##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926929_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 644518 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.873472889818437 31.0 30.0 33.0 26.0 34.0 2 30.159401289025286 31.0 30.0 34.0 26.0 34.0 3 30.203964823325336 31.0 30.0 34.0 26.0 34.0 4 33.771402815747585 35.0 35.0 37.0 28.0 37.0 5 33.81229228663901 37.0 35.0 37.0 30.0 37.0 6 33.75244911701458 37.0 35.0 37.0 29.0 37.0 7 33.750666389456924 37.0 35.0 37.0 29.0 37.0 8 33.79993731749928 37.0 35.0 37.0 29.0 37.0 9 35.3167219534598 39.0 35.0 39.0 30.0 39.0 10 35.19943275439941 39.0 35.0 39.0 29.0 39.0 11 35.2373882498239 39.0 35.0 39.0 29.0 39.0 12 35.13983007456735 39.0 35.0 39.0 29.0 39.0 13 35.153798652636546 39.0 35.0 39.0 29.0 39.0 14 36.24876884741776 40.0 36.0 41.0 29.0 41.0 15 36.243326020374916 40.0 36.0 41.0 29.0 41.0 16 36.222557011596265 40.0 36.0 41.0 29.0 41.0 17 36.20975830000093 40.0 36.0 41.0 29.0 41.0 18 36.18211438625453 40.0 36.0 41.0 29.0 41.0 19 36.25763283570048 40.0 37.0 41.0 29.0 41.0 20 36.22188208863057 40.0 37.0 41.0 29.0 41.0 21 36.17389118690246 40.0 37.0 41.0 29.0 41.0 22 36.08474394819074 40.0 37.0 41.0 29.0 41.0 23 36.01039846831276 40.0 37.0 41.0 28.0 41.0 24 35.937773654110515 40.0 36.0 41.0 28.0 41.0 25 35.81794612408032 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.044011849654278734 1101 2 0.0798395395081215 1101 3 0.324563184211371 1101 4 0.12508030588264774 1101 5 -0.12396311547929884 1101 6 -0.08346480713713333 1101 7 0.04517412203058058 1101 8 0.06067804489248374 1101 9 -0.017980579135851826 1101 10 -0.011639001388658698 1101 11 -0.050085765067393595 1101 12 0.07029300023793184 1101 13 0.17384717712840825 1101 14 0.15107437058244244 1101 15 0.19191474449014834 1101 16 -0.022293620660335023 1101 17 -0.0582473912596555 1101 18 0.2408107501971557 1101 19 0.04599713050936316 1101 20 -0.17319615586647075 1101 21 -0.04491897203789108 1101 22 -0.23515010956138838 1101 23 -0.12996255979083315 1101 24 -0.1201841334537761 1101 25 -0.10311343720626809 1102 1 -0.4875025766714174 1102 2 -0.6303450871346783 1102 3 -0.45106319818102847 1102 4 -0.7448800394393871 1102 5 -0.8336763689758655 1102 6 -0.769091881755827 1102 7 -0.7602081022573373 1102 8 -0.7191478437118235 1102 9 -0.7106782509480638 1102 10 -0.7308060883355836 1102 11 -0.6575506016195831 1102 12 -0.7137258019468646 1102 13 -0.592101222345498 1102 14 -0.7271300603251305 1102 15 -0.6844701201466492 1102 16 -0.6579056970425654 1102 17 -0.4741336551824702 1102 18 -0.5215446651551048 1102 19 -0.5483450732095392 1102 20 -0.6950721550621637 1102 21 -0.8284894752864105 1102 22 -0.7933581004899324 1102 23 -0.8805435354343558 1102 24 -0.5881851487190133 1102 25 -0.7343381173812418 1103 1 -0.8238920084294818 1103 2 -0.7354033890981881 1103 3 -0.5946091030528606 1103 4 -0.605700770192307 1103 5 -0.5941475502737674 1103 6 -0.5462830693495562 1103 7 -0.5416030647228496 1103 8 -0.5851495138543115 1103 9 -0.5353373363920326 1103 10 -0.5712998296733574 1103 11 -0.5606714071354233 1103 12 -0.821012782268042 1103 13 -0.6793716324048233 1103 14 -0.8740665293291272 1103 15 -0.6964223505738474 1103 16 -0.7211087854945362 1103 17 -0.8708373098214821 1103 18 -0.8451150975121422 1103 19 -0.8248061199779002 1103 20 -0.789684415307093 1103 21 -0.7402440867596809 1103 22 -0.6957542303063491 1103 23 -0.8636672265366983 1103 24 -1.0533056219551966 1103 25 -0.7876340255321495 1104 1 -0.20870530523563247 1104 2 -0.20175856323626107 1104 3 0.046441879719246515 1104 4 -0.11282528030210415 1104 5 -0.07348241079662898 1104 6 -0.15225321222440158 1104 7 -0.08983133966825108 1104 8 -0.174973046534312 1104 9 -0.10268041125708294 1104 10 -0.07987210690954072 1104 11 -0.17293693296871737 1104 12 -0.06157077629319474 1104 13 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2214 12 0.1392633297964423 2214 13 0.45039236119369264 2214 14 0.1754707130209141 2214 15 0.29301424198732207 2214 16 0.02968465105946194 2214 17 0.09253252994867722 2214 18 0.08367806909988929 2214 19 0.16039720416454628 2214 20 0.0897325100539561 2214 21 0.018135683018392967 2214 22 0.20148946944106427 2214 23 0.05390792337750128 2214 24 -0.2565150390733777 2214 25 -0.12999370391558074 2215 1 0.4801045288667254 2215 2 0.4027820839235261 2215 3 0.3762123532324537 2215 4 0.34046549418200556 2215 5 0.7135856265619935 2215 6 0.5872864401397351 2215 7 0.5618569094628469 2215 8 0.5670788218251843 2215 9 0.40711665995029733 2215 10 0.5827017768964211 2215 11 0.3382414286552091 2215 12 0.32793027419197074 2215 13 0.42927752317118006 2215 14 0.2902666467267494 2215 15 0.39144278335245986 2215 16 0.3262226319133532 2215 17 0.3691547743128609 2215 18 0.24794896640749187 2215 19 0.35978562802461056 2215 20 0.4308562812110992 2215 21 0.41562925159093567 2215 22 0.46956940381688383 2215 23 0.5001506383632659 2215 24 0.44816154280009357 2215 25 0.5091875399375212 2216 1 -0.07866927572034399 2216 2 0.02093275556872598 2216 3 -1.7521679649465831 2216 4 -1.1804649549120185 2216 5 -0.2848366689032673 2216 6 0.0018166678378435108 2216 7 0.2034222682440685 2216 8 0.005460838407465474 2216 9 -0.02114390786815079 2216 10 0.1355122031956384 2216 11 0.03861292203445288 2216 12 0.10794653403215193 2216 13 0.10298063711162087 2216 14 0.018879913411403493 2216 15 8.815947115934364E-4 2216 16 0.07322003602900651 2216 17 0.12053612791437729 2216 18 -0.08922890813999373 2216 19 -0.1341005513698832 2216 20 -0.026509917805555006 2216 21 0.008734079280600326 2216 22 0.04072775793482464 2216 23 -0.24186190912952554 2216 24 -0.35854464981952816 2216 25 -0.2755868353820077 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15770.0 3 10.0 4 223.0 5 1572.0 6 2253.0 7 1742.0 8 1277.0 9 1200.0 10 1059.0 11 901.0 12 860.0 13 811.0 14 776.0 15 805.0 16 830.0 17 893.0 18 981.0 19 1166.0 20 1553.0 21 1939.0 22 2495.0 23 2925.0 24 3704.0 25 4265.0 26 5095.0 27 5640.0 28 6451.0 29 7976.0 30 10073.0 31 13177.0 32 16827.0 33 22306.0 34 30512.0 35 42435.0 36 63057.0 37 110038.0 38 260921.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.113461759506926 20.129264951211102 10.596557283851666 28.16071600543031 2 13.635805872896295 21.542081841551404 40.912232741280356 23.909879544271945 3 18.188815214709457 25.377970265149457 29.726315854811002 26.70689866533008 4 11.520081673436582 18.189716966787582 39.156703148709575 31.13349821106625 5 12.168978855920171 39.23718701656987 36.11933906712822 12.474495060381733 6 30.16407327713717 39.34769272927131 18.432475859212737 12.055758134378786 7 27.255219333843385 32.44747300906449 22.331129157659344 17.966178499432793 8 23.94767671908822 39.2624775973497 20.38637298182185 16.403472701740228 9 25.917132952856214 15.967159797285765 20.437145242329166 37.67856200752885 10 13.697813954137878 30.797660811454513 35.14275473741538 20.36177049699223 11 33.55159139608098 23.45953511032635 24.95155136486283 18.037322128729848 12 22.51150089602259 25.867978247209138 32.6239110028471 18.996609853921164 13 28.47552035251313 23.06819856791541 26.074800431332086 22.381480648239375 14 19.687735641207848 23.5728094483009 28.45335584110917 28.286099069382082 15 21.86473524396469 32.565139744204416 23.740455764865423 21.829669246965473 16 20.72749163713546 29.66117210433876 29.015416219302548 20.59592003922323 17 20.382705188536534 29.783233025661076 28.790396529494178 21.043665256308213 18 19.952367283926673 29.10141734482518 30.860414420145382 20.085800951102765 19 22.22132577003519 27.95337896964544 28.482613309832495 21.342681950486877 20 22.18164045832913 28.732923205821432 29.2241452875418 19.861291048307642 21 21.811165111818706 27.824640460806037 28.104109640057352 22.2600847873179 22 21.633841102963768 29.536335680306834 28.44342594000478 20.386397276724622 23 21.057131065385295 29.03875454215398 28.59470177714199 21.309412615318735 24 21.67700774378333 28.027034197701532 29.706741637536325 20.589216420978808 25 21.448454568023834 28.59033639523337 29.04962140019861 20.91158763654419 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 257.0 1 257.0 2 258.0 3 259.0 4 259.0 5 259.0 6 467.0 7 675.0 8 675.0 9 675.0 10 1136.5 11 1598.0 12 1598.0 13 1598.0 14 2592.0 15 3586.0 16 3586.0 17 3586.0 18 6261.5 19 8937.0 20 8937.0 21 8937.0 22 14010.5 23 19084.0 24 19084.0 25 19084.0 26 26996.5 27 34909.0 28 34909.0 29 34909.0 30 45407.0 31 55905.0 32 55905.0 33 55905.0 34 63202.5 35 70500.0 36 70500.0 37 70500.0 38 75369.0 39 80238.0 40 80238.0 41 80238.0 42 83025.5 43 85813.0 44 85813.0 45 85813.0 46 85334.5 47 84856.0 48 84856.0 49 84856.0 50 79905.0 51 74954.0 52 74954.0 53 74954.0 54 63885.0 55 52816.0 56 52816.0 57 52816.0 58 43724.5 59 34633.0 60 34633.0 61 34633.0 62 27027.0 63 19421.0 64 19421.0 65 19421.0 66 14516.0 67 9611.0 68 9611.0 69 9611.0 70 6890.0 71 4169.0 72 4169.0 73 4169.0 74 2875.5 75 1582.0 76 1582.0 77 1582.0 78 1036.0 79 490.0 80 490.0 81 490.0 82 324.0 83 158.0 84 158.0 85 158.0 86 105.5 87 53.0 88 53.0 89 53.0 90 33.0 91 13.0 92 13.0 93 13.0 94 7.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11233200624342532 2 0.11062530449110809 3 0.013808768723293997 4 0.0 5 0.005430414666463931 6 0.34816715747271604 7 0.02094588514207516 8 0.009774746399635076 9 0.009929901104391188 10 0.003258248799878359 11 0.003258248799878359 12 4.65464114268337E-4 13 4.65464114268337E-4 14 0.0 15 0.002947939390366134 16 3.103094095122246E-4 17 1.551547047561123E-4 18 4.65464114268337E-4 19 3.103094095122246E-4 20 4.65464114268337E-4 21 0.01272268579000121 22 0.0 23 0.0 24 1.551547047561123E-4 25 0.005895878780732268 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 644518.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.58258337337907 #Duplication Level Percentage of deduplicated Percentage of total 1 92.34856717225568 78.11080382259415 2 6.0163686304845765 10.177600025858883 3 0.8605014482484982 2.1835030646817617 4 0.28713850999850754 0.9714766784662644 5 0.13049112853783318 0.55186383795188 6 0.07283330092326656 0.3696257248620361 7 0.047533217384107734 0.28143376256773706 8 0.03684456822109961 0.24931270107338485 9 0.024229887824373568 0.18444838563294297 >10 0.14591827775140767 2.326172315069326 >50 0.016164684847875702 0.948838815513688 >100 0.011204925821470736 1.8852288913618855 >500 0.0012858111590795924 0.7870916071109455 >1k 9.184365421997089E-4 0.9726003672551361 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1646 0.2553846440285609 No Hit GTCCTACAGTGGACATTTCTAAATT 1333 0.2068212214398977 No Hit CTGTAGGACGTGGAATATGGCAAGA 1238 0.19208152448806706 No Hit GGTATCAACGCAGAGTACTTTTTTT 1040 0.1613608929463568 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1003 0.15562016887038066 No Hit CTTTAGGACGTGAAATATGGCGAGG 975 0.15127583713720952 No Hit TATCAACGCAGAGTACTTTTTTTTT 901 0.1397943889852572 No Hit GTCCTACAGTGTGCATTTCTCATTT 849 0.13172634433793937 No Hit CTGTAGGACCTGGAATATGGCGAGA 687 0.10659128216744918 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.551547047561123E-4 0.0 10 0.0 0.0 0.0 1.551547047561123E-4 0.0 11 0.0 0.0 0.0 1.551547047561123E-4 0.0 12 0.0 0.0 0.0 1.551547047561123E-4 0.0 13 0.0 0.0 0.0 1.551547047561123E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCTTAT 40 2.685667E-4 16.682978 1 GCCTAAG 40 0.0051541976 14.299696 1 TCCTATA 75 9.284013E-7 13.980834 2 CCTAGAC 70 7.0927163E-6 13.599675 3 GTCCTAT 85 2.5789996E-7 13.458537 1 TAAGGCT 80 1.948185E-6 13.089687 4 TAGGACC 245 0.0 12.822552 4 GACGTGG 350 0.0 12.744012 7 GGTATCA 360 0.0 12.710841 1 GTCTTAT 105 1.8908395E-8 12.71084 1 TCCAACG 75 1.4922985E-5 12.652647 18 TGTAGGA 755 0.0 12.625679 2 CTTAGGG 70 1.068047E-4 12.239708 3 GTAGGAC 720 0.0 12.164154 3 ACGTGGA 385 0.0 12.076591 8 GTCCTAA 350 0.0 11.984507 1 GCTATAT 80 2.7601023E-5 11.916413 1 GATATAC 195 0.0 11.733084 1 CTAAGGC 65 7.8736385E-4 11.716643 3 CTGTAGG 765 0.0 11.713911 1 >>END_MODULE