FastQCFastQC Report
Thu 26 May 2016
SRR1926928_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926928_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315027
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT8420.2672786777006415No Hit
CTGTAGGACGTGGAATATGGCAAGA7540.23934456411672653No Hit
GTCCTAAAGTGTGTATTTCTCATTT7480.23743996546327772No Hit
GTCCTACAGTGTGCATTTCTCATTT6600.20950585187936274No Hit
CTTTAGGACGTGAAATATGGCGAGG5640.17903227342418268No Hit
GTATCAACGCAGAGTACTTTTTTTT4960.15744682201843016No Hit
CTGTAGGACCTGGAATATGGCGAGA4660.1479238287511864No Hit
GTCCTTCAGTGTGCATTTCTCATTT3210.10189602795950822No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA456.5471226E-414.834771
CCCTTAA456.5471226E-414.834771
GTCCAGA456.5471226E-414.834771
TAGGACC1900.014.0315914
AATCCCG707.3482624E-613.54806919
TTTAGAA1404.9112714E-1112.2555222
CCAGGAC550.002982797312.1317293
AAATCCC802.9000732E-511.85456118
GTATAAT657.738099E-411.7374011
TCCTATA657.76927E-411.7317822
CACCTTT907.5511816E-611.591125514
TGTCCAC1406.85759E-1011.51768710
AGGACCT2900.011.4840695
GTATATG1001.8266637E-611.4439661
CACTGTT1001.8764404E-611.4184467
TAAACAG752.0330235E-411.4184455
ACTGTTC1001.8945157E-611.4093588
TGTAGGA5100.011.4011072
CTGTAGG5050.011.3306591
GGACCTG2700.011.2774766
Produced by FastQC (version 0.11.5)