.0 29.0 41.0 17 36.21284698071654 40.0 36.0 41.0 29.0 41.0 18 36.19171990326773 40.0 36.0 41.0 29.0 41.0 19 36.26474479215548 40.0 37.0 41.0 29.0 41.0 20 36.22740310381491 40.0 37.0 41.0 29.0 41.0 21 36.16984714913662 40.0 37.0 41.0 29.0 41.0 22 36.08767229309722 40.0 37.0 41.0 28.0 41.0 23 36.0214664889042 40.0 36.0 41.0 28.0 41.0 24 35.949926525133804 40.0 36.0 41.0 28.0 41.0 25 35.83873713225604 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.13082322228768817 1101 2 -0.0483852913631857 1101 3 0.16730152755765104 1101 4 0.11630057907612468 1101 5 0.08877570728436979 1101 6 0.05354898008884135 1101 7 0.03090691387728839 1101 8 0.04522583680133607 1101 9 0.10690318846586422 1101 10 0.029359396801687865 1101 11 0.1574516782663764 1101 12 0.14051304355675853 1101 13 0.07640258907388642 1101 14 0.10179952437059825 1101 15 0.04188623674340164 1101 16 0.08606134262723941 1101 17 0.04521295869108144 1101 18 0.0864302141230553 1101 19 0.05431336073392856 1101 20 0.11284894681286062 1101 21 -0.021929860526377354 1101 22 -0.18596040224138477 1101 23 -0.3989387546842025 1101 24 -0.06918499439514392 1101 25 -0.03110757959471755 1102 1 -0.6311518590118972 1102 2 -0.5874431994204521 1102 3 -0.2523690802314178 1102 4 -0.2889855615199508 1102 5 -0.5765784271167149 1102 6 -0.36750643486224277 1102 7 -0.38932048762612936 1102 8 -0.3862651162451698 1102 9 -0.47012925968886066 1102 10 -0.42957007851278206 1102 11 -0.30393615084252446 1102 12 -0.2575226909652031 1102 13 -0.37906751253907345 1102 14 -0.25704382545752935 1102 15 -0.3587778360855225 1102 16 -0.3823910428220856 1102 17 -0.4702352338069389 1102 18 -0.34118886947155147 1102 19 -0.29152655162960883 1102 20 -0.3886446504371648 1102 21 -0.2774815547053535 1102 22 -0.3678226770119011 1102 23 -0.25235166595228264 1102 24 -0.33800605736495726 1102 25 -0.28085940220643835 1103 1 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0.6203027768376508 2207 3 0.7566746893410823 2207 4 0.701224911133167 2207 5 0.6790285934935767 2207 6 0.6131723212370943 2207 7 0.46076035135136806 2207 8 0.5135906844976788 2207 9 0.745307343617533 2207 10 0.7186915193315073 2207 11 0.4686980345773293 2207 12 0.5943380620753587 2207 13 0.5621618225421301 2207 14 0.5739968853428508 2207 15 0.5317006432677687 2207 16 0.6228875239246889 2207 17 0.7118068607236907 2207 18 0.6456962809180808 2207 19 0.54324440460082 2207 20 0.44271359004058297 2207 21 0.6899891320618181 2207 22 0.5703959523668729 2207 23 0.672475367767035 2207 24 0.5030106202258935 2207 25 0.4565202496761671 2208 1 0.8552620887563158 2208 2 0.7326016533611437 2208 3 0.7344239458844761 2208 4 0.6912025307899654 2208 5 0.7435344282692355 2208 6 0.5060448926354084 2208 7 0.7200392750881903 2208 8 0.7074091946858871 2208 9 0.7699257211401971 2208 10 0.7891045941342512 2208 11 0.736816218397145 2208 12 0.8225395241834619 2208 13 0.6640244356631939 2208 14 0.6127499964335215 2208 15 0.7556531846769801 2208 16 0.8836756576047406 2208 17 0.7357591234237688 2208 18 0.8067790101493699 2208 19 0.8667184024324612 2208 20 0.7589383633803806 2208 21 0.77932376975388 2208 22 0.8849401096480136 2208 23 0.8437128641786558 2208 24 0.8429242761371754 2208 25 0.8182637982565595 2209 1 0.03323145953291018 2209 2 0.21841305612524664 2209 3 0.2791127298839342 2209 4 0.1831173666078172 2209 5 0.263989452208925 2209 6 0.08605168403749275 2209 7 -0.06431605106889293 2209 8 -0.24793326176424557 2209 9 -0.06088347441822606 2209 10 -0.07521337931136429 2209 11 -0.15429994853551676 2209 12 -0.25308613373361766 2209 13 -0.0056336172418269825 2209 14 -0.07977674471218421 2209 15 -0.0507565406108057 2209 16 -0.14145049268898902 2209 17 -0.10773301552831782 2209 18 -0.16223589697820273 2209 19 -0.09101191099074413 2209 20 -0.1172693176953743 2209 21 -0.1358489016441311 2209 22 -0.23373217124437673 2209 23 -0.34307038822380065 2209 24 -0.23516666073674486 2209 25 -0.055695249588872286 2210 1 0.23560531293622944 2210 2 0.35382935247123015 2210 3 0.4721488005159671 2210 4 0.4361182218389814 2210 5 0.3175175322988082 2210 6 0.35191052058772243 2210 7 0.48715980961227245 2210 8 0.4159434507942379 2210 9 0.47482661898643386 2210 10 0.21896266415450327 2210 11 0.3598643564633903 2210 12 0.1749520330532306 2210 13 0.3493530512135621 2210 14 0.38134780042221905 2210 15 0.5253727837565734 2210 16 0.29932022151287896 2210 17 0.31186687044085915 2210 18 0.25347084257874997 2210 19 0.255011264096872 2210 20 0.37691142115727416 2210 21 0.5075472045386888 2210 22 0.3854008591247151 2210 23 0.44990263007143483 2210 24 0.5483324085705163 2210 25 0.4998401083505257 2211 1 -0.10986310100046381 2211 2 0.0012767035762593082 2211 3 0.04268375191824347 2211 4 -0.050852874875225496 2211 5 -0.051977699592036686 2211 6 0.16608095470990492 2211 7 -0.09323872770927011 2211 8 0.018387577328361715 2211 9 0.08625043890805983 2211 10 0.11220023155130576 2211 11 0.010028858684584918 2211 12 0.1063089390365306 2211 13 -0.009474579921722182 2211 14 0.1318111892661875 2211 15 -0.0016013247395960661 2211 16 -0.025567900608329808 2211 17 -0.1455409673943251 2211 18 -0.03927305447068363 2211 19 -0.05369180366494675 2211 20 -0.1232990762123265 2211 21 -0.16297956267347757 2211 22 0.04569542449511488 2211 23 0.18049087691970556 2211 24 -0.2102215432475134 2211 25 -0.06432034105971951 2212 1 0.02313424714638046 2212 2 0.11505440036010128 2212 3 0.2498569518629452 2212 4 0.26634018254807756 2212 5 0.2720555051285132 2212 6 0.14503642619933999 2212 7 0.14415365858152285 2212 8 0.04531079926710646 2212 9 0.1800229828018871 2212 10 0.1436818759516214 2212 11 0.19036367085545436 2212 12 0.046434242240117385 2212 13 0.12096266164827796 2212 14 0.23143100652711013 2212 15 0.05793592060080499 2212 16 0.23453804783763132 2212 17 0.2633157194230762 2212 18 0.24664133214688633 2212 19 0.2560169952089595 2212 20 0.2035141755126304 2212 21 0.18349636327572227 2212 22 0.25928334411003107 2212 23 0.16076724777697393 2212 24 0.06320776789906546 2212 25 0.07071239864392709 2213 1 0.570317733294754 2213 2 0.6626014978570787 2213 3 0.7020518868551342 2213 4 0.5816550994579686 2213 5 0.7693947545167674 2213 6 0.6161858311542829 2213 7 0.5681195773880958 2213 8 0.5565508122391876 2213 9 0.46758305237941045 2213 10 0.7616516470730232 2213 11 0.6763523497669794 2213 12 0.8169659992691152 2213 13 0.6987951449172414 2213 14 0.7895645993020537 2213 15 0.6747288685061079 2213 16 0.7883918367661593 2213 17 0.7117246740895808 2213 18 0.71987167502256 2213 19 0.7468480928238606 2213 20 0.5921680273360934 2213 21 0.6576897848585119 2213 22 0.6184281695274478 2213 23 0.6801148110895667 2213 24 0.4706760501793994 2213 25 0.42336381328861705 2214 1 0.5939386515006078 2214 2 0.5206153916048919 2214 3 0.5784164513725294 2214 4 0.5429558474546994 2214 5 0.6287651806821302 2214 6 0.46360925251942575 2214 7 0.3924950370026963 2214 8 0.359898234470613 2214 9 0.5078167329374423 2214 10 0.17335150118628917 2214 11 0.34169044762294476 2214 12 0.3476123274690508 2214 13 0.22760003985737853 2214 14 0.32016213993258447 2214 15 0.2456005476790537 2214 16 0.3166178255473966 2214 17 0.25524854073054826 2214 18 0.37244051904274755 2214 19 0.25149600126506755 2214 20 0.2384307805257322 2214 21 0.25060095102421087 2214 22 0.4216601675093088 2214 23 0.34294510888413043 2214 24 0.15066767299675377 2214 25 0.2743975147896123 2215 1 0.3074143540647718 2215 2 0.11248378365289824 2215 3 0.1518592636119287 2215 4 0.29459890578837644 2215 5 0.1964119479247728 2215 6 0.1771998379555484 2215 7 0.10615910082392332 2215 8 -0.09284991544861754 2215 9 -0.03326234963243735 2215 10 0.08985906187885462 2215 11 -0.19158301443363257 2215 12 -0.23900294320549875 2215 13 -0.19557597586466358 2215 14 -0.3428519900775058 2215 15 -0.23717810160161434 2215 16 -0.004382413548938757 2215 17 -0.17333513423839975 2215 18 -0.16841754521001917 2215 19 -0.3175736807368139 2215 20 -0.1847813912270766 2215 21 -0.2122907150106954 2215 22 -0.14510777492096594 2215 23 -0.05882362081455028 2215 24 -0.30206181574531143 2215 25 -0.29895432421108126 2216 1 -0.09871456477845086 2216 2 0.014188677849343634 2216 3 -2.2107188748891247 2216 4 -1.7843410921855352 2216 5 -0.4745005667494766 2216 6 -0.24170118760297044 2216 7 -0.04013405039977869 2216 8 0.039681325054090166 2216 9 -0.052073393008612356 2216 10 -0.14319598218857976 2216 11 -0.07792657951577553 2216 12 0.10955466028891436 2216 13 -0.05491654352709219 2216 14 -0.06987822792842024 2216 15 -0.22799753536936862 2216 16 0.03971600669765252 2216 17 -0.023089321985331424 2216 18 -0.11024972546042022 2216 19 -0.23290998169444066 2216 20 -0.09066956303941254 2216 21 0.028794791898860694 2216 22 -0.08103450201274853 2216 23 -0.1731540282371853 2216 24 -0.05767140033750451 2216 25 -0.020258648719014616 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18533.0 3 14.0 4 307.0 5 2041.0 6 2664.0 7 1968.0 8 1474.0 9 1279.0 10 1276.0 11 1079.0 12 962.0 13 976.0 14 974.0 15 953.0 16 1011.0 17 1034.0 18 1116.0 19 1403.0 20 1788.0 21 2380.0 22 2990.0 23 3669.0 24 4334.0 25 5124.0 26 6316.0 27 6810.0 28 7839.0 29 9812.0 30 12388.0 31 15923.0 32 20823.0 33 27315.0 34 37845.0 35 51908.0 36 76545.0 37 132903.0 38 312041.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.265890232711385 19.67254891109192 10.675945434256876 28.385615421939814 2 13.759838339114596 20.698127900017376 41.145784396378076 24.39624936448995 3 18.534011081778587 24.64249112416144 29.119565678633563 27.703932115426404 4 11.813704251771304 17.43970625481315 38.30001144228012 32.44657805113542 5 12.641861912286364 38.77351079545527 35.718537737486514 12.866089554771845 6 31.542970513644114 38.32284579840714 17.755796523762715 12.378387164186035 7 28.415776269242293 31.584172293669766 21.603705527815247 18.396345909272693 8 24.52547799364818 39.090301261363194 19.738598228176873 16.64562251681175 9 26.017317019230273 15.876162997022112 20.01306362603056 38.09345635771705 10 14.296018298162855 30.133983386111552 34.467325719384455 21.102672596341137 11 34.065203611822895 23.004606707046605 24.61946131227508 18.31072836885542 12 23.37139727492691 25.09481701280769 32.00340955549784 19.530376156767556 13 29.13697321578948 22.602464101083942 25.55987395363129 22.70068872949529 14 19.954076542524195 23.31515422670657 27.631213551791166 29.099555678978064 15 22.248492866427274 32.71775171284171 22.932986715110935 22.100768705620073 16 21.208338261687913 29.008552195131987 28.824060312774808 20.95904923040529 17 20.763908173655466 29.915429870303516 28.29139539428348 21.029266561757538 18 20.649950566524346 28.347944814499115 30.767806657898493 20.23429796107805 19 23.07055414276421 27.443064794411004 27.615342485478532 21.871038577346255 20 22.972893053298503 28.133163968485796 28.561290189866906 20.332652788348796 21 21.886431461697008 27.436150966977095 27.42727902763545 23.250138543690444 22 22.383156963655974 29.33119229844552 27.460058085642252 20.82559265225625 23 21.283595713633705 28.884246253929273 28.258390491312202 21.573767541124816 24 22.454767638146247 27.473814534781315 29.21201259422204 20.8594052328504 25 21.756392586040196 28.31252185719282 28.553079549896115 21.378006006870873 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 230.0 1 230.0 2 244.0 3 258.0 4 258.0 5 258.0 6 428.0 7 598.0 8 598.0 9 598.0 10 950.0 11 1302.0 12 1302.0 13 1302.0 14 2275.5 15 3249.0 16 3249.0 17 3249.0 18 5916.5 19 8584.0 20 8584.0 21 8584.0 22 14014.0 23 19444.0 24 19444.0 25 19444.0 26 28579.0 27 37714.0 28 37714.0 29 37714.0 30 49917.0 31 62120.0 32 62120.0 33 62120.0 34 71553.0 35 80986.0 36 80986.0 37 80986.0 38 87334.5 39 93683.0 40 93683.0 41 93683.0 42 98030.0 43 102377.0 44 102377.0 45 102377.0 46 103912.0 47 105447.0 48 105447.0 49 105447.0 50 99885.5 51 94324.0 52 94324.0 53 94324.0 54 81441.5 55 68559.0 56 68559.0 57 68559.0 58 57519.5 59 46480.0 60 46480.0 61 46480.0 62 37017.0 63 27554.0 64 27554.0 65 27554.0 66 21111.0 67 14668.0 68 14668.0 69 14668.0 70 10540.5 71 6413.0 72 6413.0 73 6413.0 74 4452.5 75 2492.0 76 2492.0 77 2492.0 78 1715.5 79 939.0 80 939.0 81 939.0 82 607.5 83 276.0 84 276.0 85 276.0 86 186.5 87 97.0 88 97.0 89 97.0 90 58.0 91 19.0 92 19.0 93 19.0 94 11.0 95 3.0 96 3.0 97 3.0 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1087659436602697 2 0.1133942816883663 3 0.018513352112386333 4 0.0 5 0.004628338028096583 6 0.3356830719822272 7 0.021598910797784053 8 0.011185150234566742 9 0.010928020344116931 10 0.004371208137646773 11 0.004885467918546394 12 3.8569483567471526E-4 13 0.0010285195617992407 14 1.2856494522490509E-4 15 0.0020570391235984814 16 6.428247261245254E-4 17 1.2856494522490509E-4 18 5.142597808996203E-4 19 3.8569483567471526E-4 20 0.0011570845070241458 21 0.010799455398892027 22 0.0 23 2.5712989044981017E-4 24 0.0 25 0.0052711627542211086 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 777817.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.18666219161656 #Duplication Level Percentage of deduplicated Percentage of total 1 92.436768721652 76.89506253732652 2 5.768109491958754 9.596595515836597 3 0.8651633629267544 2.1591015723705267 4 0.3055056775611501 1.016559903876013 5 0.15785668539732325 0.6565785381417709 6 0.10276203668977202 0.5129058499340743 7 0.06557098161207649 0.3818241767855555 8 0.04852216167752998 0.32291173378205507 9 0.037304629954888126 0.2792922885216485 >10 0.17844473268728747 2.710587522945344 >50 0.017923649198759113 1.0445843210711645 >100 0.013286891450649176 2.1050674421542097 >500 0.001699486086946025 0.9027409226356892 >1k 0.0010814911462383797 1.4161876746188296 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1887 0.24260205163939588 No Hit CTGTAGGACGTGGAATATGGCAAGA 1842 0.23681662910427517 No Hit GTCCTACAGTGTGCATTTCTCATTT 1648 0.2118750297306436 No Hit GTATCAACGCAGAGTACTTTTTTTT 1553 0.19966135993427758 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1440 0.18513352112386333 No Hit CTTTAGGACGTGAAATATGGCGAGG 1419 0.18243365727414032 No Hit CTGTAGGACCTGGAATATGGCGAGA 1230 0.15813488262663325 No Hit GGTATCAACGCAGAGTACTTTTTTT 963 0.12380804225158361 No Hit TATCAACGCAGAGTACTTTTTTTTT 833 0.10709459937234594 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.2856494522490509E-4 0.0 10 0.0 0.0 0.0 1.2856494522490509E-4 0.0 11 0.0 0.0 0.0 1.2856494522490509E-4 0.0 12 0.0 0.0 0.0 1.2856494522490509E-4 0.0 13 0.0 0.0 0.0 1.2856494522490509E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 420 0.0 14.742346 4 AGGACCT 660 0.0 12.845428 5 GGACCTG 620 0.0 12.752312 6 ACTGGAC 60 3.9931823E-4 12.701919 3 GGCGAGG 330 0.0 12.362953 19 GTCCTAC 995 0.0 12.264618 1 TGTAGGA 1065 0.0 12.264168 2 GTAGGAC 1000 0.0 12.193841 3 CTAGGAC 125 1.3442332E-9 12.193841 3 TAGACAA 95 1.0040621E-6 12.0326185 5 GATATAC 185 0.0 11.852863 1 ACTGTTC 185 0.0 11.793427 8 CTGTAGG 1095 0.0 11.75403 1 TCCTACA 1100 0.0 11.7006035 2 TACACTG 375 0.0 11.685009 5 TCAGTAC 75 2.0184743E-4 11.4317255 3 TGGCGAG 955 0.0 11.425157 18 TAGGACA 315 0.0 11.189062 4 GGCGAGA 620 0.0 11.171175 19 CTACTAA 60 0.0057178456 11.122795 1 >>END_MODULE