##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926929_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 644518 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.715055281621304 31.0 30.0 33.0 23.0 34.0 2 28.757275979879537 31.0 30.0 31.0 23.0 34.0 3 28.46493503672512 31.0 28.0 33.0 16.0 34.0 4 32.33086275325127 35.0 33.0 37.0 24.0 37.0 5 32.254230293025174 35.0 33.0 37.0 22.0 37.0 6 31.816385267750473 35.0 32.0 37.0 18.0 37.0 7 31.814458246317404 35.0 32.0 37.0 17.0 37.0 8 31.64533341194505 35.0 32.0 37.0 17.0 37.0 9 33.818711036774765 38.0 34.0 39.0 23.0 39.0 10 33.97459652019028 38.0 34.0 39.0 25.0 39.0 11 34.09886768096469 38.0 35.0 39.0 25.0 39.0 12 33.996387998473274 38.0 35.0 39.0 25.0 39.0 13 34.096579149069534 38.0 35.0 39.0 25.0 39.0 14 35.16856938052933 39.0 36.0 41.0 25.0 41.0 15 34.75927592402385 39.0 34.0 41.0 19.0 41.0 16 34.846837481652955 39.0 35.0 41.0 22.0 41.0 17 35.08111953428764 39.0 36.0 41.0 25.0 41.0 18 35.13448654653555 39.0 36.0 41.0 25.0 41.0 19 34.532058065096706 39.0 34.0 41.0 18.0 41.0 20 34.812906699269845 39.0 35.0 41.0 19.0 41.0 21 34.802399622663756 39.0 35.0 41.0 19.0 41.0 22 34.82200497115674 39.0 35.0 41.0 19.0 41.0 23 34.83165869688666 39.0 36.0 41.0 21.0 41.0 24 33.56747367800434 39.0 33.0 41.0 2.0 41.0 25 34.14617435044483 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.2281560123073838 1101 2 -0.2813725292865854 1101 3 -1.2064663490715013 1101 4 -0.48944664522943526 1101 5 -0.15892966257264618 1101 6 -2.1940542973183064 1101 7 -0.4495934540262674 1101 8 -0.16058649979513717 1101 9 -0.06781432469041704 1101 10 -9.190366950290922 1101 11 -3.8579736356169896 1101 12 -1.4567334575370268 1101 13 -0.9265082429863725 1101 14 -0.5970849798264695 1101 15 -2.082012452618315 1101 16 -0.5472501595017079 1101 17 -0.33747984520292107 1101 18 -0.21198625639011937 1101 19 -0.974095695441143 1101 20 -0.15429454193220948 1101 21 -0.11124967970727795 1101 22 0.032821082994537676 1101 23 0.2150193129663407 1101 24 -0.013114644234882178 1101 25 0.2570136546579107 1102 1 0.23366770732118525 1102 2 0.09799385290803642 1102 3 -0.13396388507550938 1102 4 0.26468116611582104 1102 5 0.16961082772135683 1102 6 -0.4708953720490783 1102 7 0.38892663207350253 1102 8 0.7680845246008943 1102 9 0.721336841051631 1102 10 0.6832654907297453 1102 11 0.4329460035023658 1102 12 0.6137780317713748 1102 13 0.39615491640038414 1102 14 0.24867381556791912 1102 15 0.8058040348935975 1102 16 0.7484194705527756 1102 17 0.5134937216414173 1102 18 0.3535922964613576 1102 19 0.744339609418617 1102 20 0.5122336794747255 1102 21 0.501729893024276 1102 22 0.5288111149358343 1102 23 0.045456832950250714 1102 24 -3.0470699923423012 1102 25 -1.2868850297824963 1103 1 -0.059253205748333926 1103 2 -0.2692964939307174 1103 3 -0.02272780475127334 1103 4 -0.08193242324772854 1103 5 -0.4188553518932885 1103 6 0.25858089400199447 1103 7 -0.13799676434924635 1103 8 0.17548233767609744 1103 9 0.060862162073128445 1103 10 0.20491394759678627 1103 11 -0.11145158659569887 1103 12 -0.21398633823076807 1103 13 -0.2173131213570798 1103 14 -0.22470774203669208 1103 15 0.3085387839131499 1103 16 0.17167926515148224 1103 17 -0.09882773269417555 1103 18 -0.12389466374815328 1103 19 -1.7030509022483358 1103 20 -0.5250515957271631 1103 21 -0.5951919294422012 1103 22 -0.4488190315732581 1103 23 -0.5414933207290886 1103 24 1.073796732910516 1103 25 0.4075444766510543 1104 1 -0.266115087696015 1104 2 -0.18714345586352366 1104 3 -1.2213155105906353 1104 4 -0.3461696903762572 1104 5 -0.3823598672171826 1104 6 -1.5090412633526604 1104 7 0.029634368093130803 1104 8 0.47146470376565475 1104 9 0.290390414367117 1104 10 0.21716707926132273 1104 11 0.21207788801976335 1104 12 0.23435027884497117 1104 13 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8 1.0355413065767323 1110 9 0.7004320887494231 1110 10 0.6686272755470668 1110 11 0.5300635797487985 1110 12 0.6105118551839581 1110 13 0.46711558173491596 1110 14 0.3053415210559507 1110 15 0.6104872860378237 1110 16 0.7377868283411857 1110 17 -0.008755387722025887 1110 18 0.22781241659799178 1110 19 -1.401208429958828 1110 20 0.23980277637231495 1110 21 0.15704285323742084 1110 22 0.17247094371363403 1110 23 -0.006609504960003676 1110 24 -0.7652499773099422 1110 25 -0.28430019779657556 1111 1 0.4221581627586559 1111 2 0.052931284414011515 1111 3 0.6365384469070605 1111 4 0.7101994269410667 1111 5 0.4457175637103461 1111 6 0.7107698928794335 1111 7 0.5267180024636922 1111 8 0.8343118100712417 1111 9 0.37805677376820057 1111 10 0.39612121364434216 1111 11 0.12289579579025656 1111 12 0.0374913635755334 1111 13 0.11647706131461888 1111 14 0.09705264167592986 1111 15 -0.6710550521925711 1111 16 -0.36013928776207393 1111 17 0.006950407932421854 1111 18 0.1521018665100371 1111 19 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2216 12 -2.4190624145431876 2216 13 -1.9877904416749317 2216 14 -2.333617243931343 2216 15 -3.5394208381631067 2216 16 -2.4106083656258406 2216 17 -2.605808329460693 2216 18 -2.214202142876033 2216 19 -1.4892766578829395 2216 20 -2.456364226601252 2216 21 -5.230462038089467 2216 22 -3.5487207187604675 2216 23 -3.5832027250850444 2216 24 -3.9740127105383287 2216 25 -3.422362745470103 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30215.0 3 4.0 4 252.0 5 1445.0 6 1886.0 7 1503.0 8 1281.0 9 1170.0 10 1064.0 11 1071.0 12 1042.0 13 983.0 14 1030.0 15 999.0 16 1078.0 17 1238.0 18 1423.0 19 1830.0 20 2331.0 21 3036.0 22 3756.0 23 4524.0 24 5245.0 25 5929.0 26 7071.0 27 7654.0 28 8606.0 29 10414.0 30 12296.0 31 15084.0 32 19805.0 33 26325.0 34 35770.0 35 50343.0 36 75289.0 37 122634.0 38 178892.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.662358562590086 19.24388419741607 12.734303693349236 25.359453546644605 2 13.982211519788713 20.51563288926865 43.158270866508715 22.343884724433916 3 18.663957106923515 25.108328012093583 29.731358959181044 26.496355921801857 4 11.066171904111291 17.53415724754689 40.12389765246554 31.27577319587629 5 11.921559620213483 39.30057948792759 36.62382325463016 12.154037637228763 6 29.872076800476744 38.6699587659904 19.595077990784812 11.862886442748044 7 26.68795577840649 31.775711115468532 23.904653413883363 17.63167969224161 8 23.399931218322692 38.427794022218656 21.654401638311082 16.517873121147563 9 25.780145530952097 15.80402824957829 21.01430945733932 37.40151676213029 10 13.411518558643575 30.28503046802487 36.08807944347351 20.215371529858043 11 33.499736196890225 23.35805840911207 25.254337233481273 17.887868160516433 12 22.162684742059426 25.505577177333755 33.299659224223014 19.032078856383805 13 28.25220662172731 23.10792273410053 26.225590604943328 22.41428003922883 14 19.482502572124226 23.257434331302015 28.950247436022774 28.309815660550985 15 21.978163512955714 32.52817235803646 23.851458507260727 21.642205621747106 16 20.61682567861998 29.44354092824909 29.432057306376823 20.50757608675411 17 20.48115927635234 29.602484163612154 28.998047802458714 20.918308757576792 18 20.062598634747676 28.98158507688273 31.05242984809667 19.90338644027293 19 22.494929100522533 27.87900608818321 28.413953004750425 21.212111806543827 20 22.13590726400894 28.697568318306665 29.519250166819262 19.64727425086513 21 21.90067703977046 27.88385889392526 28.11445277403196 22.101011292272315 22 21.406779555809365 29.559844073940734 28.62487740686025 20.408498963389654 23 21.000670382738885 29.005071321274723 28.806439398641853 21.187818897344542 24 21.94891832798226 27.9957605222689 29.6409634104519 20.41435773929695 25 21.48071133732659 28.42556096955402 29.32900903137705 20.764718661742343 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 160.0 1 160.0 2 229.5 3 299.0 4 299.0 5 299.0 6 505.5 7 712.0 8 712.0 9 712.0 10 1159.5 11 1607.0 12 1607.0 13 1607.0 14 2758.5 15 3910.0 16 3910.0 17 3910.0 18 6765.0 19 9620.0 20 9620.0 21 9620.0 22 14794.0 23 19968.0 24 19968.0 25 19968.0 26 28443.5 27 36919.0 28 36919.0 29 36919.0 30 47417.5 31 57916.0 32 57916.0 33 57916.0 34 64932.5 35 71949.0 36 71949.0 37 71949.0 38 76486.0 39 81023.0 40 81023.0 41 81023.0 42 83225.0 43 85427.0 44 85427.0 45 85427.0 46 84722.0 47 84017.0 48 84017.0 49 84017.0 50 78171.5 51 72326.0 52 72326.0 53 72326.0 54 61678.0 55 51030.0 56 51030.0 57 51030.0 58 42105.5 59 33181.0 60 33181.0 61 33181.0 62 26042.5 63 18904.0 64 18904.0 65 18904.0 66 14073.0 67 9242.0 68 9242.0 69 9242.0 70 6624.0 71 4006.0 72 4006.0 73 4006.0 74 2786.0 75 1566.0 76 1566.0 77 1566.0 78 1032.5 79 499.0 80 499.0 81 499.0 82 335.0 83 171.0 84 171.0 85 171.0 86 110.5 87 50.0 88 50.0 89 50.0 90 31.0 91 12.0 92 12.0 93 12.0 94 7.0 95 2.0 96 2.0 97 2.0 98 2.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21550988490624 2 0.13079541610940268 3 0.13513974784257382 4 0.0679577606831772 5 0.02420413394195352 6 0.02280774159914851 7 0.30037950840783345 8 0.295569712560394 9 0.018463409865977366 10 0.03661651032244251 11 0.015205161066099007 12 0.016446398704147908 13 0.014584542247074558 14 0.016911862818416244 15 0.014274232837562333 16 0.018928873980245703 17 0.01799794575170903 18 0.01473969695183067 19 0.02404897923719741 20 0.01675670811366013 21 0.01473969695183067 22 0.017067017523172353 23 0.017067017523172353 24 0.01536031577085512 25 0.015205161066099007 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 644518.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.78745687055705 #Duplication Level Percentage of deduplicated Percentage of total 1 93.70563949247584 81.32474145981216 2 4.954412147576979 8.599616611536021 3 0.690555674360532 1.7979471241584932 4 0.22091618096699112 0.7669101411072368 5 0.10936713006745667 0.4745847541893 6 0.06422940154982747 0.3344583850096406 7 0.039424071045254874 0.23950604058512845 8 0.02888392827170555 0.20054101433063273 9 0.024835634789485042 0.19398794248308057 >10 0.13616109646294944 2.2298212175702035 >50 0.013950233496591764 0.8533892174030534 >100 0.010015392315264491 1.7750518676042653 >500 0.0010730777474157614 0.6762767562739465 >1k 5.365388737078807E-4 0.5331674679368845 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1344 0.20852792319221497 No Hit GTATCAACGCAGAGTACTTTTTTTT 1089 0.16896347347940632 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1002 0.15546501416562455 No Hit CTGTAGGACGTGGAATATGGCAAGA 984 0.15267222948001452 No Hit GTCCTACAGTGTGCATTTCTCATTT 841 0.13048510669989047 No Hit CTTTAGGACGTGAAATATGGCGAGG 725 0.11248716094818144 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.551547047561123E-4 0.0 0.0 0.0 0.0 7 1.551547047561123E-4 0.0 0.0 0.0 0.0 8 1.551547047561123E-4 0.0 0.0 0.0 0.0 9 1.551547047561123E-4 0.0 0.0 0.0 0.0 10 1.551547047561123E-4 0.0 0.0 0.0 0.0 11 1.551547047561123E-4 0.0 0.0 0.0 0.0 12 1.551547047561123E-4 0.0 0.0 0.0 0.0 13 1.551547047561123E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCGCTG 25 0.005965615 19.03442 5 CGGTTTC 25 0.006095588 18.963535 13 GCCGGTT 30 7.8163575E-4 18.963535 11 CCGGTTT 25 0.006095588 18.963535 12 TAGGACC 245 0.0 14.7671385 4 GGACCTG 360 0.0 14.01145 6 TAAGCCA 55 1.9211517E-4 13.848608 4 TGTAGGA 695 0.0 13.033497 2 AGGACCT 410 0.0 12.534863 5 TCTGGAC 70 1.052188E-4 12.259327 3 TCCAACG 70 1.1078907E-4 12.191791 18 GTAGGAC 660 0.0 12.135495 3 CTGTAGG 685 0.0 12.119642 1 GGTATCA 305 0.0 11.888988 1 GTGTAAT 105 2.5576082E-7 11.814495 1 GTCTTAT 90 7.080738E-6 11.663028 1 GTATAAA 140 6.2755134E-10 11.587294 1 GTTATGG 85 5.0753173E-5 11.226443 1 GTGCTAC 60 0.005677546 11.132889 1 GTATAAC 60 0.005677546 11.132889 1 >>END_MODULE