FastQCFastQC Report
Thu 26 May 2016
SRR1926929_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926929_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644518
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT13440.20852792319221497No Hit
GTATCAACGCAGAGTACTTTTTTTT10890.16896347347940632No Hit
GTCCTAAAGTGTGTATTTCTCATTT10020.15546501416562455No Hit
CTGTAGGACGTGGAATATGGCAAGA9840.15267222948001452No Hit
GTCCTACAGTGTGCATTTCTCATTT8410.13048510669989047No Hit
CTTTAGGACGTGAAATATGGCGAGG7250.11248716094818144No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCTG250.00596561519.034425
CGGTTTC250.00609558818.96353513
GCCGGTT307.8163575E-418.96353511
CCGGTTT250.00609558818.96353512
TAGGACC2450.014.76713854
GGACCTG3600.014.011456
TAAGCCA551.9211517E-413.8486084
TGTAGGA6950.013.0334972
AGGACCT4100.012.5348635
TCTGGAC701.052188E-412.2593273
TCCAACG701.1078907E-412.19179118
GTAGGAC6600.012.1354953
CTGTAGG6850.012.1196421
GGTATCA3050.011.8889881
GTGTAAT1052.5576082E-711.8144951
GTCTTAT907.080738E-611.6630281
GTATAAA1406.2755134E-1011.5872941
GTTATGG855.0753173E-511.2264431
GTGCTAC600.00567754611.1328891
GTATAAC600.00567754611.1328891
Produced by FastQC (version 0.11.5)