FastQCFastQC Report
Thu 26 May 2016
SRR1926929_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926929_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644518
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16460.2553846440285609No Hit
GTCCTACAGTGGACATTTCTAAATT13330.2068212214398977No Hit
CTGTAGGACGTGGAATATGGCAAGA12380.19208152448806706No Hit
GGTATCAACGCAGAGTACTTTTTTT10400.1613608929463568No Hit
GTCCTAAAGTGTGTATTTCTCATTT10030.15562016887038066No Hit
CTTTAGGACGTGAAATATGGCGAGG9750.15127583713720952No Hit
TATCAACGCAGAGTACTTTTTTTTT9010.1397943889852572No Hit
GTCCTACAGTGTGCATTTCTCATTT8490.13172634433793937No Hit
CTGTAGGACCTGGAATATGGCGAGA6870.10659128216744918No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAT402.685667E-416.6829781
GCCTAAG400.005154197614.2996961
TCCTATA759.284013E-713.9808342
CCTAGAC707.0927163E-613.5996753
GTCCTAT852.5789996E-713.4585371
TAAGGCT801.948185E-613.0896874
TAGGACC2450.012.8225524
GACGTGG3500.012.7440127
GGTATCA3600.012.7108411
GTCTTAT1051.8908395E-812.710841
TCCAACG751.4922985E-512.65264718
TGTAGGA7550.012.6256792
CTTAGGG701.068047E-412.2397083
GTAGGAC7200.012.1641543
ACGTGGA3850.012.0765918
GTCCTAA3500.011.9845071
GCTATAT802.7601023E-511.9164131
GATATAC1950.011.7330841
CTAAGGC657.8736385E-411.7166433
CTGTAGG7650.011.7139111
Produced by FastQC (version 0.11.5)